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RhoA激活分析试剂盒(荧光法)
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这种RhoA激活分析试剂盒(荧光法)可测量细胞中 GTP 负载的 RhoA 水平。活化水平通过荧光测定法测量。G-LISA Rho 活化检测试剂盒是基于 ELISA 的 Rho 活化检测试剂盒,可在 3 小时内测定细胞中的 Rho 活性。有关RhoA激活分析试剂盒(荧光法)的更详细介绍以及其他可用的 G-LISA™ 试剂盒列表,请参阅我们的 相关页面。如需使用比色法检测 RhoA 活化的试剂盒,请参阅型号:CNBK124。
图 1. 用RhoA激活分析试剂盒(荧光法)测定溶血磷脂酸(LPA)对 Rho 的激活作用。对瑞士 3T3(小鼠)、A431(人类)和 HeLa(人类)细胞进行血清饥饿处理,然后用 LPA 进行刺激。 对 25 µg 的裂解液进行 G-LISA™ 检测。 所示数据为相对于背景信号(仅用裂解缓冲液而非细胞裂解液培养的孔)的相对发光单位 (RLU)。 LPA 条形上方的数字对应的是与血清饥饿对照样本相比的激活倍数。
RhoA激活分析试剂盒(荧光法)具有如下优势和特性:
- Rho 信号通路研究
- 使用原代细胞进行 Rho 激活试验
- Rho 激活剂和失活剂研究
- 利用有限材料进行 Rho 激活试验
- 高通量筛选 Rho 激活
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规格 |
储存温度 |
RhoA激活分析试剂盒(荧光法) | BK121 | 96次 | 2-8°C |
操作手册 |
1 | 1 |
常温 |
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保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
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Author | Title | Journal | Year | Article Link |
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Wang, Li et al. | Essential role of obscurin kinase-1 in cardiomyocyte coupling via N-cadherin phosphorylation | JCI Insight | 2024 | ISSN 0021--9738 |
Xiao, Liyan et al. | Amorphous silica nanoparticles cause abnormal cytokinesis and multinucleation through dysfunction of the centralspindlin complex and microfilaments | Particle and Fibre Toxicology | 2023 | ISSN 1743-8977 |
He, Yi et al. | CdGAP is a talin-binding protein and a target of TGF-β signaling that promotes HER2-positive breast cancer growth and metastasis | Cell reports | 2023 | ISSN 2211--1247 |
Simonetti, Manuela et al. | The impact of Semaphorin 4C/Plexin-B2 signaling on fear memory via remodeling of neuronal and synaptic morphology | Molecular Psychiatry | 2021 | ISSN 1476-5578 |
Lee, Sang Joon et al. | AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence | Nature | 2021 | ISSN 1476-4687 |
Talamás-Lara, Daniel et al. | Entamoeba histolytica and Entamoeba dispar: Morphological and Behavioral Differences Induced by Fibronectin through GTPases Activation and Actin-Binding Proteins | The Journal of eukaryotic microbiology | 2020 | ISSN 1550--7408 |
Algaber, Anwar et al. | MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA | Scientific Reports 2020 10:1 | 2020 | ISSN 2045--2322 |
dos Santos, Marlus Alves et al. | Human B cells infected by Trypanosoma cruzi undergo F-actin disruption and cell death via caspase-7 activation and cleavage of phospholipase Cγ1 | Immunobiology | 2020 | ISSN 1878--3279 |
Elshaer, Sally L. et al. | Modulation of the p75 neurotrophin receptor using LM11A-31 prevents diabetes-induced retinal vascular permeability in mice via inhibition of inflammation and the RhoA kinase pathway | Diabetologia | 2019 | ISSN 1432-0428 |
Wu, Xuping et al. | Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells | Cancer Management and Research | 2019 | ISSN 1179-1322 |
Mei, Jie et al. | A DAAM1 3′-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis | Cancer Cell International | 2019 | ISSN 1475-2867 |
Yan, Ting et al. | Integrin αvβ3-associated DAAM1 is essential for collagen-induced invadopodia extension and cell haptotaxis in breast cancer cells | Journal of Biological Chemistry | 2018 | ISSN 1083-351X |
Alizadeh, Javad et al. | Detection of small gtpase prenylation and gtp binding using membrane fractionation and gtpase-linked immunosorbent assay | Journal of Visualized Experiments | 2018 | ISSN 1940-087X |
López-Contreras, Luilli et al. | Structural and functional characterization of the divergent Entamoeba Src using Src inhibitor-1 | Parasites and Vectors | 2017 | ISSN 1756-3305 |
Paldy, Eszter et al. | Semaphorin 4C Plexin-B2 signaling in peripheral sensory neurons is pronociceptive in a model of inflammatory pain | Nature Communications | 2017 | ISSN 2041-1723 |
Ruggiero, Carmen et al. | Dosage-dependent regulation of VAV2 expression by steroidogenic factor-1 drives adrenocortical carcinoma cell invasion | Science Signaling | 2017 | ISSN 1937-9145 |
Kim, Jongshin et al. | YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation | Journal of Clinical Investigation | 2017 | ISSN 1558-8238 |
Itano, Seiji et al. | Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model | Molecular Medicine Reports | 2017 | ISSN 1791-3004 |
Nour-Eldine, Wared et al. | Adiponectin attenuates angiotensin II-induced vascular smooth muscle cell remodeling through nitric oxide and the RhoA/ROCK pathway | Frontiers in Pharmacology | 2016 | ISSN 1663-9812 |
Herrera-Martínez, Mayra et al. | Antiamoebic activity of Adenophyllum aurantium (L.) strother and its effect on the actin cytoskeleton of Entamoeba histolytica | Frontiers in Pharmacology | 2016 | ISSN 1663-9812 |
