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RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒
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RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒

RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒,RhoA/Rac1/Cdc42 Activation Assay Combo Biochem Kit (Bead Pull-Down Format) ,非常适合开始WB和SDS-PAGE实验类型。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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检测原理示意图如下(图 1)。 该检测方法基于以下事实:已知 Rho 家族效应蛋白会优先与其目标 GTP 酶的活性(GTP 结合)形式结合(1)。 在 RhoA 激活的情况下,rhotekin-RBD 效应结构域被用来制作亲和珠(2)。在 Cdc42 和 Rac1 激活的情况下,亲和珠使用 PAK-PBD 效应结构域(3)。 Western印迹结果示例和详细方案可点击文件资源下载 pdf 数据表查看。

图1:RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒流程图

RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒具有如下优势和特性:

  • 当实验结果表明一种或多种 Rho 家族蛋白被激活时,组合试剂盒是理想的选择。
  • 提供各10次检测量,分别用于检测活化的 RhoA、Rac1 和 Cdc42。
  • 包括一系列试剂(见下文试剂盒内容)。
  • 获得 Rho 家族活化概况的最经济有效的方法。

References

  1. Aspenstrom, P.  1999.  Effectors for the Rho GTPases.  Curr. Opin. In Cell Biol. 11, 95-102.
  2. Ren, X.D. et al.  1999.  Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton.  EMBO J. 18, 578-585.
  3. Benard, V. et al. 1999. Characterization of Rac and Cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases.  J. Biol. Chem274: 13198-13204 .

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RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒 BK030 30次 2-8°C

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BK030-RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒 操作手册
BK030-RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒相关说明 宣传单页

 

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保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

 

FAQ

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Tiwari, Richa et al. Depletion of keratin 8/18 modulates oncogenic potential by governing multiple signaling pathways FEBS Journal 2018 ISSN 1742-4658
Pan, Yu et al. Dissection of glomerular transcriptional profile in patients with diabetic nephropathy: SRGAP2a protects podocyte structure and function Diabetes 2018 ISSN 1939-327X
Li, Yan et al. Aerobic exercise regulates Rho/cofilin pathways to rescue synaptic loss in aged rats PLoS ONE 2017 ISSN 1932-6203
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Bon, Emeline et al. SCN4B acts as a metastasis-suppressor gene preventing hyperactivation of cell migration in breast cancer Nature Communications 2016 ISSN 2041-1723
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