您好，欢迎光临艾维缔科技怀来有限公司官方网站！
热线：0313-5935521
搜索
总RNA微量纯化试剂盒
产品描述
产品组分
文件资源
营销中心
注意事项
FAQ
文献追踪
总RNA微量纯化试剂盒可从低细胞数样本中提取总 RNA，并以 20 µL 洗脱液洗脱，方便下游应用。
总RNA微量纯化试剂盒适用于从一系列样本中分离总 RNA，包括少量输入细胞、针刺活检、LCM、CTC 和其他低细胞数样本。提取的 RNA 质量高、纯度高，具有极佳的 RIN 值和 A260/A280，适合 qRT-PCR、RT-PCR、微阵列、NGS 等下游应用。
总RNA微量纯化试剂盒能在同一馏分中纯化所有大小的 RNA，从大 mRNA、lncRNA 到 microRNA (miRNA)，无需使用苯酚。无论大小，都能以相同的速率分离所有 RNA 序列。此外，当 RNA 序列较小时（如 miRNA），无论其 GC 含量如何，色谱柱都能与小 RNA 结合。
|
Kit Specifications |
|
|
Maximum Column Binding Capacity |
35 μg |
|
Maximum Column Loading Volume |
650 μL |
| Minimum Elution Volume |
20 μL |
| Size of RNA Purified |
All sizes, including small RNA |
|
Maximum Amount of Starting Material: Animal Cells Animal Tissues (Brain, Kidney, Liver, Lung, Spleen) Heart, muscle Laser-Captured Microdissection (LCM) |
5 x 105 cells 3 mg (for most tissues*) up to 7.5 mL Up to 5 x 105 cells |
| Time to Complete 10 Purifications |
20 minutes |
|
Average Yield |
|
总RNA微量纯化试剂盒，具有如下的优势和特性：
- 提取高质量和高数量的总 RNA，包括 miRNA
- 无需苯酚步骤--在一个馏分中分离所有 RNA
- 以较小体积（20 µL）洗脱提取的总 RNA
- 结合并洗脱所有 RNA，不论其大小或 GC 含量，无偏差。
- 有效提取小 RNA，无论其 GC 含量如何
- 灵敏度高、线性度好，小至几个细胞，无需载体 RNA
- 方便快捷的自旋柱格式
- 从多种标本中分离
- 纯化的 RNA 适用于各种下游应用，包括小 RNA 测序。
- 纯化基于使用专有树脂分离基质的离心柱色谱法
产品组分
|
组分内容 |
型号 |
规格 |
储存温度 |
| 总RNA微量纯化试剂盒 | 35300 | 50次 | 室温 |
| 总RNA微量纯化试剂盒 | 35350 | 250次 | 室温 |
|
操作手册 |
1 |
1 |
室温 |
文件资源
| 文件下载 | 文件内容 | 资源说明 |
 |
35300-总RNA微量纯化试剂盒 | 操作手册 |
|
35300-总RNA微量纯化试剂盒相关产品 | 宣传单页 |
营销中心
注意事项
| 保存建议 | 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 |
| 警告 | 本品仅供科研使用，请勿用于临床与诊断。 |
FAQ
| Title | Molecular diagnosis of SARS-CoV-2 in seminal fluid |
| Journal | Journal of Endocrinological Investigation. 2021. |
| Authors | D. Paoli, F. Pallotti, G. Nigro, L. Mazzuti, M. N. Hirsch, M. B. Valli, S. Colangelo, C. M. Mastroianni, G. Antonelli, A. Lenzi, O. Turriziani, F. Lombardo |
| Title | Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications |
| Journal | Journal of Biomedical Materials Research. 2017. |
| Authors | Blanquer A, Musilkova J, Barrios L, Ibáñez E, Vandrovcova M, Pellicer E, Sort J, Bacakova L, Nogués C |
| Title | Deficiency in prohormone convertase PC1 impairs prohormone processing in Prader-Willi syndrome |
| Journal | Journal of Clinical Investigation. 2016. |
| Authors | Burnett LC, LeDuc CA, Sulsona CR, Paull D, Rausch R, Eddiry S, Carli JF, Morabito MV, Skowronski AA, Hubner G, Zimmer M, Wang L, Day R, Levy B, Fennoy I, Dubern B, Poitou C, Clement K, Butler MG, Rosenbaum M, Salles JP, Tauber M, Driscoll DJ, Egli D, Leibel RL |
| Title | Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies |
| Journal | International Journal of Molecular Sciences. 2016. |
| Authors | Ma Y, Luk A, Young FP, Lynch D, Chua W, Balakrishnar B, de Souza P, Becker TM |
| Title | Tumor necrosis factor / sphingosine-1-phosphate signaling augments resistance artery myogenic tone in diabetes |
| Journal | Diabetes. 2016. |
| Authors | Sauvé M, Hui SK, Dinh DD, Foltz WD, Momen A, Nedospasov SA, Offermanns S, Husain M, Kroetsch JT, Lidington D, Bolz SS |
| Title | The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering |
| Journal | Int J Nanomedicine. 2016. |
| Authors | Bacakova, M., Musilkova, J., Riedel, T., Stranska, D., Brynda, E., Zaloudkova, M., & Bacakova, L |
