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Application: DB, ELISA, IF, IHC, IP, MeDIP Areas of Research: DNA Methylation Clonality: Monoclonal Conjugate: Unconjugated Host: Mouse Isotype: IgG1 Purification: Affinity Purified Reactivity: Broad Range Trial Size Available: Yes Background
5-methylcytosine (5-mC) is formed when DNA methyltransferase (DNMT) catalyzes the addition of a methyl group onto the 5-carbon of the cytosine ring, an epigenetic process known as DNA methylation. 5-mC is considered the "fifth" DNA base and this 5-methylcytosine mouse monoclonal antibody is ideal for discriminating between the unmodified cytosine base (C) and the methylated cytosine base (5-mC) for DNA methylation studies. DNA methylation, the major epigenetic modification of eukaryotic genomes, plays an essential role in mammalian development. DNA methylation of promoter regions leads to inactivation of gene function. Also, DNA methylation status varies according to tissue type, and region-specific DNA hypermethylation and global DNA hypomethylation have been demonstrated to play an important role in tumorigenesis.Description
Mouse monoclonal antibody to 5-methylcytosine (5-mC), clone 33D3, MeDIP/ChIP-grade, used in DNA methylation studies.Concentration
1 mg/mlPurification
Protein AImmunogen
Ovalbumin-conjugated 5-methylcytosine (5-mC)Isotype
IgG1Specificity
Modified base 5-methylcytosine (5-mC), a broad range of species.Formulation
Purified IgG in 10 mM phosphate buffer, 150 mM NaCl, pH 7.4.Storage
4°C, stable for 6 months from the date of shipment. For long-term storage, aliquot and store at -20°C. Avoid repeated freezing and thawing. Multiple freeze/thaw cycles may result in decreased performance.Handling Recommendations
For maximum recovery of the products, centrifuge the vial prior to opening the cap.Alternative Names
5-methylcytidine, anti-5-methylcytidine, anti-5-methylcytosine, anti-5mC, anti-5-mC, anti-5meC, anti-5-meC, 5mC, 5meC, 5-meC, 5'-methyl-2'-deoxycytidine, 5MedCydApplication & Suggested Dilutions*
Dot Blot: 1:1000-1:2000; Immunohistochemistry: 1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:1000-1:2000; MeDIP: 0.5-1 µg/reaction*The end user is responsible for determining optimal working dilutions.
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5-甲基胞嘧啶(5-mC)鼠源单克隆抗体(33D3) A-1014-010 10ug -20°C 5-甲基胞嘧啶(5-mC)鼠源单克隆抗体(33D3) A-1014-050 50ug -20°C 5-甲基胞嘧啶(5-mC)鼠源单克隆抗体(33D3) A-1014-100 100ug -20°C 操作手册
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A-1014-5-甲基胞嘧啶(5-mC)鼠源单克隆抗体(33D3) 操作手册 A-1014-5-甲基胞嘧啶(5-mC)鼠源单克隆抗体(33D3)相关说明 宣传单页 -
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