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铁离子检测试剂盒
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铁离子检测试剂盒

铁离子检测试剂盒,QuantiChrom™ Iron Assay Kit,用于定量检测铁离子 Fe3+ 和/或 Fe2+,以及评估药物对铁代谢的影响。 更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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血液中的铁含量是各种疾病的可靠诊断指标。血液中铁浓度水平升高与失血、红细胞破坏增加(如出血)或血细胞存活率降低、急性肝炎、某些副贫血症、摄入富含铁的饮食、铁储存缺陷(如恶性贫血)有关。饮食中摄入的铁不足、慢性失血性疾病或正常怀孕期间对铁储存的需求增加,都可能导致血铁水平下降。简单、直接、可自动化的铁浓度测量程序在研究、药物发现和环境监测中有着广泛的应用。BioAssay Systems 铁测定试剂盒可直接测定血清中的总铁,无需任何预处理。改进后的方法使用了一种能与 Fe2+ 形成蓝色复合物的发色剂。样品中的 Fe3+ 会被还原成 Fe2+,从而检测出总铁浓度。在 590nm 波长下测量的颜色强度与样品中的铁浓度成正比。

铁离子检测试剂盒具有如下优势和特性:

灵敏、准确。在 96 孔板检测中,铁的线性检测范围为 27 µg/dL (4.8 µM) 至 1,000 µg/dL (179 µM)。
简单、高通量。只需加入一种工作试剂并孵育 40 分钟。可随时自动进行高通量检测,每天可检测数千个样本。
提高试剂稳定性和多功能性。优化的配方大大提高了试剂和信号的稳定性。比色皿或 96 孔板检测。
对生物样本干扰小。无需预处理。可直接对血清样本进行检测。

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铁离子检测试剂盒 DIFE-250 250次 2-8°C

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保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

 

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Hendricks, MR et al (2021). Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection. Cell Reports, 34(4), 108672. Assay: Iron in human extracelllular vesicles.

Hassan AT, Kwong R (2020). The neurophysiological effects of iron in early life stages of zebrafish. Environmental Pollution, 267, 115625. Assay: Iron in water.

Liao, D et al (2020). Identification of pannexin 2 as a novel marker correlating with ferroptosis and malignant phenotypes of prostate cancer cells. OncoTargets and Therapy, 13, 4411-4421. Assay: Iron in human prostate cancer cell lysates.

Salama SA, Omar HA (2021). Modulating NF-kB, MAPK, and PI3K/AKT signaling by ergothioneine attenuates iron overload-induced hepatocellular injury in rats. Journal of Biochemical and Molecular Toxicology, e22729. Assay: Iron in rat.

Elhassanny, AEM et al (2020). Heme-dependent er stress apoptosis: A mechanism for the selective toxicity of the dihydroartemisinin, nsc735847, in colorectal cancer cells. Frontiers in Oncology, 10. Assay: Iron in human colon cancer cell lysates.

Shan, Y et al (2020). Ubiquitin-like modifier activating enzyme 1 as a novel diagnostic and prognostic indicator that correlates with ferroptosis and the malignant phenotypes of liver cancer cells. Frontiers in Oncology, 10, 592413. Assay: Iron in human liver cancer cell lysates.

Chen, K et al (2019). Transcription analysis of the stress and immune response genes to temperature stress in ostrinia furnacalis. Frontiers in Physiology, 10, 1289. Assay: Iron in ostrinia furnacalis plasma.

Hernandez, EP et al. (2020). Expression analysis of glutathione S-transferases and ferritins during the embryogenesis of the tick Haemaphysalis longicornis. Heliyon, 6(3), e03644. Assay: Iron in haemaphysalis longicornis egg homogenates.

Deng, Q et al (2021). Salmonella effector SpvB aggravates dysregulation of systemic iron metabolism via modulating the hepcidin-ferroportin axis. Gut Microbes, 13(1), 1-18. Assay: Iron in mouse serum and liver.

Tang, LJ et al (2021). Ubiquitin-specific protease 7 promotes ferroptosis via activation of the p53/TfR1 pathway in the rat hearts after ischemia/reperfusion. Free Radical Biology & Medicine, 162, 339-352. Assay: Iron in rat cardiac tissue homogenates.

Sanyear, C et al (2020). Iron homeostasis in a mouse model of thalassemia intermedia is altered between adolescence and adulthood. PeerJ, 8, e8802. Assay: Iron in mouse serum.

Lyu, S et al (2020). Deficiency of Meis1, a transcriptional regulator, in mice and worms: Neurochemical and behavioral characterizations with implications in the restless legs syndrome. Journal of Neurochemistry, 155(5), 522-537. Assay: Iron in mouse serum.

Furihata, T et al (2021). Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Communications Biology, 4(1), 138. Assay: Iron in mouse mitochondria.

Lyu, S et al (2020). Mu opioid receptor knockout mouse: Phenotypes with implications on restless legs syndrome. Journal of Neuroscience Research, 98(8), 1532-1548. Assay: Iron in mouse.

