抗LC3多克隆抗体

抗LC3多克隆抗体，Anti-LC3 pAb，非常适合开展WB，IH，IC，IP，IH和FCM等实验类型。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

关键词

抗LC3多克隆抗体

所属分类

精品一抗

目录号

PM036

获取报价

Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB 1:1,000 IP 2 µL/300 µL of cell extract from 1x10⁷ cells FCM 1:200 IC 1:500-1:1,000 IH 1:1,000-1:2,000 Other(Immuno-EM) reported. （PMID: 28063257）
Immunogen (Antigen)	recombinant Human LC3 (MAP1LC3B:1-120 a.a.)
Reactivity [Gene ID]	Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100769810], Zebrafish*[406466](PMID: 28924380/29323117)
Storage buffer	"PBS/50% glycerol, pH 7.2"
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	"MAP1LC3B, LC3B, ATG8F, MAP1LC3B-a, MAP1"
Background	"Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3."

产品组分

内容	型号	规格	储存温度
抗LC3多克隆抗体	PM036	100ul	-20°C
操作手册	1	1	常温室温

文件资源

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注意事项

保存建议厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。
FAQ
Western Blotting
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49. Sun Y et al. Ischemic Postconditioning Alleviates Cerebral Ischemia-Reperfusion Injury Through Activating Autophagy During Early Reperfusion in Rats. Neurochem Res. 43, 1826-1840 (2018)(PMID:30046966)
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Immunoprecipitation
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Immunocytochemistry
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6. Seillier M et al. TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death. Cell Death Differ. 19, 1525-35 (2012)(PMID:22421968)
7. Kaminskyy VO et al. Suppression of basal autophagy reduces lung cancer cell proliferation and enhances caspase-dependent and -independent apoptosis by stimulating ROS formation. Autophagy 8, 1032-44 (2012)(PMID:22562073)
8. Botbol Y et al. Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T-cell activation. Autophagy 11, 1864-77 (2015)(PMID:26391567)
9. Botbol Y, Macian F. Assays for Monitoring Macroautophagy Activity in T cells. Methods Mol Biol. 1343, 143-53 (2015)(PMID:26420715)
10. Cherepkova MY et al. Leukemia inhibitory factor (LIF) withdrawal activates mTOR signaling pathway in mouse embryonic stem cells through the MEK/ERK/TSC2 pathway. Cell Death Dis. 7, e2050 (2016)(PMID:26775702)
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17. Lu SL et al. Endothelial cells are intrinsically defective in xenophagy of Streptococcus pyogenes. PLoS Pathog. 13, e1006444 (2017)(PMID:28683091)
18. Ono A et al. Feedback activation of AMPK-mediated autophagy acceleration is a key resistance mechanism against SCD1 inhibitor-induced cell growth inhibition. PLoS One. 12, e0181243 (2017)(PMID:28704514)
19. Chen YD et al. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells. Sci Rep. 7, 5676 (2017)(PMID:28720835)
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21. Kjos I et al. Rab7b modulates autophagic flux by interacting with Atg4B. EMBO Rep. 18, 1727-1739 (2017)(PMID:28835545)
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24. Lee PP et al. Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells. Nat Commun. 8, 1576 (2017)(PMID:29146903)
25. Yanagisawa H et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci Rep. 7, 15944 (2017)(PMID:29162837)
26. Hsu CL et al. MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Nat Commun. 9, 942 (2018)(PMID:29507340)
27. Sun Y et al. 3′-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Theranostics 8, 2044-2060 (2018)(PMID:29556372)
28. El-Darawish Y et al. Frontline Science: IL-18 primes murine NK cells for proliferation by promoting protein synthesis, survival, and autophagy. J Leukoc Biol. 104, 253-264 (2018)(PMID:29603367)
29. Raulien N et al. Fatty Acid Oxidation Compensates for Lipopolysaccharide-Induced Warburg Effect in Glucose-Deprived Monocytes. Front Immunol. 8, 609 (2017)(PMID:28611773)
30. Härtlova A et al. LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages. EMBO J. 37, e98694 (2018)(PMID:29789389)
31. Phadwal K et al. Spermine increases acetylation of tubulins and facilitates autophagic degradation of prion aggregates. Sci Rep. 8, 10004 (2018)(PMID:29968775)
32. Skah S et al. cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53. Oncotarget 9, 30434–30449 (2018)(PMID:30100998)
33. Hagio-Izaki K et al. Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus. BMC Cell Biol. 19, 18 (2018)(PMID:30165815)
34. Jiao YN et al. Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells. Cancer Cell Int. 18, 149 (2018)(PMID:30275772)
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36. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)
