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组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)
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组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)

组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)可从各种生物样品中快速、经济、高通量进行H3K27单个组蛋白甲基转移酶活性检测的试剂盒。更多视频请关注视频号【艾维缔】。
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组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)是一套方便的工具,允许实验者测量特异性靶向组蛋白H3赖氨酸27 (H3- K27)的单个组蛋白甲基转移酶的活性或抑制。 该试剂盒可随时使用,并提供进行成功的HMT活性/抑制实验所需的所有必要成分,而不需要放射性或任何特殊设备。  

组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)是专为测量组蛋白H3在赖氨酸27的HMT而设计的。 在使用该试剂盒的试验中,组蛋白底物被稳定地捕获在条带孔上。 HMT酶将一个甲基从Adomet转移到组蛋白H3底物,使底物在赖氨酸9处甲基化。 甲基化组蛋白H3-K27可被高亲和力抗体识别。 甲基化H3-K27的比例或数量与酶活性成正比,可以通过HRP共轭的二级抗体显色系统进行定量。 然后根据HMT转化H3-K27的甲基化量计算HMT活性。  

组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法),具有如下优势和特点:

  • 非常快速的程序,可以在3小时内完成 。  
  • 安全、创新的比色分析,无需放射性、提取和色谱。
  • H3-K27组蛋白甲基转移酶活性/抑制的特异性测定。 
  • 微孔板条状格式使分析灵活:手工或高通量分析。
  • 极其简单、可靠和一致的分析条件。

产品组分

组分内容

型号:A-P-3005-48(48次)

型号:A-P-3005-96(96次)

储存温度

HE1(10X Wash Buffer) 11ml 22ml 4°C
HE2(Histone Assay Buffer) 1.5ml 3ml 4°C
HE3(Adomet)* 25ul 50ul -20°C
HE4(Biotinylated Substrate,25ug/ml)* 100ul 200ul -20°C
HE5(HMT Standard,10ug/ml)* 10 ul

20 ul

-20°C
HE6(Capture Antibody,100ug/ml)* 25 ul

50 ul

4°C
HE7(Detetion Antibody,200ug/ml)* 10ul 20ul -20°C
HE8(Developer Solution) 6ml 12ml 4°C
HE9(Stop Solution)* 3ml 6ml 室温
Control Enzyme(300ug/ml)* 5ul 10ul -20°C
8-Well Assay Strips(With Frame) 6 12 4°C

说明书

1份 1份

室温

*注意:使用各溶液之前,将溶液离心至管底

文件资源

文件下载 文件内容 资源说明
A-P-3005-组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法) 操作手册
A-P-3005-组蛋白H3K27甲基转移酶活性/抑制分析试剂盒(比色法)相关说明 宣传单页

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保存建议 厂家推荐常温运输。当您收到产品后,按照说明书建议保存。
警告 一般仅供科研使用,请勿用于临床与诊断。不要将漂白剂或酸性溶液直接添加到样品制备的废物中。

 

FAQ

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Huq S et. al. (June 2020). Repurposing the FDA-Approved Antiviral Drug Ribavirin as Targeted Therapy for Nasopharyngeal Carcinoma. Mol Cancer Ther.

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