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William's E培养基粉末(含L-谷氨酰胺)
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William's E培养基粉末(含L-谷氨酰胺)

William's E培养基粉末(含L-谷氨酰胺),Williams’ Medium E, w/L-Glutamine (Powder)。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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Appearance

Light orange, homogeneous, free flowing powder

Solubility

Yellow, clear, complete

pH

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 10.8g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Chloride•2H2O 0.265
Cupric Sulfate•H2O 0.0000001
Ferric Nitrate•9H2O 0.0000001
Magnesium Sulfate 0.0977
Manganese Chloride•4H2O 0.0000001
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Monobasic 0.122
Zinc Sulfate•7H2O 0.0000002

Amino Acids

L-Alanine 0.09
L-Arginine 0.05
L-Aspartic Acid 0.03
L-Asparagine•H2O 0.02
L-Cysteine (free acid) 0.04
L-Cystine•2HCl 0.02
L-Glutamic Acid 0.0445
L-Glutamine 0.292
Glycine 0.05
L-Histidine 0.015
L-Isoleucine 0.05
L-Leucine 0.075
L-Lysine•HCl 0.08746
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.03
L-Serine 0.01
L-Threonine 0.04
L-Tryptophan 0.01
L-Tyrosine•2Na•2H2O 0.05045
L-Valine 0.05

Vitamins

Ascorbic Acid•Na 0.00227
D-Biotin 0.0005
Choline Chloride 0.0015
Vitamin B-12 0.0002
Calciferol 0.0001
Folic Acid 0.001
myo-Inositol 0.002
Menadione (sodium bisulfate) 0.00001
Niacinamide 0.001
D-Pantothenic Acid•Ca 0.001
Pyridoxal•HCl 0.001
Riboflavin 0.0001
Thiamine•HCl 0.001
DL-a-Tocopherol Phosphate•2Na 0.00001
Retinol Acetate 0.0001

Other

D-Glucose 2.00
Glutathione 0.00005
Methyl Linoleate 0.00003
Phenol Red, Sodium 0.0107
Pyruvic Acid, Sodium 0.025
Total: 10.8g/L

产品组分

组分内容

型号

规格

储存温度

William's E培养基粉末(含L-谷氨酰胺)

W1105-01

10L 4°C

William's E培养基粉末(含L-谷氨酰胺)

W1105-02

25L 4°C

William's E培养基粉末(含L-谷氨酰胺)

W1105-03

50L 4°C

William's E培养基粉末(含L-谷氨酰胺)

W1105-04

100L 4°C

William's E培养基粉末(含L-谷氨酰胺)

W1105-05

250L 4°C
操作手册 1  1

常温

    

文件资源

文件下载 文件内容 资源说明
W1105-William's E培养基粉末(含L-谷氨酰胺) 操作手册
W1105-William's E培养基粉末(含L-谷氨酰胺)相关说明 宣传单页

营销中心1

注意事项

保存建议 推荐蓝冰或冰袋运输。当您收到产品后,按照说明书建议保存各组分。

 

FAQ

References

1. Williams, G.M. and Gunn, J.M. Exp. Cell Research 89:39-142 (1974) 2. Williams, G.M., Weisburger, E.K. and Weisburger, J.H., Exp. Cell Research 69:106-112 (1971)

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