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超声法染色质制备试剂盒
染色质免疫沉淀（ChIP）在技术上具有挑战性，并且结果难以解读。我们的酶法染色质免疫沉淀试剂盒通过应用蛋白质G偶联磁珠的简化方案来消除其中的一些挑战，使ChIP只需一天就能完成。试剂盒提供超声或者酶消化两种方案制备染色质。使用我们提供的染色质对照试剂盒和qPCR引物组引物组和已剪切好的染色质来完成您的实验设计，可以在每一步验证您的结果。要了解这些功能与我们提供的其他ChIP试剂盒的差异，请参阅我们的ChIP试剂盒选择指南。
ChIP涉及免疫沉淀经过交联固定的蛋白/DNA复合物。首先，完整的细胞用甲醛固定交联，从而保持蛋白和DNA的相互作用。然后，DNA被剪切成均匀的小片段，使用目标DNA结合蛋白的抗体免疫沉淀DNA/蛋白复合物。免疫沉淀后，DNA经过洗涤，解交联，蛋白被蛋白酶K处理去除。洗脱后的DNA可以用我们的染色体免疫沉淀DNA纯化试剂盒进行纯化，纯化后的DNA可以直接进行qPCR或NGS测序下游分析。
图1：使用酶法染色质免疫沉淀试剂盒示意图可提供高质量的ChIP-Seq数据
使用3µg 我们的AbFlex®组蛋白H3K9ac重组抗体（目录号91103）和的酶法染色质免疫沉淀试剂盒25µg来自K-562细胞的染色质。在ChIP之后，使用下一代DNA文库建库试剂盒（目录号53216和53264）制备Illumina兼容测序文库，并使用NextSeq 500进行测序。结果显示高质量的ChIP-Seq信号峰横跨1号染色体的一个区域。
图2：使用酶法染色质免疫沉淀试剂盒免疫沉淀组蛋白修饰H3K9ac的ChIP-Seq数据 使用酶法染色质免疫沉淀试剂盒富集组蛋白修饰H3K9ac的ChIP qPCR数据
使用3µg 我们的AbFlex®组蛋白修饰H3K9ac重组抗体（货号91103）和酶法染色质免疫沉淀试剂盒免疫沉淀25µg K-562细胞染色质。ChIP后，DNA按1:20稀释，使用我们的人阳性对照引物组ACTB-1和GAPDH-1（货号号71003和71004），人阴性对照引物组1（货号71001）进行qPCR。结果以每1000个细胞检测到的结合情况展示，结果显示阳性对照PCR引物有富集，而阴性对照引物几乎没有富集。
图3：使用酶法染色质免疫沉淀试剂盒免疫沉淀组蛋白修饰H3K9ac的ChIP-qPCR数据
酶法染色质免疫沉淀试剂盒具有如下优势和特性：
- ChIP-IT Express超声法对于难以裂解的细胞、培养细胞和组织样品来说是高质量的染色质片段化方案。
- 如无超声仪，培养细胞系样本只需简单的使用我们的ChIP-IT Express Enzymatic kit。
- 快速的操作步骤使ChIP实验在一天内完成成为可能。
- 蛋白质G磁珠提供低背景富集和简化的洗涤步骤。
产品组分
|
内容 |
型号 |
规格 |
储存温度 |
| 超声法染色质制备试剂盒 | 53032 | 10次 | -20°C |
|
操作手册 |
1 | 1 |
常温 |
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注意事项
| 保存建议 | 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 |
FAQ
“Sulforaphane reactivates cellular antioxidant defense by inducing Nrf2/ARE/Prdx6 activity during aging and oxidative stress.” by Kubo et al. (2017) Scientific Reports 7(14130): 1-17.
“cGAS drives noncanonical-inflammasome activation in age-related macular degeneration.” by Kerur et al. (2018) Nature Medicine 24: 50-61.
“An integrated transcriptome and epigenome analysis identifies a novel candidate gene for pancreatic cancer.” by Jia et al. (2013) BMC Med Genomics 6(33).
“CXCL12 protects pancreatic β-cells from oxidative stress by a Nrf2-induced increase in catalase expression and activity.” by Dinić et al. (2016) Proc Jpn Acad Ser B Phys Biol Sci. 92(9): 436-454.
“Novel computational analysis of protein binding array data identifies direct targets of Nkx2.2 in the pancreas.” by Hill et al. (2011) BMC Bioinformatics 12(62).
“PARP-1 and YY1 are important novel regulators of CXCL12 gene transcription in rat pancreatic beta cells.” by Marković et al. (2013) PLoS ONE 8(3): e59679.
“Synergistic activations of REG I α and REG I β promoters by IL-6 and Glucocorticoids through JAK/STAT pathway in human pancreatic β cells.” by Yamauchi et al. (2015) J Diabetes Res. Epub: 173058.
“Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.” by Hollingworth et al. (2017) J Virol. 91(22): e00930-17.
“Crosstalk between histone modifications indicates that inhibition of arginine methyltransferase CARM1 activity reverses HIV latency.” by Zhang et al. (2017) Nucleic Acids Res 45(16): 9348-9360.
“The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2.” by DeSmet et al. (2016) PLoS Pathogens 12(10): e1005934.
“Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons.” by Rangasamy. (2013) Mob DNA 4(1):27.
“Phosphorylation State of ZFP24 Controls Oligodendrocyte Differentiation.” by Elbaz et al. (2018) Cell Rep 23(8):2254-2263.
“Changes in chromatin state reveal ARNT2 at a node of a tumorigenic transcription factor signature driving glioblastoma cell aggressiveness.” by Bogeas et al. (2018) Acta Neuropathol 135(2):267-283.
“Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex.” by Naik et al. (2018) Transl Psychiatry 8(1):1.
“MELK is a novel therapeutic target in high-risk neuroblastoma.” by Guan et al. (2018) Oncotarget 9(2): 2591–2602.
“The embryonic type of SPP1 transcriptional regulation is re-activated in glioblastoma.” by Kijewska et al. (2018) Oncotarget 8(10):16340-16355.
“BIX01294, an inhibitor of histone methyltransferase, induces autophagy-dependent differentiation of glioma stem-like cells.” by Ciechomska et al. (2016) Scientific Reports 6(38723).
“Enhancing dopaminergic signaling and histone acetylation promotes long-term rescue of deficient fear extinction.” by Whittle et al. (2016) Transl Psychiatry 6(12):e974.
“Tissue mechanics promote IDH1-dependent HIF1α-tenascin C feedback to regulate glioblastoma aggression.” by Miroshnikova et al. (2016) Nat Cell Bio 18(12):1336-1345.
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