Park, Yong Hwan et al. | Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS | Nature Immunology | 2016 | ISSN 1529-2916 |
Ajima, Rieko et al. | DAAM1 and DAAM2 are co-required for myocardial maturation and sarcomere assembly | Developmental Biology | 2015 | ISSN 1095-564X |
Yan, Yi et al. | Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5 | Nature Communications | 2015 | ISSN 2041-1723 |
Nakajima, Chikako et al. | The lipoprotein receptor LRP1 modulates sphingosine-1-phosphate signaling and is essential for vascular development | Development (Cambridge) | 2014 | ISSN 1477-9129 |
Lasgorceix, M. et al. | In vitro and in vivo evaluation of silicated hydroxyapatite and impact of insulin adsorption | Journal of Materials Science: Materials in Medicine | 2014 | ISSN 1573-4838 |
Moniz, Sónia et al. | Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness | Human molecular genetics | 2013 | ISSN 1460--2083 |
Bray, Kristi et al. | Cdc42 overexpression induces hyperbranching in the developing mammary gland by enhancing cell migration | Breast Cancer Research | 2013 | ISSN 1465-5411 |
Veeramah, Krishna R. et al. | Exome sequencing reveals new causal mutations in children with epileptic encephalopathies | Epilepsia | 2013 | ISSN 0013-9580 |
Bai, Xue et al. | The smooth muscle-selective RhoGAP GRAF3 is a critical regulator of vascular tone and hypertension | Nature Communications | 2013 | ISSN 2041-1723 |
Naraoka, Masato et al. | Suppression of the Rho/Rho-Kinase Pathway and Prevention of Cerebral Vasospasm by Combination Treatment with Statin and Fasudil After Subarachnoid Hemorrhage in Rabbit | Translational Stroke Research | 2013 | ISSN 1868-4483 |
Herrera-Martínez, M. et al. | Actin, RhoA, and Rab11 participation during encystment in entamoeba invadens | BioMed Research International | 2013 | ISSN 2314-6133 |
Nobe, Koji et al. | Two distinct dysfunctions in diabetic mouse mesenteric artery contraction are caused by changes in the Rho A-Rho kinase signaling pathway | European journal of pharmacology | 2012 | ISSN 1879--0712 |
Zuo, Yufeng et al. | Cdc42 negatively regulates intrinsic migration of highly aggressive breast cancer cells | Journal of cellular physiology | 2012 | ISSN 1097--4652 |
Wang, Jiping et al. | RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response | Cardiovascular Diabetology | 2012 | ISSN 1475-2840 |
Doherty, Jason T. et al. | Skeletal muscle differentiation and fusion are regulated by the BAR-containing Rho-GTPase-activating Protein (Rho-GAP), GRAF | Journal of Biological Chemistry | 2011 | ISSN 0021-9258 |
Alvarez, S. M. et al. | Failure of Bay K 8644 to induce RhoA kinase-dependent calcium sensitization in rabbit blood vessels | British journal of pharmacology | 2010 | ISSN 1476--5381 |
Hammar, Eva et al. | Role of the Rho-ROCK (Rho-Associated Kinase) Signaling Pathway in the Regulation of Pancreatic β-Cell Function | Endocrinology | 2009 | ISSN 0013--7227 |
Heckman-Stoddard, Brandy M. et al. | Haploinsufficiency for p190B RhoGAP inhibits MMTV-Neu tumor progression | 2009 | ISSN 1465-5411 | |
Chastre, Eric et al. | TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness | The FASEB Journal | 2009 | ISSN 1530--6860 |
Moniz, Sónia et al. | WNK2 modulates MEK1 activity through the Rho GTPase pathway | Cellular signalling | 2008 | ISSN 0898--6568 |
Kinoshita, Nagatoki et al. | Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation | Molecular Biology of the Cell | 2008 | ISSN 1059-1524 |
Iesato, Ken et al. | Tiotropium bromide attenuates respiratory syncytial virus replication in epithelial cells | Respiration; international review of thoracic diseases | 2008 | ISSN 1423--0356 |
Tanaka, Shigeru et al. | Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth | The Journal of biological chemistry | 2007 | ISSN 0021--9258 |
Schreibelt, Gerty et al. | Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 2007 | ISSN 1530--6860 |
Scott, Glynis A. et al. | Lysophosphatidylcholine mediates melanocyte dendricity through PKCzeta activation | The Journal of investigative dermatology | 2007 | ISSN 1523--1747 |
Higashibata, Akira et al. | Influence of simulated microgravity on the activation of the small GTPase Rho involved in cytoskeletal formation - Molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor | BMC Biochemistry | 2006 | ISSN 1471-2091 |
Woods, Anita et al. | RhoA/ROCK Signaling Regulates Chondrogenesis in a Context-dependent Manner * | Journal of Biological Chemistry | 2006 | ISSN 0021--9258 |
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