生物素-11-dCTP
生物素-11-dCTP，Biotin-11-dCTP，Biotin-X-5-Propargylamino-dCTP，生物素-X-5-炔丙胺基-dCTP，γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt，γ-[N-(生物素-6-氨基己酰基)]-5-丙炔氨基-2'-脱氧胞苷-5'-三磷酸三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
关键词
生物素-11-dCTP
所属分类
目录号
NU809BIO11
- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C28H44N7O16P3S (free acid)
Molecular Weight: 859.67 g/mol (free acid)
Exact Mass: 859.18 g/mol (free acid)
CAS#: 136632-30-9
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
FISH[1, 2]
SNP-analysis[1]
Nick-translation[2]
TUNEL[3]Selected References:
[1] Ye et al. (2001) Fluorescent microsphere-based readout technology for multiplexed human single nucleotide polymorphism analysis and bacterial identification. Human Mutation 17:305.
[2] Backx et al. (2008) Direct fluorescent labelling of clones by DOP PCR. Molecular Cytogenetics 1:3.
[3] Jones et al. (2003) Herpes simplex virus type 2 induces rapid cell death and functional impairment of murine dendritic cells in vitro. J. Virology 77:11139.
Bishop et al. (2008) APOBEC3G Inhibits Elongation of HIV-1 Reverse Transcripts. PLoS Pathogens 4:e1000231. -
产品组分
组分内容
型号
规格
储存温度
生物素-11-dCTP
NU809BIO11-01 200ul（1mM） 2-8°C 生物素-11-dCTP
NU809BIO11-02 1ml（1mM） 2-8°C 操作手册 1 1 常温 -
文件资源
文件下载
文件内容
资源说明
NU809BIO11-生物素-11-dCTP
操作手册 NU809BIO11-生物素-11-dCTP相关说明
宣传单页 -
营销中心
-
注意事项
保存建议 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 -
FAQ
-
Single-cell multiomic QTL mapping reveals state-dependent genetic regulation and associated gene during cellular senescence
Yi Xianfu, Wang Xueqi, ..., Li Mulin Jun
bioRxiv | 2026 Sep 21
Maintenance of nucleosome organization through replication and transcription counteracts aberrant coalescence of active chromatin.
R Ciaran MacKenzie Frater, Rikke Rejnholdt Jensen, ..., Anja Groth
Molecular cell | 2026 Aug 17 | 42607685
Chromatin topology and distal elements underlie divergent cell-type-specific regulation of 9p21 locus cell cycle genes
Elena Torlai Triglia, Tyler E. Miller, ..., Elizabeth Gaskell
Cell Genomics | 2026 Aug 12 | 42520801
猜你喜欢
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
