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羟基丁酸(酮体)测定分析试剂盒(比色法)
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羟基丁酸(酮体)测定分析试剂盒(比色法)

羟基丁酸(酮体)测定分析试剂盒(比色法)又名:β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit,是一种快速的检测方法,可用于从血浆、血清、尿液、细胞裂解物和组织匀浆中测量β-HB。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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β羟基丁酸(βhb; 3-羟基丁酸(3-羟基丁酸)是一种“酮体”,主要由脂肪酸氧化产生,输出到外周组织作为能量来源。 “酮体”一词指的是三种分子:乙酰乙酸、β-HB和丙酮。 β-HB和乙酰乙酸将能量从肝脏输送到其他组织,而丙酮是由乙酰乙酸自发脱羧产生的 酮症可能是正常的,也可能是病理性的。 正常情况下,酮症可以表明脂质代谢已经被激活,脂质降解途径是完整的。 正常的酮症在许多情况下都很普遍,比如在禁食期间、长时间运动后或在高脂肪饮食后。 酮症的病理原因包括多器官功能衰竭、糖尿病、儿童期低血糖、皮质类固醇或生长激素缺乏症、酒精或水杨酸中毒以及一些先天代谢错误 在急性病人中,这些酮体可在体内积累,导致酮症酸中毒,从而导致潜在的危及生命的情况,即代谢性酸中毒 酮症的存在和程度可以通过测量血液中β-HB的水平来确定。 通常,β-HB约占血清酮体的75%。 4,5,6测量β-HB为酮症酸中毒水平提供了可靠的指标,包括亚临床酮症的检测。 7,8,9在糖尿病患者中,β-HB测量(和血糖)可以用来评估糖尿病昏迷的严重程度,并且对排除高渗性非酮症糖尿病昏迷至关重要。 根据现有的高酮血症,测量β-HB也用于监测胰岛素需求。 β-HB最近被用于神经退行性疾病和抑制脂肪细胞脂分解。 11,12,13,14,15 Cayman 's β-HB(酮体)检测试剂盒提供了一个简单,重复性和敏感性的工具,用于测量血浆,血清,尿液,细胞裂解液或组织匀浆中的β-HB水平。 β-HB的测定方法是以3-羟基丁酸脱氢酶将d -3-羟基丁酸氧化为乙酰乙酸为基础的 伴随着这种氧化,辅助因子NAD+被还原为NADH。 在diaphorase的存在下,NADH与比色检测器WST-1反应产生福尔马赞染料,在445-455 nm的吸光度最大。 染料的吸光度与β-HB浓度成正比。

羟基丁酸(酮体)测定分析试剂盒(比色法)具有如下特性: 
1.从血浆、血清、尿液、细胞裂解物和组织匀浆中测量β-HB

2.化验40个样品,一式二份  
3.测量范围25~500μM 
4.平板比色法(445-455 nm) 

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羟基丁酸(酮体)测定分析试剂盒(比色法) 700190 96次 2~8°C

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1 1

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保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

 

FAQ

1. Guthrie, J.P., and Jordan, F. Amine-catalyzed decarboxylation of acetoacetic acid. The rate constant for decarboxylation of a β-imino acid. Journal of the American Chemical Society 94(26), 9136-9141 (1972).

2. Galán, A., Hernández, J.M., and Jimenez, O. Measurement of blood acetoacetate and β-hydroxybutyrate in an automatic analyser. Journal of Automated Methods & Management in Chemistry 23(3), 69-76 (2001).

3. Foster, D.W., and McGarry, J.D. The metabolic derangements and treatment of diabetic ketoacidosis. New England Journal of Medicine 309(3), 159-169 (1983).

4. Persson, B. Determination of plasma acetoacetate and D-ß-hydroxy-butyrate in new-born infants by an enzymatic fluorometric micro-method. Scandinavian Journal of Clinical and Laboratory Investigation 25(1), 9-18 (1970).

5. Wildenhoff, K.E. A micro-method for the enzymatic determination of acetoacetate and 3-hydroxybutyrate in blood and urine. Scandinavian Journal of Clinical and Laboratory Investigation 25(2), 171-179 (1970).

6. Koch, D.D., and Feidbruegge, H. Optimized kinetic method for automated determination of β-hydroxybutyrate. Clinical Chemistry 33(10), 1761-1766 (1987).

