- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For research use only.
Description:
Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice- to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C12H20N3O14P3 (free acid)
Molecular Weight: 523.22 g/mol (free acid)
Exact Mass: 523.02 g/mol (free acid)
CAS#: 116840-18-7
Purity: ≥ 95 % (HPLC)
Form: solid
Color: white to off-white
Spectroscopic Properties: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by- PCR with Taq polymerase[1], in-house data
- Nick Translation with DNAse I/ DNA Polymerase I[2]
- Primer Extension with Klenow exo- [3]
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4]
- Reverse Transcription with MMLV Reverse Transcriptase[2]
Selected References:
[1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
[2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
[3] Cherkasov et al. (2010) New Nucleotide Analogues with Enhanced Signal Properties. Bioconjugate Chem. 21 (1):122.
[4] Unciti-Broceta et al. (2003) The use of solid supports to generate nucleic acid carriers.Accounts of Chemical Research 45:1140. -
产品组分
内容 型号
规格 储存温度
烯丙胺-dUTP粉末
NU8031-01 1mg
-20°C 烯丙胺-dUTP粉末
NU8031-02 5mg
-20°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Patent PCR-free library preparation using double-stranded splint adaptors and methods of use
Junhua Zhao, Xiaodong Qi, Shawn Levy
us12606819 | 2026 Apr 21
Patent Method and components for detecting nucleic acid chains
Dmitry Cherkasov, Claus Becker, ..., Petra Van Husen
us09994894 | 2018 Jun 12
Patent Conjugates of nucleotides and method for the application thereof
Claus Becker, Dmitry Cherkasov
us09315860 | 2016 Apr 19
Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production.
Susanne Roth, Andrea Rottach, ..., Jürgen Ruland
Nature immunology | 2014 Jul 28 | 24777530
猜你喜欢
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
