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DMEM低糖,无氨基酸,丙酮酸(粉)
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d9800-13-01

DMEM低糖,无氨基酸,丙酮酸(粉)

DMEM低糖,无氨基酸,丙酮酸(粉),Dulbecco’s MEM (DMEM) Low Glucose, w/o Amino Acids, Pyruvic Acid (Powder)。 更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
d9800-13-01
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Dulbecco's MEM是最广泛使用的修饰BME。它含有4倍高浓度的氨基酸和维生素。非必需的氨基酸和某些必需的微量元素被添加。碳酸氢盐的浓度被增加。DMEM的标准配方是含有1000mg/ml葡萄糖。DMEM最初是为培养小鼠胚胎细胞而开发的。今天,它在正常和转化的小鼠和鸡细胞的无血清培养中得到了广泛的应用。

Appearance  Light tan to orange, homogeneous, free-flowing powder
Solubility Yellow to orange, clear, complete
pH As Reported
Endotoxin ≤1EU/ml
Directions per Liter Dissolve 8.32g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Storage and Stability

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/Liter

Inorganic Salts

Calcium Chloride•2H2O 0.265
Ferric Nitrate•9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic 0.109

Amino Acids

L-Arginine•HCl Absent
L-Cystine•2HCl Absent
L-Glutamine Absent
Glycine Absent
L-Histidine•HCl•H2O Absent
L-Isoleucine Absent
L-Leucine Absent
L-Lysine•HCl Absent
L-Methionine Absent
L-Phenylalanine Absent
L-Serine Absent
L-Threonine Absent
L-Tryptophan Absent
L-Tyrosine•2Na•2H2O Absent
L-Valine Absent

Vitamins

Choline Chloride 0.004
Folic Acid 0.004
myo-Inositol 0.0072
Niacinamide 0.004
D-Pantothenic Acid•Ca 0.004
Pyridoxal•HCl 0.004
Riboflavin 0.0004
Thiamine•HCl 0.004

Other

D-Glucose 1
Phenol Red, Sodium 0.0159
Pyruvic Acid, Sodium Absent

产品组分

组分内容

型号 规格

储存温度

DMEM低糖,无氨基酸,丙酮酸(粉)

D9800-13-10L 10L 2-8°C

DMEM低糖,无氨基酸,丙酮酸(粉)

D9800-13-25L 25L 2-8°C

DMEM低糖,无氨基酸,丙酮酸(粉)

D9800-13-50L 50L 2-8°C

DMEM低糖,无氨基酸,丙酮酸(粉)

D9800-13-100L 100L 2-8°C
操作手册  1  1

常温

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D9800-13-DMEM低糖,无氨基酸,丙酮酸(粉) 操作手册
D9800-13-DMEM低糖,无氨基酸,丙酮酸(粉)相关说明 宣传单页

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