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488荧光原位杂交(FISH)成像试剂盒
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488荧光原位杂交(FISH)成像试剂盒

488荧光原位杂交(FISH)成像试剂盒,ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit,是使用488 标记的 FISH 探针通过原位杂交标记靶 DNA 的便捷工具。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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荧光原位杂交(FISH)技术是检测生物标本中特定核酸靶点的有效工具。通过荧光染料标记的核酸探针与标本的互补序列杂交,可实现核酸靶标的原位检测。488荧光原位杂交(FISH)成像试剂盒是使用 iFluor® 488 标记的 FISH 探针通过原位杂交标记靶 DNA 的便捷工具。该试剂盒提供 Taq DNA 聚合酶,通过聚合酶链式反应 (PCR) 将 iFluor® 488-dUTPs 结合到目标 DNA 中。我们专有的 iFluor® 染料比传统的荧光标签更亮、更耐光照,能提供所需的分辨率和信号。

图 1. 使用 iFluor® 488-dUTP 标记的端粒探针对分裂期 HeLa 细胞进行端粒定量荧光原位杂交。探针使用488荧光原位杂交(FISH)成像试剂盒制作。

操作步骤

使用前,将所有组分解冻至室温,涡旋充分混合。

注:以下方案可作为标准DNA FISH的通用指南。请根据自己的实验进行方案的修改或优化。

1.配制如下反应混合液,见表1

每个反应每孔的试剂组成

成分 体积 (25 µL/反应) 最终浓度
FISH反应混合物 (2X) 12.5 µL 1X
上游引物, 10 µM 0.25-2.5 µL 0.1-1.0 µM
下游引物, 10 µM 0.25-2.5 µL 0.1-1.0 µM
模板DNA 1-5 µL 优化浓度
iFluor 488-dUTP 2.5 µL  
dNTP 混合物 1 µL  
水,无核酸酶 25 µL  

2.通过缓速涡旋,然后用短暂的离心机小心地混合试剂

3.在qPCR仪中置板,按下表2操作

表2 .热循环参数

参数

聚合酶活化

PCR (30-40 个周期)

 

持续温度

变性

退火

延长

温度

95 °C

95 °C

55-65 °C

68-72 °C

时间 (m:ss)

0:20

0:30

1:00

1:00

产品组分

内容

型号

规格

储存温度

488荧光原位杂交(FISH)成像试剂盒 CN17310-01 25次 -20°C
488荧光原位杂交(FISH)成像试剂盒 CN17310-02 50次 -20°C

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1 1

常温

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CN17310-488荧光原位杂交(FISH)成像试剂盒 操作手册
CN17310-488荧光原位杂交(FISH)成像试剂盒相关单页 宣传单页

 

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保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

 

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