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组蛋白H3K4me3多克隆抗体
Application: | ChIP, ChIP-seq, DB, WB |
Areas of Research: | Histone Methylation & Demethylation |
Clonality: | Polyclonal |
Conjugate: | Unconjugated |
Host: | Rabbit |
Isotype: | IgG |
Purification: | Affinity Purified |
Reactivity: | Broad Range, Human, Mouse, Rat |
Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
Description
Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Specificity
Broad Range, Mouse, Rat, Human
Isotype
IgG
Uniprot ID
Q16695
Purification
Affinity Purified
Immunogen
A synthetic trimethylated peptide around K4 of human histone H3 (NP_003520.1)
Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.
Alternative Names
H3K4me3 antibody, H3K4m3 antibody
Application
DB, WB, ChIP, ChIP-seq; Recommended dilution, DB 1:500 - 1:2000, WB 1:500 - 1:2000, ChIP 1:20 - 1:100, ChIP-seq 1:20 - 1:100
产品组分
内容 |
型号 |
规格 |
储存温度 |
组蛋白H3K4me3多克隆抗体 | A-4033-025 | 25ul | -20°C |
组蛋白H3K4me3多克隆抗体 | A-4033-050 | 50ul | -20°C |
组蛋白H3K4me3多克隆抗体 | A-4033-100 | 100ul | -20°C |
操作手册 |
1 | 1 |
常温 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
Sajjanar B et. al. (August 2019). Cross-talk between energy metabolism and epigenetics during temperature stress response in C2C12 myoblasts. Int J Hyperthermia. 36(1):776-784.
Lin J et. al. (June 2016). The relationship between apoptosis, chromatin configuration, histone modification and competence of oocytes: A study using the mouse ovary-holding stress model. Sci Rep. 6:28347.
Eid A et. al. (February 2016). Developmental lead exposure and lifespan alterations in epigenetic regulators and their correspondence to biomarkers of Alzheimer's disease Alzheimer's and Dement. :43839.
Jacques-Fricke BT et. al. (December 2014). Neural crest specification and migration independently require NSD3-related lysine methyltransferase activity. Mol Biol Cell. 25(25):4174-86.
Tong Z et. al. (July 2014). Age-related formaldehyde interferes with DNA methyltransferase function, causing memory loss in Alzheimer's disease. Neurobiol Aging.
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