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细胞MeDIP试剂盒【直接采用细胞样本】
当胞嘧啶环的5-Carbon处的甲基组共价时， 产生5-甲基胞嘧啶，DNA就发生甲基化。这些甲基组投入DNA深沟中，并抑制转录。在人类DNA中，5-甲基胞嘧啶占DNA全基因组大约1.5%，主要在CpG处。0.3 至0.2 kb DNA段中CpG簇，称之为CpG岛，这些CpG岛典型性的出现在基因的第一个启动子区域者在其附近，在该处转录开始。在大量基因组DNA中，大部分CpG段都被重度甲基化。然而，在处于萌芽阶段的组织及正常体细胞的启动子中，CpG岛仍然保持未被甲基化，可以产生基因表达。当CpG岛在基因启动子区已经甲基化了，该基因的表达就被抑制。这种抑制起因于直接抑制特异转录因子结合，及间接充实甲基-CpG-结合蛋白及其相关抑制染色质重组装活动。除对基因转录的影响外，DNA甲基化还影响到染色质结构域的形成和基因组印记，该印记引用亲本基因的原特异表达式。
简单、可信和实验条件始终如一。
产品组分
|
组分内容 |
型号：A-P-2019-24(24次) |
型号：A-P-2019-48(48次) |
储存温度 |
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|
CP1 |
10X清洗液 |
28 ml | 56 ml | 室温 |
|
CP2 |
抗体缓冲液 |
15 ml | 30 ml | 室温 |
| CP3A | 预先-裂解液 | 4 ml | 6 ml | 室温 |
| CP3B | 裂解液 | 4 ml | 6 ml | 室温 |
| CP4 | Chip稀释液 | 4 ml | 6 ml | 室温 |
| CP5 | DNA释放液 | 1 ml | 4 ml | 室温 |
| CP6 | 反向缓冲液 | 1 ml | 4 ml | 室温 |
| CP7 | 结合液 | 5 ml | 8 ml | 室温 |
| CP8 | 洗脱液 | 0.6 ml | 1.2 ml | 室温 |
| NMI | Normal-Mouse IgG(1mg/ml)* | 10 ul | 20 ul | 4°C |
| 蛋白酶K（10mg/ml）* | 25 ul | 50 ul | 4°C | |
| 甲基化抗体K(1mg/ml)* | 25 ul | 50 ul | 4°C | |
| 带裙边条状8联管 | 3条 | 6条 | 4°C | |
| 粘性封口膜 | 3片 | 6片 | 室温 | |
| FC | F-spin Column* | 30个 | 50个 | 室温 |
|
FCT |
F-Collection Tube |
30个 | 50个 | 室温 |
|
操作手册 |
|
1 |
1 |
室温 |
文件资源
| 文件下载 | 文件内容 | 资源说明 |
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A-P-2019-细胞MeDIP试剂盒 | 操作手册 |
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注意事项
| 保存建议 | 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 |
| 警告 | 本品仅供科研使用，请勿用于临床与诊断。 |
FAQ
Yao Y et. al. (February 2022). HOXB9 blocks cell cycle progression to inhibit pancreatic cancer cell proliferation through the DNMT1/RBL2/c-yc axis. Cancer Lett. 533:215595.
Yuan WB et. al. (December 2021). TET1 mediated male reproductive toxicity induced by Bisphenol A through Catsper-Ca<sup>2+</sup> signaling pathway. Environ Pollut. 296:118739.
Shrivastava S et. al. (September 2021). Exosome-mediated stable epigenetic repression of HIV-1. Nat Commun. 12(1):5541.
Ceccarelli V et. al. (January 2020). Molecular mechanisms underlying eicosapentaenoic acid inhibition of HDAC1 and DNMT expression and activity in carcinoma cells. Biochim Biophys Acta Gene Regul Mech. :194481.
Li M et. al. (January 2019). Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2. Oncogene.
Mishra M et. al. (January 2018). Sirt1- A Guardian of the Development of Diabetic Retinopathy. Diabetes.
He Z et. al. (March 2017). <sup>TRIM36 hypermethylation is involved in polycyclic aromatic hydrocarbons-induced cell transformation</sup>. Environ Pollut. 225:93-103.
Arash Pooladi et. al. (September 2016). Potential Use of DOK7 Gene Methylation Level in the Peripheral Blood Cells as a Biomarker in Early Diagnosis of Sporadic Breast Cancer G3M. 14(3):4310-4317.
Yu HL et. al. (March 2015). Global DNA methylation was changed by a maternal high-lipid, high-energy diet during gestation and lactation in male adult mice liver. Br J Nutr. :1-8.
Chen J et. al. (January 2015). Association between DNA methylation and multidrug resistance in human glioma SHG-44 cells. Mol Med Rep. 11(1):43-52.
Michailidi C et. al. (April 2014). Genome-wide and gene-specific epigenomic platforms for hepatocellular carcinoma biomarker development trials. Gastroenterol Res Pract. 2014:597164.
Bellizzi D et. al. (December 2013). The control region of mitochondrial DNA shows an unusual CpG and non-CpG methylation pattern. DNA Res. 20(6):537-47.
Calabrese F et. al. (August 2013). Lack of serotonin transporter alters BDNF expression in the rat brain during early postnatal development. Mol Neurobiol. 48(1):244-56.
Lomniczi A et. al. (March 2013). Epigenetic control of female puberty. Nat Neurosci. 16(3):281-9.
Glait-Santar C et. al. (August 2012). Expression pattern of SVEP1 alternatively-spliced forms. Gene. 505(1):137-45.
Glait-Santar C et. al. (December 2011). SVEP1 promoter regulation by methylation of CpG sites. Gene. 490(1-2):6-14.
Schwarz JM et. al. (December 2011). Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression. J Neurosci. 31(49):17835-47.
Bechtel W et. al. (May 2010). Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 16(5):544-50.
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