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一步法TUNEL细胞凋亡检测试剂盒(绿色荧光)
细胞凋亡中, 染色体DNA双链断裂或单链断裂而产生大量的粘性3'-OH末端,可在脱氧核糖核苷酸末端转移酶(TdT)的作用下,将脱氧核糖核苷酸和荧光素、过氧化物酶、碱性磷酸酶或生物素形成的衍生物标记到DNA的3'-末端,从而可进行凋亡细胞的检测,这类方法称为脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(terminal -deoxynucleotidyl transferase mediated nick end labeling, TUNEL)。由于正常的或正在增殖的细胞几乎没有DNA的断裂,因而没有3'-OH形成,很少能够被染色。由此,TUNEL成为了检测DNA片段化(细胞凋亡)的最常用方法。
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光)具有如下优势和特点:
- 提供优化的阳性对照品,解决凋亡检测非标准化的问题。
- 独家优化的实验方案,分别适用于荧光酶标仪、荧光显微镜、流式细胞仪。
- 基于最广泛使用的FITC通道(Ex/Em = 490nm/520 nm),检测方便。
产品组分
内容 |
型号 |
规格 |
储存温度 |
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光) | KTA2010-01 | 50T | 2-8°C |
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光) | KTA2010-02 | 100T | 2-8°C |
操作手册 |
1 | 1 |
常温 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
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