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CRISPR/Cas9多克隆抗体
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CRISPR/Cas9多克隆抗体

CRISPR/Cas9多克隆抗体,CRISPR/Cas9 Polyclonal Antibody,非常适合开展WB,ELISA,ChIP,IP,IF和ICC实验类型。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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Background
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA, has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.

Concentration 
1 mg/ml

Description 
Rabbit polyclonal antibody to CRISPR/Cas9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1, with further optimization for IP and ChIP due to recognition of multiple epitopes. This antibody can (a) detect CRISPR/Cas9 expression in target cells by WB, IF, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected; or (b) detect genome-wide off- and on-target effects of CRISPR/Cas9 on specific gene sites by Cas9 ChIP-seq in CRISPR genome editing.

Specificity 
Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)

Reactivity:
Species Independent

Isotype 
IgG

Formulation 
Liquid containing 0.05% Sodium Azide, 1X TBS (pH7.4), 0.5% BSA, 40% Glycerol

Storage  
Store at 4°C for 3 weeks. For long term storage, aliquot and then store at −20°C or −80°C. Avoid multiple freeze/thaw cycles.

Purity 
Protein A purified

Handling Recommendations 
For maximum recovery of the products, centrifuge the vial prior to opening the cap

Alternative Names
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody

Application
WB: (1:2000 - 1:5000), ELISA: (1:5000-1:10000), ChIP or IP: (2 µg/106 cells), IF: (1:200-1:500), ICC: (1:200-1:500) 

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储存温度

CRISPR/Cas9多克隆抗体 A-1111-01 10ug -20°C
CRISPR/Cas9多克隆抗体 A-1111-02 50ug -20°C

操作手册

1 1

常温

文件资源

文件下载 文件内容 资源说明
A-1111-CRISPR/Cas9多克隆抗体 操作手册
A-1111-CRISPR/Cas9多克隆抗体相关单页 宣传单页

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注意事项

保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

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