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DNA拓扑异构酶I是一种关键的核酶,它将超螺旋的DNA相互转换为正常DNA复制和转录所需的拓扑构象。这种酶是所谓Scl-70自身抗体的目标抗原。Scl-70抗体是硬皮病患者的特异性标志物(特异性98-100%),与弥漫性皮肤受累和肺纤维化有关。在人体组织中,DNA拓扑异构酶I最初被合成为分子量为100kD的蛋白质。这种前体的大部分随后被蛋白酶加工成分子量为70kD的物种,Scl-70抗原就是由此得名。在SF9中生产的重组人Scl-70是一个糖基化的多肽链,分子质量为91,576道尔顿。重组Scl-70用6倍His标签表达,并通过专有的色谱技术进行纯化。
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, Sf9 insect cells
Purity
≥95% (SDS-PAGE)
Concentration
~0.9mg/ml
Form
Supplied as a liquid in 16mM HEPES, pH 7.5, 400mM sodium chloride, 20% glycerol
Grade
Highly Purified
Molecular Weight
91.58
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
关键词:- 重组人DNA拓扑异构酶1(Scl-70)
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产品组分
内容
型号
规格 储存温度
重组人DNA拓扑异构酶1(Scl-70) T8065-01 5ug -20°C 重组人DNA拓扑异构酶1(Scl-70) T8065-02 20ug -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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1. A phase I study of the safety and pharmacokinetics of edotecarin (J-107088), a novel topoisomerase I inhibitor, in patients with advanced solid tumors. Cancer Chemother Pharmacol 2006 Jul 4;
2. 4-Nitroquinoline-1-Oxide Induces the Formation of Cellular Topoisomerase I-DNA Cleavage Complexes. Cancer Res 2006 Jul 1;66(13):6540-6545
3. Topoisomerase I, II{alpha} and II{beta} mRNA expression in peripheral blood mononuclear cells of patients with solid tumor: preliminary results. Ann Oncol 2006 May;17 Suppl 5:v25-v28
4. Cellular stress triggers the human topoisomerase I damage response independently of DNA damage in a p53 controlled manner. Oncogene 2006 Jun 26;
5. DNA topoisomerase I from parasitic protozoa: A potential target for chemotherapy. Biochim Biophys Acta 2006 Mar-Apr;1759(3-4):117-31
6. Evaluation of indenoisoquinoline topoisomerase I inhibitors using a hollow fiber assay. Bioorg Med Chem Lett 2006 May 31;
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