高保真核心试剂盒

高保真核心试剂盒，High Fidelity Core Kit，用于高精度的耐热DNA聚合酶、dNTPs及反应缓冲液套装。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

关键词

高保真核心试剂盒

所属分类

分子生物学

目录号

PCR234

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For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
High Fidelity Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. The premium quality polymerase, ultrapure dNTPs and the optimized complete reaction buffer ensure superior amplification results.
High Fidelity Pol is based on a blend of Taq DNA polymerase and a proofreading enzyme specially designed for highly accurate and efficient amplification. It shows excellent results with extremely long (up to 30 kb), GC-rich or other difficult templates.
The enzyme blend includes a highly processive 5'→3' DNA polymerase and possesses a 5'→3' polymerization-dependent exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase.
The enzyme is highly purified and free of bacterial DNA.

Fidelity of the enzyme:
High Fidelity Pol is characterized by a 4-fold higher fidelity compared to Taq polymerase.
ER_{High Fidelity Pol} = 3.4 x 10^-6
The error rate (ER) of a PCR reaction is calculated using the equation ER = MF/(bp x d), where MF is the mutation frequency, bp is the number of base pairs of the fragment and d is the number of doublings
(2^d = amount of product / amount of template).

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74 °C.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 2.5 units/μl

Content:
High Fidelity Pol (red cap)
2.5 units/μl High Fidelity Polymerase in storage buffer

dNTP Mix (white cap)
10 mM each dNTP (dATP, dCTP, dGTP, dTTP)

High Fidelity Buffer (green cap)
10x conc

Recommended 50 μl PCR assay:

5 μl	10x High Fidelity Buffer	green cap
1 μl	dNTP Mix	white cap
0.2 - 0.5 μM	each Primer	-
1 - 100 ng	template DNA	-
0.5 μl (1.25 units)	High Fidelity Pol	red cap
Fill up to 50 μl	PCR-grade water	-

Please note that it is essential to add the polymerase as last component.

Recommended cycling conditions:

initial denaturation	94 °C	2 min	1x
denaturation	94 °C	20 sec	20-30x
annealing¹⁾	50 - 68 °C	30 sec	20-30x
elongation²⁾	68 °C	1 min/kb	20-30x
final elongation	68 °C	1 min/kb	1x

¹⁾The annealing temperature depends on the melting temperature of the primers used.
²⁾The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

For optimal specificity and amplification an individual optimization of the recommended parameters may be necessary for each new template DNA and/or primer pair.

产品组分

内容	型号	规格	储存温度
高保真核心试剂盒	PCR234-01	200Units	-20°C
高保真核心试剂盒	PCR234-02	1000Units	-20°C
操作手册	1	1	常温

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保存建议厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。
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