7. Li, P.K., Lee, J.T., MacGillivray, M.H., et al. Direct, fixed-time kinetic assays for β-hydroxybutyrate and acetoacetate with a centrifugal analyzer or a computer-backed spectrophotometer. Clinical Chemistry 26(12), 1713-1717 (1980).

8. Harano, Y., Kosugi, K., Hyosu, T., et al. Sensitive and simplified method for the differential determination of serum levels of ketone bodies. Clinica Chimica Acta 134, 327-336 (1983).

9. MacGillivray, M.H., Li, P.K., Lee, J.T., et al. Elevated plasma ß-hydroxybutyrate concentrations without ketonuria in healthy insulin-dependent diabetic patients. Journal of Clinical Endocrinology and Metabolism 54(3), 665-668 (1982).

10. Alberti, K.G.M.M., and Hockaday, T.D.R. Rapid blood ketone body estimation in the diagnosis of diabetic ketoacidosis. British Medical Journal 2, 565-568 (1972).

11. Kashiwaya, Y., Takeshima, T., Mori, N., et al. D-β-hydroxybutyrate protects neurons in models of Alzheimer’s and Parkinson’s disease. Proceedings of the National Academy of Sciences of the United States of America 97(10), 5440-5444 (2000).

12. Tieu, K., Perier, C., Caspersen, C., et al. D-β-hydroxybutyrate rescues mitochondrial respiration and mitigates features of Parkinson disease. Journal of Clinical Investigation 112(6), 892-901 (2003).

13. Reger, M.A., Henderson, S.T., Hale, C., et al. Effects of β-hydroxybutyrate on cognition in memory-impaired adults. Neurobiology of Aging 25, 311-314 (2004).

14. Cheng, B., Yang, X., Hou, Z., et al. D-β-hydroxybutyrate inhibits the apoptosis of PC12 cells induced by 6-OHDA in relation to up-regulating the ratio of Bcl-2/Bax mRNA. Autonomic Neuroscience: Basic and Clinical 134, 38-44 (2007).

15. Taggart, A.K.P., Kero, J., Gan, X., et al. (D)-β-hydroxybutyrate inhibits adipocyte lipolysis via the nicotinic acid receptor PUMA-G. The Journal of Biological Chemisty 280(29), 26649-26652 (2005).

16. McMurray, C.H., Blanchflower, W.J., and Rice, D.A. Automated kinetic method for D-3-hydroxybutyrate in plasma or serum. Clinical Chemistry 30(3), 421-425 (1984).

Product Citations

Patil, I., Sancheti, H., Stiles, B.L., et al. Brain metabolic and functional alterations in a liver-specific PTEN knockout mouse model. PLoS One 13(9), e0204043 (2018).

Harun-Or-Rashid, M., Pappenhagen, N., Palmer, P.G., et al. Structural and functional rescue of chronic metabolically stressed optic nerves through respiration. J. Neurosci. 38(22), 5122-5139 (2018).

Luo, W., Qin, L., Li, B., et al. Inactivation of HMGCL promotes proliferation and metastasis of nasopharyngeal carcinoma by suppressing oxidative stress. Sci. Rep. 7(1), 11954 (2017).

Kephart, W.C., Mumford, P.W., Mao, X., et al. The 1-week and 8-month effects of a ketogenic diet or ketone salt supplementation on multi-organ markers of oxidative stress and mitochondrial function in rats. Nutrients 9(9), e1019 (2017).

Wang, A., Huen, S.C., Luan, H.H., et al. Opposing effects of fasting metabolism on tissue tolerance in bacterial and viral inflammation. Cell 166(6), 1512-1525 (2016).

Geisler, C.E., Hepler, C., Higgins, M.R., et al. Hepatic adaptations to maintain metabolic homeostasis in response to fasting and refeeding in mice. Nutr. Metab. (Lond.) 13, 62 (2016).

French, R.P., Lyle, J., Tracey, S., et al. High survivorship after catch-and-release fishing suggests physiological resilience in the endothermic shortfin mako shark (Isurus oxyrinchus). Conserv. Physiol. 3(1), cov044 (2015).

Allalou, A., Nalla, A., Prentice, K.J., et al. A predictive metabolic signature for the transition from gestational diabetes mellitus to type 2 diabetes. Diabetes 65(9), 2529-2539 (2016).

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