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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C17H23N2O14P3 (free acid)
Molecular Weight: 572.29 g/mol (free acid)
Exact Mass: 572.04 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: ε292 nm 11.0 L mmol-1 cm-1 (in Tris-HCl pH 7.5)
Applications:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice- to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
- to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Presolski et al.[3] and Hong et al.[4] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
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产品组分
内容
型号
规格 储存温度
C8-炔基-dUTP
CLKT05-01 10uL(100mM)
-20°C C8-炔基-dUTP
CLKT05-02 50uL(100mM)
-20°C C8-炔基-dUTP
CLKT05-03 100uL(100mM)
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.
Alex M Yoshikawa, Alexandra Rangel, ..., H Tom Soh
Nature Communications | 2021 Dec 29 | 34876561
Supplementary Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification
Ysobel R Baker, Liyiwen Yuan, ..., Tom Brown
Nucleic Acids Research | 2021 Sep 20 | 34403467
Patent Glycosylated oligonucleotides that mimic the HIV-1 epitope of neutralizing monoclonal antibody 2G12
Isaac J. Krauss, Joel Sebastian Temme
us10780150 | 2020 Sep 22
Patent High temperature selection of nucleotide-supported carbohydrate vaccines and resulting glycosylated oligonucleotides
Isaac J. Krauss
us10378017 | 2019 Aug 13
Supplementary Nucleotidyl transferase assisted DNA labeling with different click chemistries
Marie-Luise Winz, Eva Christina Linder, ..., Andres Jäschke
Nucleic Acids Research | 2015 Sep 30 | 26013812
Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA
Suzana Kovacic, Laleh Samii, ..., Nancy R. Forde
Biomacromolecules | 2014 Oct 07
Design and Construction of a One-Dimensional DNA Track for an Artificial Molecular Motor
Suzana Kovacic, Laleh Samii, ..., Gerhard A. Blab
Journal of Nanomaterials | 2012 Jan 01
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