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高浓度ddCTP
ddCTP的结构式
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H16N3O12P3 (free acid)
Molecular Weight: 451.16 g/mol (free acid)
Exact Mass: 450.99 g/mol (free acid)
CAS#: 66004-77-1
Purity: ≥ 98 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
SNP: multiplexing by minisequencing[1]
Multiplexed detection of alternatively spliced transcripts[2]
Effect on reverse transcriptase of HIV-1[3]
Molecular dynamic calculations of DNA-polymerase beta-ddCTP complex[4]
Crystal structure with DNA- polymerase-I[5]
产品组分
内容 | 型号 | 规格 |
储存温度 |
高浓度ddCTP | NU272-01 | 10ul(1uM) | -20°C |
高浓度ddCTP | NU272-02 | 50ul(5uM) | -20°C |
操作手册 |
1 |
常温 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
Selected References:
[1] Gronlund et al. (2011) Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap. Preparative Biochemistry and Biotechnology 41:166.
[2] Milani et al. (2006) Detection of alternatively spliced transcripts in leukemia cell lines by minisequencing on microarrays. Clinica Chemistry (Washington) 52:202.
[3] Anderson (2001) The molecular basis of inhibition and toxicity of modified cytosine analogues targetting HIV-1 reverse transcriptase. Antiviral Chemistry and Chemotherapy 12:13.
[4] Rittenhouse et al. (2003) Characterization of the active site of DNA polymerase beta by molecular dynamics and quantum chemical calculation. Proteins: Structure, Function and Genetics 53:667.
[5] Li et al. (2001) Crystal structures of ddATP-, ddTTP-, ddCTP-, and ddGTP-trapped ternary complex of Klentaq1: insights into nucleotide incorporation selectivity. Protein Science 10:1225.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
Microtubule-dependent apical polarization of basement membrane matrix mRNAs in mouse epithelial cells.
Shaohe Wang, Kazue Matsumoto, ..., Kenneth M Yamada
Cells & development | 2023 Dec 14 | PubMed ID: 38103869
Reverse transcriptases prime DNA synthesis
Matej Zabrady, Katerina Zabrady, ..., Aidan J Doherty
Nucleic Acids Research | 2023 Aug 11 | PubMed ID: 37279911
Functional and molecular dissection of HCMV long non-coding RNAs
Sungwon Lee, Hyewon Kim, ..., Kwangseog Ahn
Scientific Reports | 2022 Nov 11 | PubMed ID: 36369338
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