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RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒
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检测原理示意图如下(图 1)。 该检测方法基于以下事实:已知 Rho 家族效应蛋白会优先与其目标 GTP 酶的活性(GTP 结合)形式结合(1)。 在 RhoA 激活的情况下,rhotekin-RBD 效应结构域被用来制作亲和珠(2)。在 Cdc42 和 Rac1 激活的情况下,亲和珠使用 PAK-PBD 效应结构域(3)。 Western印迹结果示例和详细方案可点击文件资源下载 pdf 数据表查看。
图1:RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒流程图
RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒具有如下优势和特性:
- 当实验结果表明一种或多种 Rho 家族蛋白被激活时,组合试剂盒是理想的选择。
- 提供各10次检测量,分别用于检测活化的 RhoA、Rac1 和 Cdc42。
- 包括一系列试剂(见下文试剂盒内容)。
- 获得 Rho 家族活化概况的最经济有效的方法。
References
- Aspenstrom, P. 1999. Effectors for the Rho GTPases. Curr. Opin. In Cell Biol. 11, 95-102.
- Ren, X.D. et al. 1999. Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 18, 578-585.
- Benard, V. et al. 1999. Characterization of Rac and Cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases. J. Biol. Chem. 274: 13198-13204 .
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内容 | 型号 | 规格 |
储存温度 |
RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒 | BK030 | 30次 | 2-8°C |
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1 | 1 |
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BK030-RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒 | 操作手册 |
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BK030-RhoA/Rac1/Cdc42磁珠激活下拉(Pull-Down)试剂盒相关说明 | 宣传单页 |
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保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
Author | Title | Journal | Year | Article Link |
---|---|---|---|---|
Flores-Muñoz, Carolina et al. | The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal Neurons | Cells | 2022 | ISSN 2073-4409 |
Moreiras, Hugo et al. | Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization | Traffic | 2022 | ISSN 1600--0854 |
Liu, Yao et al. | Exosomes derived from stem cells from apical papilla promote craniofacial soft tissue regeneration by enhancing Cdc42-mediated vascularization | Stem Cell Research and Therapy | 2021 | ISSN 1757-6512 |
Bian, Rui et al. | Research paper rac gtpase activating protein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis | International Journal of Biological Sciences | 2021 | ISSN 1449-2288 |
Qi, Yan et al. | Rhoa/rock pathway activation is regulated by at1 receptor and participates in smooth muscle migration and dedifferentiation via promoting actin cytoskeleton polymerization | International Journal of Molecular Sciences | 2020 | ISSN 1422-0067 |
Frye, Maike et al. | Ephrinb2-ephb4 signalling provides rho-mediated homeostatic control of lymphatic endothelial cell junction integrity | eLife | 2020 | ISSN 2050-084X |
Brisson, Lucie et al. | P2X7 receptor promotes mouse mammary cancer cell invasiveness and tumour progression, and is a target for anticancer treatment | Cancers | 2020 | ISSN 2072-6694 |
Dupraz, Sebastian et al. | RhoA Controls Axon Extension Independent of Specification in the Developing Brain | Current Biology | 2019 | ISSN 0960-9822 |
Birkl, Dorothee et al. | TNFα promotes mucosal wound repair through enhanced platelet activating factor receptor signaling in the epithelium | Mucosal Immunology | 2019 | ISSN 1935-3456 |
Tang, Lian et al. | RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton | Pharmacological Research | 2018 | ISSN 1096-1186 |
Cheng, Lili et al. | Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity | Archives of Toxicology | 2018 | ISSN 1432-0738 |
Tiwari, Richa et al. | Depletion of keratin 8/18 modulates oncogenic potential by governing multiple signaling pathways | FEBS Journal | 2018 | ISSN 1742-4658 |
Pan, Yu et al. | Dissection of glomerular transcriptional profile in patients with diabetic nephropathy: SRGAP2a protects podocyte structure and function | Diabetes | 2018 | ISSN 1939-327X |
Li, Yan et al. | Aerobic exercise regulates Rho/cofilin pathways to rescue synaptic loss in aged rats | PLoS ONE | 2017 | ISSN 1932-6203 |
Majumder, Piyali et al. | Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario | DMM Disease Models and Mechanisms | 2017 | ISSN 1754-8411 |
Bon, Emeline et al. | SCN4B acts as a metastasis-suppressor gene preventing hyperactivation of cell migration in breast cancer | Nature Communications | 2016 | ISSN 2041-1723 |
Zhang, Liang et al. | A lateral signalling pathway coordinates shape volatility during cell migration | Nature Communications | 2016 | ISSN 2041-1723 |
Liu, Chunqiao et al. | A secreted WNT-ligand-binding domain of FZD5 generated by a frameshift mutation causes autosomal dominant coloboma | Human Molecular Genetics | 2016 | ISSN 1460-2083 |
Medapati, Manoj Reddy et al. | RAGE mediates the pro-migratory response of extracellular S100A4 in human thyroid cancer cells | Thyroid | 2015 | ISSN 1557-9077 |
Tang, Jianjun et al. | Paradoxical role of CBX8 in proliferation and metastasis of colorectal cancer | Oncotarget | 2014 | ISSN 1949-2553 |
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