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组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)
组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)是一套方便的工具,允许实验者测量特异性靶向组蛋白H3赖氨酸27 (H3- K27)的单个组蛋白甲基转移酶的活性或抑制。 该试剂盒可随时使用,并提供进行成功的HMT活性/抑制实验所需的所有必要成分,而不需要放射性或任何特殊设备。
组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)是专为测量组蛋白H3在赖氨酸27的HMT而设计的。 在使用该试剂盒的试验中,组蛋白底物被稳定地捕获在条带孔上。 HMT酶将一个甲基从Adomet转移到组蛋白H3底物,使底物在赖氨酸9处甲基化。 甲基化组蛋白H3-K27可被高亲和力抗体识别。 甲基化H3-K27的比例或数量与酶活性成正比,可以通过HRP共轭的二级抗体显色系统进行定量。 然后根据HMT转化H3-K27的甲基化量计算HMT活性。
组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法),具有如下优势和特点:
- 非常快速的程序,可以在3小时内完成 。
- 安全、创新的比色分析,无需放射性、提取和色谱。
- H3-K27组蛋白甲基转移酶活性/抑制的特异性测定。
- 微孔板条状格式使分析灵活:手工或高通量分析。
- 极其简单、可靠和一致的分析条件。
产品组分
组分内容 |
型号:A-P-3005-48(48次) |
型号:A-P-3005-96(96次) |
储存温度 |
HE1(10X Wash Buffer) | 11ml | 22ml | 4°C |
HE2(Histone Assay Buffer) | 1.5ml | 3ml | 4°C |
HE3(Adomet)* | 25ul | 50ul | -20°C |
HE4(Biotinylated Substrate,25ug/ml)* | 100ul | 200ul | -20°C |
HE5(HMT Standard,10ug/ml)* | 10 ul |
20 ul |
-20°C |
HE6(Capture Antibody,100ug/ml)* | 25 ul |
50 ul |
4°C |
HE7(Detetion Antibody,200ug/ml)* | 10ul | 20ul | -20°C |
HE8(Developer Solution) | 6ml | 12ml | 4°C |
HE9(Stop Solution)* | 3ml | 6ml | 室温 |
Control Enzyme(300ug/ml)* | 5ul | 10ul | -20°C |
8-Well Assay Strips(With Frame) | 6 | 12 | 4°C |
说明书 |
1份 | 1份 |
室温 |
文件资源
文件下载 | 文件内容 | 资源说明 |
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A-P-3005-组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法) | 操作手册 |
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A-P-3005-组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)相关说明 | 宣传单页 |
注意事项
保存建议 | 厂家推荐常温运输。当您收到产品后,按照说明书建议保存。 |
警告 | 一般仅供科研使用,请勿用于临床与诊断。不要将漂白剂或酸性溶液直接添加到样品制备的废物中。 |
FAQ
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