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HRP标记抗小鼠Ig单克隆抗体
HRP标记抗小鼠Ig单克隆抗体是一种独特的抗小鼠 IgG 单克隆二抗。小鼠 IgG TrueBlot® ULTRA 可检测免疫印迹的目标蛋白条带,不受干扰免疫沉淀免疫球蛋白重链和轻链的阻碍。只需用小鼠 IgG TrueBlot® ULTRA 取代传统的抗小鼠 IgG 印迹试剂,并按照规定的方案进行样品制备和免疫印迹,就能使用小鼠 IgG TrueBlot® ULTRA 轻松生成出版物质量的 IP/Western 印迹数据。小鼠 IgG TrueBlot® ULTRA 非常适合用于免疫沉淀蛋白的免疫印迹。小鼠 IgG TrueBlot® ULTRA 可优先检测未还原的小鼠 IgG(IgG1、IgG2a、IgG2b、IgG3),而不是还原的、SDS 变性的 IgG。当免疫沉淀在 SDS-凝胶电泳前被完全还原时,小鼠 IgG TrueBlot® ULTRA 与免疫沉淀抗体的 55 kDa 重链和 23 kDa 轻链的反应性将降至最低,从而消除了免疫沉淀抗体重链和轻链在 IP/免疫印迹应用中的干扰。其应用包括研究翻译后修饰(如磷酸化或乙酰化)或蛋白质与蛋白质之间的相互作用。
ELISA, IP, WB
Biochemical Assay, ChIP, IF
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP
Anti-Mouse IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Rat
Peroxidase (Horseradish) ULTRA
Monoclonal
IgG
Mouse
Mouse TrueBlot® ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
Liquid (sterile filtered)
1.0 mg/mL
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Proclin is added as an antimicrobial agent.
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
产品组分
内容 |
型号 |
规格 |
储存温度 |
HRP标记抗小鼠Ig单克隆抗体 | CN18-8817-01 | 20ul | -20°C |
HRP标记抗小鼠Ig单克隆抗体 | CN18-8817-02 | 50ul | -20°C |
HRP标记抗小鼠Ig单克隆抗体 | CN18-8817-03 | 50ul | -20°C |
操作手册 |
1 | 1 |
常温 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
Surana S et al. (2023). The tyrosine phosphatase LAR acts as a receptor of the nidogen-tetanus toxin complex. bioRxiv Preprint
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Jin D et al. (2023). α2δ‐1 protein drives opioid‐induced conditioned reward and synaptic NMDA receptor hyperactivity in the nucleus accumbens. J Neurochem.
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Pappas G et al. (2023). MDC1 maintains active elongation complexes of RNA polymerase II. Cell Rep.
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Gong L et al. (2023). AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex. Am J Repair Cell Mol Biol.
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Boehm D et al. (2023). The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell Rep.
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Refaat AM et al. (2023). HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression. Cell Rep.
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Chauhan C et al. (2023). PRMT5-mediated regulatory arginine methylation of RIPK3. Cell Death Discov.
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Malgorzata Bodaszewska-Lubas et al. (2022). Dominant-Negative Form of SIGIRR: SIGIRRΔE8 Promotes Tumor Growth Through Regulation of Metabolic Pathways. J Interferon Cytokine Res.
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Aso M et al. (2022). First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency. Heliyon.
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Blue SM et al. (2022). Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq. Nat Protoc.
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Amar-Schwartz A et al. (2022). S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. Elife.
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Janneh AH et al. (2022). Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis. Cell Rep.
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Park HJ et al. (2022). Anti‑inflammatory effects of Nypa fruticans Wurmb via NF‑κB and MAPK signaling pathways in macrophages. Exp Ther Med.
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Alerasool N et al. (2022). Identification and functional characterization of transcriptional activators in human cells. Mol Cel.
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Shen JZ et al. (2022). A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell.
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Liang G et al. (2022). CTNNBL1 restricts HIV-1 replication by suppressing viral DNA integration into the cell genome. Cell Rep.
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Cha SJ et al. (2022). Therapeutic modulation of GSTO activity rescues FUS-associated neurotoxicity via deglutathionylation in ALS disease models. Dev Cell.
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Lahree A et al. (2022). Active APPL1 sequestration by Plasmodium favors liver-stage development. Cell Rep.
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Huang X et al. (2022). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep.
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IP, Co-IP
Zeng LW et al. (2022). The membrane-associated ubiquitin ligases MARCH2 and MARCH3 target IL-5 receptor alpha to negatively regulate eosinophilic airway inflammation. Cell Mol Immunol.
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Volkmar N et al. (2022). Regulation of membrane fluidity by RNF145‐triggered degradation of the lipid hydrolase ADIPOR2. EMBO J.
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Desjardins P et al. (2022). The WNK1 kinase regulates the stability of transcription factors during wound healing of human corneal epithelial cells. J Cell Physiol.
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Namba Y et al. (2022). Maelstrom functions in the production of Siwi-piRISC capable of regulating transposons in Bombyx germ cells. iScience.
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Sarodaya N et al. (2022). Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity. Cell Biol Toxicol.
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Saha B et al. (2022). Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep.
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