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CRISPR/Cas9多克隆抗体
Background
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA, has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.
Concentration
1 mg/ml
Description
Rabbit polyclonal antibody to CRISPR/Cas9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1, with further optimization for IP and ChIP due to recognition of multiple epitopes. This antibody can (a) detect CRISPR/Cas9 expression in target cells by WB, IF, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected; or (b) detect genome-wide off- and on-target effects of CRISPR/Cas9 on specific gene sites by Cas9 ChIP-seq in CRISPR genome editing.
Specificity
Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)
Reactivity:
Species Independent
Isotype
IgG
Formulation
Liquid containing 0.05% Sodium Azide, 1X TBS (pH7.4), 0.5% BSA, 40% Glycerol
Storage
Store at 4°C for 3 weeks. For long term storage, aliquot and then store at −20°C or −80°C. Avoid multiple freeze/thaw cycles.
Purity
Protein A purified
Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap
Alternative Names
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody
Application
WB: (1:2000 - 1:5000), ELISA: (1:5000-1:10000), ChIP or IP: (2 µg/106 cells), IF: (1:200-1:500), ICC: (1:200-1:500)
产品组分
内容 |
型号 |
规格 |
储存温度 |
CRISPR/Cas9多克隆抗体 | A-1111-01 | 10ug | -20°C |
CRISPR/Cas9多克隆抗体 | A-1111-02 | 50ug | -20°C |
操作手册 |
1 | 1 |
常温 |
文件资源
文件下载 | 文件内容 | 资源说明 |
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A-1111-CRISPR/Cas9多克隆抗体 | 操作手册 |
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A-1111-CRISPR/Cas9多克隆抗体相关单页 | 宣传单页 |
注意事项
保存建议 | 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 |
FAQ
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