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38. Schulthess J et al. The Short Chain Fatty Acid Butyrate Imprints an Antimicrobial Program in Macrophages. Immunity. 50, 432-445.e7 (2019)(PMID:30683619)
39. Ma B et al. The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression. Nat Commun. 10, 1034 (2019)(PMID:30833558)
40. Ding X et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 15, 1774-1786 (2019)(PMID:30957628)
41. Nakashima A et al. Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts. Sci Rep. 9, 11466 (2019)(PMID:31391477)
42. Nakamura T et al. Soluble Lytic Transglycosylase SLT of Francisella Novicida Is Involved in Intracellular Growth and Immune Suppression. PLoS One. 14, e0226778 (2019) (PMID:31877174)
43. Omi J et al. The Inducible Amphisome Isolates Viral Hemagglutinin and Defends Against Influenza A Virus Infection. Nat Commun. 11, 162 (2020)(PMID:31919357)
44. Dong S et al. Monitoring Spatiotemporal Changes in Chaperone-Mediated Autophagy in Vivo. Nat Commun. 11, 645 (2020)(PMID:32005807)
45. Siedlecka-Kroplewska K et al. The wine polyphenol resveratrol modulates autophagy and induces apoptosis in MOLT-4 and HL-60 human leukemia cells. J Physiol Pharmacol. 70 (2020)(PMID:32084644)
46. Nozaki M et al. SQSTM1 L341V variant that is linked to sporadic ALS exhibits impaired association with MAP1LC3 in cultured cells. eNeurologicalSci. 22, 100301 (2020)(PMID:33319079)
Immunohistochemistry
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2. Girault V et al. Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvessels. Cell Death Dis. 8, e2610 (2017)(PMID:28182007)
3. Qi M et al. ERK inhibition represses gefitinib resistance in non-small cell lung cancer cells. Oncotarget. 9, 12020-12034 (2018)(PMID:29552290)
4. Ko PY et al. Schwann-Cell Autophagy, Functional Recovery, and Scar Reduction After Peripheral Nerve Repair. J Mol Neurosci. 64, 601-610 (2018)(PMID:29644600)
5. Alvarez-Garcia O et al. FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell. 17, e12800 (2018)(PMID:29963746)
6. Horibe A et al. Ethanol-Induced Autophagy in Sertoli Cells Is Specifically Marked at Androgen-Dependent Stages of the Spermatogenic Cycle: Potential Mechanisms and Implications. Int J Mol Sci. 20, pii: E184 (2019)(PMID:30621351)
7. Lu SZ et al. Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation. Transl Neurodegener. 8, 3 (2019)(PMID:30675347)
8. Hsu YH et al. Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo. Free Radic Biol Med. 135, 235-244 (2019)(PMID:30878646)
9. Eid N et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy. Cells. 8, pii: E283 (2019)(PMID:30934625)
10. Boukhalfa A et al. PI3KC2α-dependent and VPS34-independent Generation of PI3P Controls Primary Cilium-Mediated Autophagy in Response to Shear Stress. Nat Commun. 11, 294 (2020) (PMID:31941925)
11. Ogawa-Akiyama A et al. Podocyte Autophagy Is Associated With Foot Process Effacement and Proteinuria in Patients With Minimal Change Nephrotic Syndrome. PLoS One. 15, e0228337 (2020)(PMID:31978139)
12. Kurashige T et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J Endocr Soc. 4, bvaa054 (2020)(PMID:32671315)
Immunofluorescence
1. Nakajima S et al. Fat-specific protein 27α inhibits autophagy-dependent lipid droplet breakdown in white adipocytes. J Diabetes Investig. in press.(PMID:30927519)
2. Scotto Rosato A et al. TRPML1 Links Lysosomal Calcium to Autophagosome Biogenesis Through the Activation of the CaMKKβ/VPS34 Pathway. Nat Commun. 10, 5630 (2019)(PMID:31822666)
3. Luciani A et al. Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nat Commun. 11, 970 (2020)(PMID:32080200)
4. Lee J et al. 4-Hydroxybenzaldehyde Restricts the Intracellular Growth of Toxoplasma gondii by Inducing SIRT1-Mediated Autophagy in Macrophages. Korean J Parasitol. 58, 7-14 (2020)(PMID:32145722)
5. Kurashige T et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J Endocr Soc. 4, bvaa054 (2020)(PMID:32671315)
6. Chen C et al. Blockade of HCN2 Channels Provides Neuroprotection Against Ischemic Injury via Accelerating Autophagic Degradation in Hippocampal Neurons. Neurosci Bull. 36, 875-894 (2020)(PMID:32519067)
7. Nettesheim A et al. Autophagy in the Aging and Experimental Ocular Hypertensive Mouse Model. Invest Ophthalmol Vis Sci. 61, 31 (2020)(PMID:32797200)
8. Bosc C et al. Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites. Nat Commun. 11, 4056 (2020)(PMID:32792483)
Other(Immuno-EM)
1. Fujita N et al. Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife. 6, e23367 (2017)(PMID:28063257)
2. Eid N et al. Ethanol-Induced Mitochondrial Damage in Sertoli Cells is Associated with Parkin Overexpression and Activation of Mitophagy. Cells. 8, pii: E283 (2019)(PMID:30934625)