- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
PullG6测定普鲁兰酶采用了一种水溶性的定义底物，即4,6-O-亚苄基-4-硝基苯基-63-α-D-麦芽三糖（BPNPG3G3），加上辅助酶α-葡糖苷酶和β-葡糖苷酶。当底物在1,6-α连接处被普鲁兰酶或极限糊精酶水解后，释放的4-硝基苯基-β-麦芽三糖在试剂混合物中的α-葡萄糖苷酶和β-葡萄糖苷酶的协同作用下立即水解成葡萄糖和4-硝基苯酚。反应终止，加入稀碱使酚类离子显影。在400纳米处读取吸光度，得到的数值与普鲁兰特酶的活性直接相关。
支链淀粉酶/限制糊精酶分析试剂盒(PullG6法)流程图
支链淀粉酶/限制糊精酶分析试剂盒(PullG6法)具有如下优势和特性：
- 高灵敏度
- 适用于手动和自动分析仪形式
- 没有转糖基化的干扰
- 非常具有成本效益
- 所有试剂在制备后稳定时间大于1年
- 特异性很强
- 格式简单
- 包括标准品
Analyte: Pullulanase/Limit-Dextrinase Assay Format: Spectrophotometer Detection Method: Absorbance Wavelength (nm): 400 Signal Response: Increase Limit of Detection: 0.18 U/mL for pullulanase preparations (50-fold dilution)
0.01 U/g for limit dextrinase in milled maltReproducibility (%): ~ 3% Total Assay Time: ~ 10 min (Pullanase),
~ 30 min (Limit-Dextrinase)Application examples: Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. Method recognition: Novel method -
产品组分
内容
型号
规格 储存温度
支链淀粉酶/限制糊精酶分析试剂盒(PullG6法) K-PullG6 100次/200次 2-8°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Diastatic power and maltose value: a method for the measurement of amylolytic enzymes in malt.
Charmier, L. M., McLoughlin, C. & McCleary, B. V. (2021). Journal of the Institute of Brewing, In Press.
Prediction of potential malt extract and beer filterability using conventional and novel malt assays.
Cornaggia, C., Evans, D. E., Draga, A., Mangan, D. & McCleary, B. V. (2019). Journal of Institute of Brewing, 125(3), 294-309.
Colourimetric and fluorimetric substrates for the assay of limit dextrinase.
Mangan, D., McCleary, B. V., Cornaggia, C., Ivory, R., Rooney, E. & McKie, V. (2015). Journal of Cereal Science, 62, 50-57.
Colourimetric and fluorometric substrates for measurement of pullulanase activity.
McCleary, B. V., Mangan, D., McKie, V., Cornaggia, C., Ivory, R. & Rooney, E. (2014). Carbohydrate Research, 393, 60-69.
A quick and simple gel diffusion assay to visualize and quantify pullulanase activity for resistant starch content in food crops.
Krishnan, V., Awana, M., Kulshreshta, A., Praveen, S. & Singh, A. (2022). Journal of Plant Biochemistry and Biotechnology, 1-7.
Effects of post-heading high temperature on some quality traits of malt barley.
Ni, S. J., Zhao, H. F. & Zhang, G. P. (2020). Journal of Integrative Agriculture, 19(11), 2674-2679.
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