Matte JJ, Audet I (2020) Maternal perinatal transfer of vitamins and trace elements to piglets. Animal, 14(1), 31-38. Assay: Iron in pig serum.

Yang, B et al (2020). Ferrous-iron-activated transcriptional factor adhr regulates redox homeostasis in clostridium beijerinckii. Applied and Environmental Microbiology, 86(7). Assay: Iron in clostridium beijerinckii AdHr protein.

Pease, NA et al (2020). Dek expression in breast cancer cells leads to the alternative activation of tumor associated macrophages. Cancers, 12(7). Assay: Iron in mouse cell lysates.

Oliveira, R et al (2020). The novel ECF56 SigG1-RsfG system modulates morphological differentiation and metal-ion homeostasis in Streptomyces tsukubaensis. Scientific Reports, 10(1), 21728. Assay: Iron in streptomyces tsukubaensis cell culture supernatant.

Santiago Gonzalez, DA et al (2019). Iron metabolism in the peripheral nervous system: The role of dmt1, ferritin, and transferrin receptor in schwann cell maturation and myelination. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience, 39(50), 9940-9953. Assay: Iron in mouse DRG explant cultures and sciatic nerve homogenates.

Dichtl, S., Demetz, E., Haschka, D., Tymoszuk, P., Petzer, V., Nairz, M. & Theurl, I. (2019). Dopamine Is a Siderophore-Like Iron Chelator That Promotes Salmonella enterica Serovar Typhimurium Virulence in Mice. mBio, 10(1), e02624-18. Assay: Iron in mice serum.

Malhotra, H., Kumar, M., Chauhan, A. S., Dhiman, A., Chaudhary, S., Patidar, A. & Raje, M. (2019). Moonlighting Protein Glyceraldehyde-3-Phosphate Dehydrogenase: A Cellular Rapid-Response Molecule for Maintenance of Iron Homeostasis in Hypoxia. Cellular physiology and biochemistry: international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(3), 517-531. 
Assay: Iron in mouse cells.

Cheli, V. T., Gonzalez, D. A. S., Marziali, L. N., Zamora, N. N., Guitart, M. E., Spreuer, V. & Paez, P. M. (2018). The Divalent Metal Transporter 1 (DMT1) is required for iron uptake and normal development of oligodendrocyte progenitor cells. Journal of Neuroscience, 38(43), 9142-9159. Assay: Iron in mice cells.

Samba Mondonga, M., Calve, A., Mallette, F. A., & Santos, M. M. (2018). MyD88 regulates the expression of SMAD4 and the iron regulatory hormone hepcidin in hepatoma cells. Frontiers in cell and developmental biology, 6, 105. Assay: Iron in human kidney cells.

Worley, B. L., Kim, Y. S., Mardini, J., Zaman, R., Leon, K. E., Vallur, P. G. & Phaeton, R. (2018). GPx3 supports ovarian cancer progression by manipulating the extracellular redox environment. Redox Biology pii: S2213-2317(18)30891-7. Assay: Iron in human ovarian cells.

Asshoff, M., Petzer, V., Warr, M. R., Haschka, D., Tymoszuk, P., Demetz, E. & Fowles, P. (2017). Momelotinib inhibits ACVR1/ALK2, decreases hepcidin production, and ameliorates anemia of chronic disease in rodents. Blood, 129(13), 1823-1830. Assay: Iron in rat/mice serum.

Ma, X., Pham, V. T., Mori, H., MacDougald, O. A., Shah, Y. M., & Bodary, P. F. (2017). Iron elevation and adipose tissue remodeling in the epididymal depot of a mouse model of polygenic obesity. PloS one, 12(6), e0179889. Assay: Iron in mice serum.

Marks, E. S., Bonnemaison, M. L., Brusnahan, S. K., Zhang, W., Fan, W., Garrison, J. C., & Boesen, E. I. (2017). Renal iron accumulation occurs in lupus nephritis and iron chelation delays the onset of albuminuria. Scientific reports, 7(1), 12821. Assay: Iron in mice plasma.

Quan, Y. Y., Liu, Y. H., Lin, C. M., Wang, X. P., & Chen, T. S. (2017). Peroxynitrite dominates sodium nitroprusside-induced apoptosis in human hepatocellular carcinoma cells. Oncotarget 8(18): 29833-29845. Assay: Iron in cells.

Shang, Y. M., Wang, G. S., Sliney, D. H., Yang, C. H., & Lee, L. L. (2017). Light-emitting-diode induced retinal damage and its wavelength dependency in vivo. International journal of ophthalmology 10(2): 191-202. Assay: Iron in Sprague Dewley rats retina protein.

Sun, X., Zhao, Y., Jia, J., Xie, J., Cheng, J., Liu, H. & Fu, Y. (2017). Uninterrupted expression of CmSIT1 in a sclerotial parasite Coniothyrium minitans leads to reduced growth and enhanced antifungal ability. Frontiers in microbiology, 8, 2208. Assay: Iron in Coniothyrium minitans cells.

