生物素-11-dATP
生物素-11-dATP，Biotin-11-dCTP，Biotin-X-5-Propargylamino-dCTP，生物素-X-5-炔丙胺基-dCTP，γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt，γ-[N-(生物素-6-氨基己酰基)]-5-丙炔氨基-2'-脱氧胞苷-5'-三磷酸三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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生物素-11-dATP
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NU1175BIOX
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For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).Description:
Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.Recommended Biotin-16-dATP/dATP ratio for PCR: 50% Biotin-16-dATP/ 50% dATP
Please note: The optimal final concentration of Biotin-11-dATP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Biotin-11-dATP/dATP ratio is recommended.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C30H45N8O15P3S (free acid)
Molecular Weight: 882.71 g/mol (free acid)
Exact Mass: 882.19 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [2]
- Primer Extension with Klenow exo- [3,4]
- Reverse Transcription with MMLV Reverse Transcriptase [5]Selected References:
[1] Zammatteo et al. (2005) Unambiguous identification of the expressed MAGE-A genes on a DNA microarray. Clin. Chem. 51 (12):2420.
[2] Dauwerse et al. (1999) Two-colour FISH detection of the inv (16) in interphase nuclei of patients with acute myeloid leukaemia. Br. J. Haematol. 106 (1):111.
[3] Vorwerk et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N. Biotechnol. 25 (2):142.
[4] Beier et al. (2008) Microfluidic primer extension assay. Methods Mol Biol 822:143.
[5] Vankoningsloo et al. (2008) Gene expression silencing with 'specific' small interfering RNA goes beyond specificity - a study of key parameters to take into account in the onset of small interfering RNA off-target effects. FEBS J. 275 (11):2738. -
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生物素-11-dCTP
NU1175BIOX-01 10μL（1mM） 2-8°C 生物素-11-dCTP
NU1175BIOX-02 50μL（1mM） 2-8°C 操作手册 1 1 常温 -
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NU1175BIOX-生物素-11-dATP
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FAQ
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Stage-specific epigenetic priming amplifies gene activation during lineage commitment.
Arion Foertsch, Naama Hirsch, ..., Asifa Akhtar
Science Advances | 2026 Mar 27 | 41894493
STAG2 mutations reshape the cohesin-structured spatial chromatin architecture to drive gene regulation in acute myeloid leukemia.
Alexander Fischer, Benjamín Hernández-Rodríguez, ..., Claudia Gebhard
Cell Reports | 2024 Jul 25 | 39084219
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