| Title | Sphingosine-1-Phosphate Signaling Regulates Myogenic Responsiveness in Human Resistance Arteries |
| Journal | PLOS ONE. 2015. |
| Authors | Sonya Hui, Andrew S. Levy, Daniel L. Slack, Marcus J. Burnstein, Lee Errett, Daniel Bonneau, David Latter, Ori D. Rotstein, Steffen-Sebastian Bolz , Darcy Lidington, Julia Voigtlaender-Bolz |
| Title | Scleraxis is required for the development of a functional tendon enthesis |
| Journal | FASEB Journal. 2015. |
| Authors | Killian ML, Thomopoulos S |
| Title | Cell adhesion and growth enabled by biomimetic oligopeptide modification of a polydopamine-poly(ethylene oxide) protein repulsive surface |
| Journal | Journal of Materials Science: Materials in Medicine. 2015. |
| Authors | Jana Musilkova, Ilya Kotelnikov, Katarina Novotna, Ognen Pop-Georgievski, Frantisek Rypacek, Lucie Bacakova, Vladimir Proks |
| Title | Hypoxia-inducible factor 3-alpha expression is associated with the stable chondrocyte phenotype |
| Journal | Journal of Orthopaedic Research. 2015. |
| Authors | Brandon D. Markway, Holly Cho, Jevgenia Zilberman-Rudenko, Paul Holden, Audrey McAlinden and Brian Johnstone |
| Title | Inhibition of the glucose transporter SGLT2 with dapagliflozin in pancreatic alpha cells triggers glucagon secretion |
| Journal | Nature Medicine. 2015. |
| Authors | Caroline Bonner, Julie Kerr-Conte, Valéry Gmyr, Gurvan Queniat, Ericka Moerman, Julien Thévenet, Cédric Beaucamps, Nathalie Delalleau, Iuliana Popescu, Willy J Malaisse, Abdullah Sener, Benoit Deprez, Amar Abderrahmani, Bart Staels & François Pattou |
| Title | Enthesis fibrocartilage cells originate from a population of Hedgehog-responsive cells modulated by the loading environment |
| Journal | Development. 2015. |
| Authors | Schwartz AG, Long F, Thomopoulos S |
| Title | Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum free embryoid bodies. |
| Journal | American Journal of Physiology - Cell Physiology. 2014. |
| Authors | Michael W. Nestor, Samson Jacob, Bruce Sun, Deborah Pre, Andrew A. Sproul, Seong Im Hong, Chris Woodard, Matthew Zimmer, Vorapin Chinchalongporn, Ottavio Arancio, Scott A. Noggle. |
| Title | Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline. |
| Title | Substance P is associated with hypothalamic paraventricular nucleus activation that coincides with increased urotensin 2 mRNA in chicks. |
| Journal | Neuropeptides. 2014. |
| Authors | Jacob D. Mace, Tetsuya Tachibana, Guoqing Wang, Brandon A. Newmyer, Evin Guilliams, Elizabeth R. Gilbert, Mark A. Cline. |
| Title | Immunoresolving actions of oral resolvin D1 include selective regulation of the transcription machinery in resolution-phase mouse macrophages. |
| Journal | FASEB Journal. 2014. |
| Authors | Recchiuti A, Codagnone M, Pierdomenico AM, Rossi C, Mari VC, Cianci E, Simiele F, Gatta V, Romano M. |
| Title | Differentially Expressed MicroRNAs in Chondrocytes from Distinct Regions of Developing Human Cartilage. |
| Journal | PLoS One. 2013. |
| Authors | McAlinden A, Varghese N, Wirthlin L, Chang LW. |
上一个：
血浆/血清cfc-RNA纯化试剂盒
免责申明:本网站销售的所有产品均不得用于人类或动物之临床诊断或治疗，仅可用于工业或者科研等非医疗目的。(获得国家相关部门批准的产品除外)
版权所有：艾维缔科技怀来有限公司 备案号：冀ICP备20011415号
