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小麦胚芽凝集素,AF594标记
XFD594由AAT生产,它与Alexa Fluor® 594(Alexa Fluor®是ThermoFisher的商标)具有相同的化学结构。小麦胚芽凝集素(WGA)是一种凝集素,与N-乙酰-D-葡糖胺和硅酸结合。由于WGA与糖类结合,其衍生物和共轭物被广泛用于标记细胞膜和纤维化的疤痕组织,用于荧光成像和分析。WGA的XFD594共轭物等同于WGA的Alexa Fluor® 594共轭物。它表现出明亮的红色荧光。XFD594 WGA共轭物能与唾液酸和N-乙酰葡糖胺基残基结合。
产品物理化学光谱特性
Ex(nm):590
Em(nm):618
量子产率(cm -1 M -1):92000
消光系数:0.66
图1. 活的HeLa细胞用小麦胚芽凝集素、XFD594标记的5微克/毫升染色30分钟,然后用Hoechst 33342(Cat No. 17535)。使用Cy3/TRITC和DAPI滤光片组,用荧光显微镜获得图像。
产品组分
内容 |
型号 |
规格 |
储存温度 |
小麦胚芽凝集素,AF594标记 | 25509 | 1mg | -20°C |
操作手册 |
1 | 1 |
常温 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
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Journal: Plant physiology and biochemistry : PPB (2020): 420-427
Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
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Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)
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Journal: Journal of food science (2020): 808-815
Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572
Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)
Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242
Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)‑poly(ε‑caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128
Wheat Germ Agglutinin as a Potential Therapeutic Agent for Leukemia.
Authors: Ryva, Bradley and Zhang, Keman and Asthana, Abhishek and Wong, Derek and Vicioso, Yorleny and Parameswaran, Reshmi
Journal: Frontiers in oncology (2019): 100
Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and
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