Zhang, C. W., Tai, Y. K., Chai, B. H., Chew, K. C., Ang, E. T., Tsang, F. & Lim, K. L. (2017). Transgenic mice overexpressing the divalent metal transporter 1 exhibit iron accumulation and enhanced parkin expression in the brain. Neuromolecular medicine, 19(2-3), 375-386. Assay: Iron in mouse feces.

Hendricks, M. R., Lashua, L. P., Fischer, D. K., Flitter, B. A., Eichinger, K. M., Durbin, J. E. & Bomberger, J. M. (2016). Respiratory syncytial virus infection enhances Pseudomonas aeruginosa biofilm growth through dysregulation of nutritional immunity. Proceedings of the National Academy of Sciences, 113(6), 1642-1647. Assay: Iron in human cells.

Karoopongse, E., Marcondes, A. M., Yeung, C., Holman, Z., Kowdley, K. V., Campbell, J. S., & Deeg, H. J. (2016). Disruption of iron regulation after radiation and donor cell infusion. Biology of Blood and Marrow Transplantation, 22(7), 1173-1181. Assay: Iron in mouse serum.

Li, Y., Pan, K., Chen, L., Ning, J. L., Li, X., Yang, T. & Tao, G. (2016). Deferoxamine regulates neuroinflammation and iron homeostasis in a mouse model of postoperative cognitive dysfunction. Journal of neuroinflammation, 13(1), 268. Assay: Iron in mice cells.

Noguchi-Sasaki, M., Sasaki, Y., Shimonaka, Y., Mori, K., & Fujimoto-Ouchi, K. (2016). Treatment with anti-IL-6 receptor antibody prevented increase in serum hepcidin levels and improved anemia in mice inoculated with IL-6-producing lung carcinoma cells. BMC Cancer 16: 270. Assay: Iron in mouse serum.

Tamayo, E., Benabdellah, K., & Ferrol, N. (2016). Characterization of three new glutaredoxin genes in the arbuscular mycorrhizal fungus Rhizophagus irregularis: putative role of RiGRX4 and RiGRX5 in iron homeostasis. PloS one, 11(2), e0149606. Assay: Iron in yeast cells.

Theurl, I., Hilgendorf, I., Nairz, M., Tymoszuk, P., Haschka, D., Asshoff, M. & Sopper, S. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945. Assay: Iron in human plasma.

Anderson ER, et al (2011). Intestinal hypoxia-inducible factor-2alpha (HIF-2alpha) is critical for efficient erythropoiesis. J Biol Chem. 286(22):19533-40. Assay: Iron in mice serum.

Hamlin F, Latunde-Dada GO (2011). Iron bioavailibity from a tropical leafy vegetable in anaemic mice. Nutr Metab (Lond). 8:9. Assay: Iron in mice serum.

Ringseis R, et al (2010). Low availability of carnitine precursors as a possible reason for the diminished plasma carnitine concentrations in pregnant women. BMC Pregnancy Childbirth.10:17. Assay: Iron in human plasma.

Zhang Y, et al (2010). Diphthamide biosynthesis requires an organic radical generated by an iron-sulphur enzyme. Nature 465(7300):891-6. Assay: Iron in bacterial cell .

Zhu W, et al (2010). Genetic iron chelation protects against proteasome inhibition-induced dopamine neuron degeneration. Neurobiol Dis. 37(2):307-13. Assay: Iron in human neuron cell.

Chen H, et al (2009). Changes in iron-regulatory proteins in the aged rodent neural retina. Neurobiol Aging. 30(11):1865-76. Assay: Iron in rat serum.

Chen H, et al (2009). Dysfunction of the retinal pigment epithelium with age: increased iron decreases phagocytosis and lysosomal activity. Invest Ophthalmol Vis Sci. 50(4):1895-902. Assay: Iron in rat serum.

Shah, YM et al (2009). Intestinal hypoxia-inducible transcription factors are essential for iron absorption following iron deficiency. Cell Metab. 9(2):152-64. Assay: Iron in mouse erythrocytes.

Sudarshan S, et al (2009). Fumarate hydratase deficiency in renal cancer induces glycolytic addiction and hypoxia-inducible transcription factor 1alpha stabilization by glucose-dependent generation of reactive oxygen species. Mol Cell Biol. 29(15):4080-90. Assay: Iron in human tumor cell.

Yokosho K, et al (2009). OsFRDL1 is a citrate transporter required for efficient translocation of iron in rice. Plant Physiol. 149(1):297-305. Assay: Iron in plant rice.

Bandyopadhyay S, et al (2008). A proposed role for the Azotobacter vinelandii NfuA protein as an intermediate iron-sulfur cluster carrier. J Biol Chem. 283(20):14092-9. Assay: Iron in bacterial cell protein.

Raulfs EC, et al (2008). In vivo iron-sulfur cluster formation. Proc Natl Acad Sci U S A. 105(25):8591-6. Assay: Iron in bacterial cell protein.

Habel ME, Jung D. (2006) c-Myc over-expression in Ramos Burkitt’s lymphoma cell line predisposes to iron homeostasis disruption in vitro. Biochem Biophys Res Commun. 341(4):1309-16. Assay: Iron in human lymphoma cell.

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