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描述: Rabbit polyclonal antibody to STAT3 应用: WB IHC IF/ICC 文献验证: WB, IHC, IF/ICC 反应: Human, Mouse, Rat 预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken 分子量: 86kDa; 88kD(Calculated). 蛋白号: P40763 RRID: AB_2835144 来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(91%), Sheep(82%), Rabbit(91%), Chicken(91%)
克隆:
Polyclonal
特异性:
STAT3 Antibody detects endogenous levels of total STAT3.
RRID:
AB_2835144
引用格式: Affinity Biosciences Cat# AF6294, RRID:AB_2835144.偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
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产品组分
内容 型号
规格 储存温度
STAT3抗体 AF6294-01 50ul -20°C STAT3抗体 AF6294-02 100ul -20°C STAT3抗体 AF6294-03 200ul -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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1). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]
Application: WB Species: Mice Sample:
Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)
2). CYSLTR1 antagonist inhibits Th17 cell differentiation by regulating the NF-κB signaling for the treatment of psoriasis. International journal of biological sciences, 2024 (PubMed: 38617532) [IF=8.2]
3). Tofacitinib Promotes Functional Recovery after Spinal Cord Injury by Regulating Microglial Polarization via JAK/STAT Signaling Pathway. International Journal of Biological Sciences, 2023 (PubMed: 37781508) [IF=8.2]
4). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024 (PubMed: 39613048) [IF=7.1]
5). Apigenin ameliorates imiquimod-induced psoriasis in C57BL/6J mice by inactivating STAT3 and NF-κB. Food Science and Human Wellness, 2024 [IF=7.0]
Application: WB Species: Mouse Sample: skin tissues
Figure 2 Gla ameliorated liver inflammation induced by ethanol via activating the NF-κB pathway in vivo. (A–D) Protein levels of p-NF-κB, NF-κB, IL-6, TNF-α, NLRP3, pro-caspase1, cleaved-caspase1, ASC, pro-IL-1β, and IL-1β were determined using Western blot method (n = 6). (E–G) mRNA levels of Nlrp3, Caspase1, and Asc were determined by qPCR (n = 5). (H–J) Serum levels of IL-1β, IL-6, and TNF-α (n = 5). ns, not significant; * p < 0.05; ** p < 0.01 vs. “Con” group; # p < 0.05, ## p < 0.01 vs. “EtOH” group.
6). Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats. ACTA PHARMACOLOGICA SINICA, 2020 (PubMed: 31848475) [IF=6.9]
Application: WB Species: Rat Sample: rat aortic endothelial cells (RAECs)
Fig. 1 ASM downregulation improved PA-induced leptin resistance in RAECs. Western blot gels and summarized data showing the protein expression of Ob-Rb (a) and SOCS3 (b) in RAECs treated with PA (0.1, 0.2, and 0.3 mM) with or without 100 nM leptin. RAECs were incubated with 0.3 mM PA for 24 h and were then transfected with ASM siRNA for 24 h. Then, 100 nM leptin was added for 15 min prior to the collection of the cells. Then, Western blotting and an AmpliteTM Fluorimetric Kit were used to test the protein expression of ASM (c) and ASM activity (d), respectively, in RAECs. Representative fluorescence images (e, scale bar = 20 μm) and summarized data showing the mean fluorescent intensity (MFI) of a FITC-labeled anti-ceramide antibody (f). The photographs were taken at ×400 magnification. The protein expression of ObRb (g) and SOCS3 (h) and the ratio of p-STAT3/STAT3 (i) was determined using Western blotting. The data are the means ± SEMs from three experiments. *P < 0.05 vs. scramble control (Scr Ctrl); #P < 0.05 vs. the PA alone-treated group
7). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]
8). Formononetin protects against inflammation associated with cerebral ischemia-reperfusion injury in rats by targeting the JAK2/STAT3 signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 35339827) [IF=6.9]
9). PTPN2 dephosphorylates STAT3 to ameliorate anesthesia-induced cognitive decline in aged rats by altering the microglial phenotype and inhibiting inflammation. Biochimica et biophysica acta. Molecular basis of disease, 2025 (PubMed: 39481492) [IF=6.2]
10). The synthesis and bioactivity of pyrrolo[2,3-d]pyrimidine derivatives as tyrosine kinase inhibitors for NSCLC cells with EGFR mutations. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34315040) [IF=6.0]
Application: WB Species: Human Sample: HBE, A549, NCIeH1975, HCC827 cell
Fig. 4. Inhibition of the phosphorylation of the EGFR signaling pathway by compound 12h (A), 12i (B) and avitinib (C) in HBE, A549, NCIeH1975, HCC827 cell lines.
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