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5-羟甲基胞嘧啶（5-hmC）作为第六种DNA碱基，具有转录调节的功能，已被检测到在人类和小鼠大脑和胚胎干细胞（ES）中含量丰富。在哺乳动物中，它可以通过5-mC的氧化产生，这一反应由10-eleven translocation（TET）家族的5-mC-羟化酶介导。5-hmC已被证明具有组织特异性，从培养的细胞系中检测不到的水平到人脑组织中的0.6%，在一些其他物种中可高达总DNA的8%。5-hmC作为表型和基因表达的重要表观遗传学修饰，其生物学意义最近已被确认。例如，5-hmC含量的全面下降（DNA低甲基化）在几乎所有的癌症中都有表现，并被提议作为癌症的分子标记和治疗目标。
总体DNA羟甲基极易定量检测试剂盒（比色法）中含有定量检测全基因组羟甲基化所必需的所有试剂。在检测实验中，DNA被固定在一种有DNA吸附能力的孔上，甲基化的片段会被捕获剂以及检测抗体识别，用酶标仪读取在450 nm处的OD值来定量。羟甲基化的DNA的量与OD值成线性关系，可以通过本试剂盒提供的方程来计算两种不同的甲基化DNA样品的相对甲基化状态或者通过标准曲线完全定量5-hmc。基于其工作原理和微孔板模式，总体DNA羟甲基化极易定量检测试剂盒（比色法）可以用于各种物种来源的多种样品形式，包括培养的细胞、新鲜和冷冻的组织、石蜡包埋组织、血浆/血清样品以及体液样品。
总体DNA羟甲基化极易定量检测试剂盒（比色法）具有如下优点和特性：
- 快速：减少的步骤，整个操作仅需要2小时；
- 稳定性：优化的试剂盒组分，允许在做复孔时，有更大的“触发信号”，尽量减少变化；
- 方便性：固定的低背景噪音，从而消除了DNA变性和板孔的限定性；
- 灵敏度：检测下限低至0.01%的羟甲基化DNA，输入量在100ng；
- 特异性：高效特异结合5-hmC，在复孔的浓度范围内，对于未甲基化的胞嘧啶和羟甲基的胞嘧啶无特异性反应，仅对羟甲基化DNA（5-hmC）进行高度专一的检测;
- 通用型：阳性与阴性对照，对于任何种属的羟甲基化DNA状态进行定量检测是非常理想的；
- 精确性：标准的阳性对照可以得到标准曲线，可以与HPLC-MS相媲美，用时短，效率高，。
- 灵活性：微孔板（96孔）的设计使实验更灵活，无论对于手动还是高通量分析简单、可信和检测实验条件恒始终如一。
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产品组分
组分内容
型号：A-P-1032-48(48次)
型号：A-P-1032-96(96次) 储存温度
WB
10X Wash Buffer
14 ml
28 ml
4°C BS
Binding Solution
5 ml 10 ml 室温 NC
Negative Control,0% 5-hmC，50ug/ml *
50 ul
100 ul
-20°C PC
Positive Control,1% 5-hmC，50ug/ml *
10 ul
20 ul
-20°C hmAb
5-hmC Antibody,1000X*
5 ul 10 ul 4°C SI
Signal Indicator,1000X*
5 ul 10 ul -20°C ES
Enhancer Solution,1000X*
5 ul 10 ul -20°C DS
Developer Solution
5 ml 10 ml 4°C SS
Stop Solution
5 ml
10 ml
4°C 8-联管
8-Well Assay Strips(With Frame)
6
12
4°C
操作手册
1
1 室温
*注意：在使用各溶液之前，将溶液离心至管底。
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保存建议 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 警告 本品仅供科研使用，请勿用于临床与诊断。 -
FAQ
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