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DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉)
DMEM 是最广泛使用的改良 BME。它所含的氨基酸和维生素的浓度高出 4 倍。添加了非必需氨基酸和某些必需微量元素。增加了碳酸氢盐的浓度。DMEM 的标准配方是 1000 毫克/毫升葡萄糖。DMEM 最初是为培养小鼠胚胎细胞而开发的。如今,它已广泛应用于正常和转化的小鼠和鸡细胞的无血清培养。
Appearance
White, homogeneous, free-flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 10g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as | g/Liter |
Inorganic Salts
Calcium Chloride•2H2O | 0.265 |
Ferric Nitrate•9H2O | 0.0001 |
Magnesium Sulfate | 0.09767 |
Potassium Chloride | 0.4 |
Sodium Chloride | 6.4 |
Sodium Phosphate Monobasic0.109 |
Amino Acids
L-Arginine•HCl | 0.084 |
L-Cystine•2HCl | 0.0626 |
L-Glutamine | 0.584 |
Glycine | 0.03 |
L-Histidine•HCl•H2O | 0.042 |
L-Isoleucine | 0.105 |
L-Leucine | 0.105 |
L-Lysine•HCl | 0.146 |
L-Methionine | 0.03 |
L-Phenylalanine | 0.066 |
L-Serine | 0.042 |
L-Threonine | 0.095 |
L-Tryptophan | 0.016 |
L-Tyrosine•2Na•2H2O | 0.10379 |
L-Valine | 0.094 |
Vitamins
Choline Chloride | 0.004 |
Folic Acid | Absent |
myo-Inositol | 0.0072 |
Niacinamide | Absent |
D-Pantothenic Acid•Ca | 0.004 |
Pyridoxal•HCl | Absent |
Riboflavin | Absent |
Thiamine•HCl | Absent |
Other
D-Glucose | 1 |
Phenol Red, Sodium | Absent |
Pyruvic Acid, Sodium | 0.11 |
产品组分
组分内容 |
型号 | 规格 |
储存温度 |
DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉) |
D9800-12-10L | 10L | 2-8°C |
DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉) |
D9800-12-25L | 25L | 2-8°C |
DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉) |
D9800-12-50L | 50L | 2-8°C |
DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉) |
D9800-12-100L | 100L | 2-8°C |
DMEM低糖,无叶酸,烟酰胺,吡啶酸,维生素B2,苯酚(粉) |
D9800-12-250L | 250L | 2-8°C |
操作手册 | 1 | 1 |
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