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抗生素嘌呤霉素是氨酰-tRNA的结构类似物，由于它能特异性地掺入新生多肽链的C端从而终止翻译，长期以来一直被用作蛋白质合成的抑制剂。基于这一特性，一种全新且速度显著更快的蛋白质标记方法已被开发出来：
在低浓度标记的脱氧胞苷-嘌呤霉素结合物存在的条件下，可通过体外翻译制备C端标记的全长蛋白[1]。
这些蛋白质已成功用于分析蛋白质-蛋白质相互作用（下拉实验、荧光相关光谱、蛋白质-蛋白质微阵列）和蛋白质-DNA相互作用（DNA微阵列）[2,3,4]。
与传统的翻译后标记方法相比，由于蛋白质表达与标记的同步进行，可避免耗时的额外纯化步骤。
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C47H69N13O16P2S (free acid)
Molecular Weight: 1166.14 g/mol (free acid)
Exact Mass: 1165.42 g/mol (free acid)
CAS#: 436083-86-2
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 0.10 mM - 0.11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 260 nm, ε 19.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
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产品组分
内容
型号
规格 储存温度
生物素-dC-嘌呤霉素
NU925BIO-01 100ul（0.1mM）
-20 °C
生物素-dC-嘌呤霉素
NU925BIO-02 500ul（0.1mM）
-20 °C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
Yoshikawa et al. (2018) Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC. Elife doi: 10.7554.
Starck et al. (2002) Puromycin oligonucleotides reveal steric restrictions for ribosome entry and multiple modes of translation inhibition. RNA 8 (7):890.
Starck et al. (2004) A general approach to detect protein expression in vivo using fluorescent puromycin conjugates. Chem. Biol. 11 (7):999.
Kawahashi et al. (2007) High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system. J. Biochem. 141 (1):19.Selected References
[1] Miyamoto-Sato et al. (2000) Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 28 (5):1176.
[2] Nemoto et al. (1999) Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Letters 462:43.
[3] Doi et al. (2002) Novel Fluorescence Labeling and High-Throughput Assay Technologies for In Vitro Analysis of Protein Interactions. Genome Research 12:487.
[4] Kawahashi et al. (2007) High-throughput fluorescence labeling of full-length cDNA products based on a reconstituted translation system. J. Biochem. 141 (1):19.
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