- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
来源: Rabbit 应用: WB IHC IF/ICC IP CHIP 反应: Human, Mouse, Rat 分子量: 15kDa; 16kD(Calculated). 蛋白号: Q16695 RRID: AB_2838900 来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Tri-Methyl-Histone H3 (Lys27)/H3K27me3 Antibody detects endogenous levels of Tri-Methyl-Histone H3 only when methylated at Lys27.
RRID:
AB_2838900
引用格式: Affinity Biosciences Cat# DF6941, RRID:AB_2838900.偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
-
产品组分
内容
型号
规格 储存温度
组蛋白H3K27me3抗体 DF6941-01 50ul -20°C 组蛋白H3K27me3抗体 DF6941-02 100ul -20°C 组蛋白H3K27me3抗体 DF6941-03 200ul -20°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
1). Li T et al. P21 and P27 promote tumorigenesis and progression via cell cycle acceleration in seminal vesicles of TRAMP mice. Int J Biol Sci 2019 Aug 19;15(10):2198-2210 (PubMed: 31592235) [IF=10.750]
2). Ding ZM et al. Triclocarban exposure affects mouse oocyte in vitro maturation through inducing mitochondrial dysfunction and oxidative stress. Environ Pollut 2020 Feb 25;262:114271 (PubMed: 32135433) [IF=9.988]
Application: IF/ICC Species: mouse Sample: oocytes
Fig. 8. TCC exposure altered epigenetic modification in mouse oocytes. (A) Images depicting H3K27me2 in control and TCC-treated oocytes. H3K27me2, red. Bar, 10 mm. (B) The fluorescence intensity of H3K27me2 in control and TCC- treated oocytes. Control, n ¼ 20; 12 mM, n ¼ 19. ***Significantly different (P < 0.0001). (C) Images depicting H3K27me3 in control and TCC-treated oocytes. H3K27me2, red. Bar, 10 mm. (D) The fluorescence intensity of H3K27me3 in control and TCC- treated oocytes. Control, n ¼ 30; 12 mM, n ¼ 24. ***Significantly different (P < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
3). Chen SL et al. HBx-mediated decrease of AIM2 contributes to hepatocellular carcinoma metastasis. Mol Oncol 2017 Sep;11(9):1225-1240 (PubMed: 28580773) [IF=7.449]
Application: WB Species: Sample:
Figure 3. EZH2 is required for HBx-induced AIM2 decrease. A. AIM2 mRNA and protein expression levels were tested in Bel-7402 and SMMC-7721 cells treated with 5-Aza for 24 h. B. EZH2 and H3K27me3 level was detected in Bel-7402 and SMMC-7721 cells transfected with HBx. C. EZH2 was transiently knocked down by siRNAs in HBx-expressing Bel-7402 and SMMC-7721 cells. AIM2 expression was determined. D. EZH2 activity was blocked by its inhibitor GSK-126 in cells with or without the presence of HBx. The impact of EZH2 on AIM2 expression was checked.
4). Zhang SX et al. Bisphenol B exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment. Front Cell Dev Biol 2020 Dec 17;8:616771. (PubMed: 33392205) [IF=6.081]
Application: IF/ICC Species: mouse Sample: oocytes
FIGURE 7 | BPB exposure altered epigenetic modification in mouse oocytes.(A) Images depicting H3K27me3 in control and BPB-treated oocytes.H3K27me3, red. Bar, 10 µm.
5). Ding ZM et al. Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage. Environ Toxicol 2022 Feb 26. (PubMed: 35218298) [IF=4.109]
6). Jiao XF et al. Effects of Acute Fluorene-9-Bisphenol Exposure on Mouse Oocyte in vitro Maturation and Its Possible Mechanisms. Environ Mol Mutagen 2018 Nov 30 (PubMed: 30499614) [IF=3.579]
Application: IF/ICC Species: mouse Sample: oocytes
Fig. 6. |BHPF exposure alters H3K9me3 and H3K27me3 levels in mouse oocytes. (A) Immunofluorescent staining for H3K9me3 in control and BHPF-treated oocytes. H3K9me3 level was significantly higher in treated oocytes. Red, H3K9me3; Blue, DAPI. Scale bar, 5 μm.(B) Average H3K9me3 fluorescence intensity in control and BHPF-treated mouse oocytes. (C) Immunofluorescent staining for H3K27me3 in control and BHPF-treated oocytes. H3K27me3 level was significantly higher in treated oocytes. Red, H3K27me3; Blue, DAPI. Scale bar, 5 μm (D) Average H3K27me3 fluorescence intensity in control and BHPF-treated mouse oocytes. **P < 0.01.
7). Meng F et al. Isobutylparaben negatively affects porcine oocyte maturation through increasing oxidative stress and cytoskeletal abnormalities. Environ Mol Mutagen 2020 Jan 10 (PubMed: 31922297) [IF=3.579]
Application: IF/ICC Species: Pig Sample: Cumulus cells
Fig. 6. IBP exposure alters H3K9me3 and H3K27me3 levels in porcine oocytes. (A) Immunofluorescent staining of H3K9me3 and H3K27me3 in control and IBP exposure group. The histone methylation level was significantly higher in treated oocytes. H3K9me3 and H3K27me3; Blue,DAPI. (B) Average H3K9me3 fluorescent intensity of H3K9me3 in control and IBP-treated oocytes. (C) Average fluorescent intensity of H3K27me3 in control and IBP-treated oocytes. **P < 0.01; ***P < 0.001; scale bar = 50 μm. DIC, differential interference contrast.
猜你喜欢
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
