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DNA甲基转移酶1（DNMT1）试剂盒设计用于测量组织或细胞的Dnmt1总量。在使用该试剂盒进行检测时，独特的Dnmt亲和底物被稳定地涂在条形孔上。将样品加入孔中，样品中含有的Dnmt1与底物结合。结合的Dnmt1可被特异性Dnmt1抗体识别，并通过类似ELISA的反应进行比色定量。Dnmt1的数量与显色的强度成正比。
DNA甲基转移酶1（DNMT1）试剂盒是一个方便的工具包，允许实验者以极快的速度和一致性测量Dnmt1的数量；比目前所有其他方法更优越和安全。该试剂盒是即用型的，提供了进行成功的Dnmt1检测实验所需的所有基本成分，不需要电泳和转移过程。DNA甲基转移酶1（DNMT1）试剂盒适用于定量测量人类和小鼠的新鲜组织和培养细胞中的Dnmt数量。
合作开发的DNA甲基转移酶1（DNMT1）试剂盒。具有如下的优势和特性：
- 超快的程序，可在3小时内完成。
- 创新的比色法，定量测量Dnmt1的数量，无需电泳。
- 条形微孔板格式使检测灵活：手工或高通量分析。
- 简单、可靠、一致的检测条件。
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产品组分
组分内容
型号：A-P-3011-48(48次)
型号：A-P-3011-96(96次)
储存温度
D1
10x Wash Buffer
14 ml 28 ml 4°C D2 Assay Buffer 5 ml 10 ml 4°C D3 DNMT1 Standard,20ug/ml*
20 ul 40 ul 室温 D4 Capature Antibody,500ug/ml* 8 ul 16 ul 4°C D5 Detection Antibody,200ug/ml*
10 ul 20 ul 室温 D6 Developing Solution* 6 ml 12 ml 4°C D7 Stop Solution
6 ml 11 ml 室温 BB Blocking Buffer 10 ml 20 ml 4°C 8-Well Assay Strips(With Frame)
6 12 4°C 操作手册
1
1
室温
*注意：在使用之前，为了最大限度的利用产品，请在开盖之前离心溶液至管底。在使用之前，将冷冻的试剂瓶约5~10分钟恢复到室温。
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营销中心
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注意事项
保存建议 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 警告 本品仅供科研使用，请勿用于临床与诊断。 -
FAQ
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Dong et. al. (July 2018). Effect of luteolin on the methylation status of the OPCML gene and cell growth in breast cancer cells Experimental and Therapeutic Medicine.
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Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.
Mukhopadhyay P et. al. (October 2015). Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol.
Yang G et. al. (July 2015). DNA methyltransferase 3, a target of microRNA-29c, contributes to neuronal proliferation by regulating the expression of brain-derived neurotrophic factor. Mol Med Rep. 12(1):1435-42.
Selokar NL et. al. (April 2015). Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level. Cell Reprogram. 17(2):89-94.
Das DS et. al. (December 2014). Anti-Myeloma Activity of a Novel Free Radical Inducer Rrx-001 Blood. 124(21):4712-4712.
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Wu Z et. al. (September 2013). Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus via inhibiting DNMT1 catalytic activity. J Dermatol Sci. 71(3):167-73.
Jang JY et. al. (February 2013). ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression. Int J Oncol. 42(2):574-82.
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