亮红

Name: 亮红
Brand: 艾德科技（北京）有限公司 专注于生物领域新产品新技术开发与应用

亮红，JBS Bright Red，Eosin Scarlet，曙红猩红。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

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亮红

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IVDEpi

目录号

CO304

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初次结晶的晶体往往需要经过细致的后续优化，才能成为可用于衍射分析的优质晶体。我们提供一系列针对性产品，用于优化结晶条件、提升晶体质量、助力膜蛋白结晶、观测晶体形态，并辅助解决相位解析相关策略问题。

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用于调控成核、晶体生长、溶剂含量的工具以及晶种策略，以提升重现性和衍射性能。

提供支持高难度膜蛋白靶点结晶的产品，包括脂质立方相产品以及专用结晶工作流程。

晶体染料有助于区分蛋白质晶体与盐晶体，并为筛选和优化过程中的快速目视检查提供支持。

模型蛋白为结晶训练、方法开发、测定验证和质量控制提供了可靠的参考体系。

用于实验性定相策略的试剂和工具，用于在分子置换法不足以支持结构解析时提供支持。

For research use only.

Description:
Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals.

Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals ^[1].

Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS Bright Red, are able to permeate the solvent channels of a protein, thus coloring the protein red. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 12 months

Molecular Formula: C₂₀H₆Br₂N₂Na₂O₉

Molecular Weight: 624.08 g/mol

CAS#: 548-24-3

EC number: 208-943-1

Concentration: 1 mM

Applications:
JBS Bright Red is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.

Usage:
Simply add 0.5-1 μl of JBS Bright Red to the crystallization drop containing the crystals of interest.

Coloring Time:
JBS Bright Red colors protein crystals after a few minutes. Even if the color of the solution is only faintly red under the microscope, proteins will be stained within 5-15 min.
Very occasionally, it has been reported that protein crystals did not absorb crystal dyes ^[2].

Selected References:
[1] Wilkosz et al. (1995) Preliminary characterization of EcoRI-DNA co-crystals: incomplete factorial design of oligonucleotide sequences. Acta Cryst. D 51:938.
[2] Eckert et al. (2003) Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Cryst. D 59:139.
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