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DMEM低糖,无氨基酸,丙酮酸(粉)
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Dulbecco's MEM是最广泛使用的修饰BME。它含有4倍高浓度的氨基酸和维生素。非必需的氨基酸和某些必需的微量元素被添加。碳酸氢盐的浓度被增加。DMEM的标准配方是含有1000mg/ml葡萄糖。DMEM最初是为培养小鼠胚胎细胞而开发的。今天,它在正常和转化的小鼠和鸡细胞的无血清培养中得到了广泛的应用。
Appearance | Light tan to orange, homogeneous, free-flowing powder |
Solubility | Yellow to orange, clear, complete |
pH | As Reported |
Endotoxin | ≤1EU/ml |
Directions per Liter | Dissolve 8.32g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as | g/Liter |
Inorganic Salts
Calcium Chloride•2H2O | 0.265 |
Ferric Nitrate•9H2O | 0.0001 |
Magnesium Sulfate | 0.09767 |
Potassium Chloride | 0.4 |
Sodium Chloride | 6.4 |
Sodium Phosphate Monobasic 0.109 |
Amino Acids
L-Arginine•HCl | Absent |
L-Cystine•2HCl | Absent |
L-Glutamine | Absent |
Glycine | Absent |
L-Histidine•HCl•H2O | Absent |
L-Isoleucine | Absent |
L-Leucine | Absent |
L-Lysine•HCl | Absent |
L-Methionine | Absent |
L-Phenylalanine | Absent |
L-Serine | Absent |
L-Threonine | Absent |
L-Tryptophan | Absent |
L-Tyrosine•2Na•2H2O | Absent |
L-Valine | Absent |
Vitamins
Choline Chloride | 0.004 |
Folic Acid | 0.004 |
myo-Inositol | 0.0072 |
Niacinamide | 0.004 |
D-Pantothenic Acid•Ca | 0.004 |
Pyridoxal•HCl | 0.004 |
Riboflavin | 0.0004 |
Thiamine•HCl | 0.004 |
Other
D-Glucose | 1 |
Phenol Red, Sodium | 0.0159 |
Pyruvic Acid, Sodium | Absent |
产品组分
组分内容 |
型号 | 规格 |
储存温度 |
DMEM低糖,无氨基酸,丙酮酸(粉) |
D9800-13-10L | 10L | 2-8°C |
DMEM低糖,无氨基酸,丙酮酸(粉) |
D9800-13-25L | 25L | 2-8°C |
DMEM低糖,无氨基酸,丙酮酸(粉) |
D9800-13-50L | 50L | 2-8°C |
DMEM低糖,无氨基酸,丙酮酸(粉) |
D9800-13-100L | 100L | 2-8°C |
操作手册 | 1 | 1 |
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