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EPZ015666 (GSK3235025) 是一种口服有效的 PRMT5 酶活性抑制剂，IC50为 22 nM。
生物活性
产品描述 EPZ015666 (GSK3235025) is an orally available inhibitor of PRMT5 enzymatic activity.
靶点活性 (IC₅₀) PRMT5:5 nM(Ki)
体外活性 口服200 mg/kg EPZ015666对MCL异种移植动物模型具有强的抗肿瘤活性.
体内活性 EPZ015666显示有效的细胞活性,阻断SmD3的对称二甲基化,并抑制MCL细胞系的增殖,IC50为96-904 nM。
激酶实验 EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384-well polypropylene V-bottom microplate. 3H-SAM is serially diluted twofold in assay buffer for a seven-point dilution series with a top concentration of 700 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 40 nM peptide (final assay concentrations for both). Reactions are incubated for 60 min and quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration). For the peptide competition, EPZ015666 is serially diluted threefold from 1,000 to 0.051 nM and spotted into a 384-well polypropylene V-bottom microplate. Peptide is serially diluted twofold in assay buffer for a seven-point dilution series with a top concentration of 480 nM (final assay concentration). Reactions are initiated by the addition of 4 nM enzyme and 75 nM 3H-SAM (final assay concentrations for both). Reactions are incubated for 60 min, and the reactions are quenched by the addition of 10 μL per well of 600 μM unlabeled SAM in assay buffer (final assay concentration)[1].
细胞实验 EPZ015666 is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.2%) before use[1]. Cultured cells in linear/log-phase growth are split to a seeding density of 2×105 cells/mL in 2-20 mL of media, depending on the yield required at the end of the growth period. Compound is diluted in DMSO and added to each culture vessel with a final DMSO concentration of 0.2%. Cells are allowed to grow for 96 h undisturbed. At the conclusion of each treatment period, cells are harvested by centrifugation (5 min, 1,200 rpm), and cell pellets are rinsed once with PBS before being frozen on dry ice pending further processing. Long-term proliferation assays are performed on all MCL lines, with slight adjustments to initial seeding densities, depending on growth characteristics for each cell line. All assays are carried out for 12 d[1].
别名 GSK3235025
化学信息
分子量 383.4442 分子式 C20H25N5O3 CAS No. 1616391-65-1 Smiles O[C@@H](CNC(=O)C1=NC=NC(NC2COC2)=C1)CN1CCC2=C(C1)C=CC=C2 密度 1.331 g/cm3 (Predicted) -
产品组分
内容
型号
规格 储存温度
EPZ015666 T6076-01 2mg -20°C EPZ015666 T6076-02 5mg -20°C EPZ015666 T6076-03 10mg -20°C EPZ015666 T6076-04 25mg -20°C EPZ015666 T6076-05 50mg -20°C EPZ015666 T6076-06 100mg -20°C EPZ015666 T6076-07 200mg EPZ015666 T6076-08 1mLx10mM in DMSO -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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