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For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice- to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C12H20N3O14P3 (free acid)
Molecular Weight: 523.22 g/mol (free acid)
Exact Mass: 523.02 g/mol (free acid)
CAS#: 116840-18-7
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by- PCR with Taq polymerase[1], in-house data
- Nick Translation with DNAse I/ DNA Polymerase I[2]
- Primer Extension with Klenow exo- [3]
- 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4]
- Reverse Transcription with MMLV Reverse Transcriptase[2]
Selected References:
[1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
[2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
[3] Cherkasov et al. (2010) New Nucleotide Analogues with Enhanced Signal Properties. Bioconjugate Chem. 21 (1):122.
[4] Unciti-Broceta et al. (2003) The use of solid supports to generate nucleic acid carriers.Accounts of Chemical Research 45:1140. -
产品组分
内容 型号
规格 储存温度
烯丙胺-dUTP溶液
NU80S-01 20uL(100mM)
-20°C 烯丙胺-dUTP溶液
NU803-02 50uL(100mM)
-20°C 烯丙胺-dUTP溶液
NU803-03 100uL(100mM)
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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