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dPTP

dPTP,6H,8H-3,4-Dihydro-pyrimido(4,5-c)(1,2)oxazin-7-one-8-β-D-2'-deoxy-ribofuranoside-5'-triphosphate, Sodium salt,6H,8H-3,4-二氢-嘧啶并(4,5-c)(1,2)恶嗪-7-酮-8-β-D-2'-脱氧-呋喃核糖苷-5'-三磷酸酯钠盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。


关键词

dPTP


所属分类


目录号

NU1119

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  • For general laboratory use.

    Please centrifuge briefly before opening (volume ≤2 ml).

    Shipping: shipped on gel packs

    Storage Conditions: store at -20 °C
    Short term exposure (up to 1 week cumulative) to ambient temperature possible.

    Shelf Life: 12 months after date of delivery

    Molecular Formula: C11H18N3O14P3 (free acid)

    Molecular Weight: 509.19 g/mol (free acid)

    Exact Mass: 509.00 g/mol (free acid)

    CAS#: 173964-83-5

    Purity: ≥ 95 % (HPLC)

    Form: solution in water

    Color: colorless to slightly yellow

    Concentration: 10 mM - 11 mM

    pH: 7.5 ±0.5

    Spectroscopic Properties: λmax 294 nm, ε 6.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

    Applications:
    Mutagenesis via reverse transcription[1]
    Interaction with DNA polymerases alpha and I[2]
    Incorporation rate by terminal transferase[3]
    Generation of a library of mutants[4]

  • 产品组分

    内容

    型号

    规格

    储存温度

    dPTP

    NU1119-01

    30ul(10mM)

    室温

    dPTP

    NU1119-02

    150ul(10mM)

    室温

    操作手册

    1 1

    常温

  • 文件资源

    文件下载

    文件内容

    资源说明

    NU1119-dPTP

    中文手册

    NU1119-dPTP相关单页

    宣传单页

     

  • 营销中心1

  • 注意事项

    保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

     

  • FAQ

  • Selected References:
    [1] Petrie et al. (2010) Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs. Nucleic Acids Res. 38 (22):8095.
    [2] Patro et al. (2009) Interaction of human DNA polymerases alpha and DNA polymerase I from Bacillus stearothermophilus with hypoxanthine and 8-oxoguanine nucleotides. Biochemistry48:8271.
    [3] Wong et al. (2008) Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complement the bias of error-prone PCR. Biotechnology J.3:74.
    [4] Cain et al. (2001) Selection of novel ligands from a whole -molecular randomly mutated C5a library. Protein Engineering14:189.
    Huber et al. (2010) Amino acid residues important for folding of thioredoxin are revealed only by study of the physiologically relevant reduced form of the protein. Biochemistry 49 (41):8922.
    Cummins et al. (2001) Synthesis and study of the fluorescein conjugate of the nucleotide dPTP. Nucleos. Nucleot. Nucl. 20 (4-7):1049.
    Zaccolo et al. (1999) The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase. Journal of Molecular Biology 285 (2):775.
    Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589.
    Lethal mutagenesis: Loeb, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1492.

     

    ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples

    Zahm Adam M., Cranney Caleb W., ..., English Justin G.

    bioRxiv  |  2025 Apr 06

    Multiplexed no-wash cellular imaging using BenzoTag, an evolved self-labeling protein

    Bryan J. Lampkin, Benjamin J. Goldberg, Joshua A. Kritzer

    Chemical Science  |  2024 Oct 09  |  39430930

    Protocol for engineering binding domains to recognize ligand-bound receptors by using yeast surface display

    Markus Dobersberger, Delia Sumesgutner, ..., Michael W. Traxlmayr

    STAR Protocols  |  2024 Sep 24  |  39321026

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