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RhoA G-LISA激活检测试剂盒(比色法)是一种基于 ELISA 的 Rho 活化检测试剂盒，可在 3 小时内检测细胞中的 Rho 活性。有关 G-LISA 检测法的详细介绍以及其他可用的 G-LISA 试剂盒列表，请参阅我们的 G-LISA 主页。BK124 Rho 活化检测试剂盒仅检测细胞中 GTP 负载的 RhoA 水平。活化水平在吸光度为 490nm 时测定。
RhoA G-LISA激活检测试剂盒(比色法)实验的流程图
图 1. 用 RhoA G-LISA激活检测试剂盒(比色法)BK124 测量钙泊蛋白对 RhoA 的激活作用。瑞士 3T3（小鼠）细胞血清饥饿 24 小时，然后用钙蛋白肽（Cal；0.1 毫克/毫升，30 分钟）或仅用 DMSO 载体（SS）处理。对 10 µg 细胞裂解液进行 G-LISA™ 检测。在 490 纳米波长处读取吸光度。
图 2. 使用RhoA G-LISA激活检测试剂盒(比色法)（Cat.# BK124）测量经细胞渗透性 Rho 抑制剂（CT04）处理的瑞士 3T3 细胞的 Rho 活性。血清饥饿的瑞士 3T3 成纤维细胞未经处理（无 CT04），或在 37°C 无血清培养基中用 0.20、0.50 和 2.0 µg/ml CT04 处理 4 小时，然后用 100µg/ml 卡尔蛋白酶活化 10 分钟。 然后裂解细胞，用 RhoA G-LISA激活检测试剂盒(比色法)（Cat.# BK124）检测 RhoA 活性。 注：2.0 µg/ml CT04 作用 4 小时后，RhoA 活性几乎完全（90%）被抑制。
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RhoA G-LISA激活检测试剂盒(比色法)
CNBK124-01 24次 2-8°C RhoA G-LISA激活检测试剂盒(比色法)
CNBK124-02 96次 2-8°C 操作手册
1 1 常温
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Author Title Journal Year Article Link Anastasaki, Corina et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization STAR Protocols 2022 ISSN 2666--1667 Vadakumchery, Anila et al. The Small GTPase RHOA Links SLP65 Activation to PTEN Function in Pre B Cells and Is Essential for the Generation and Survival of Normal and Malignant B Cells Frontiers in Immunology 2022 ISSN 1664-3224 Zanin, Juan P. et al. p75NTR prevents the onset of cerebellar granule cell migration via RhoA activation eLife 2022 ISSN 2050-084X Nath, Anu S. et al. Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses Cell Reports 2022 ISSN 2211-1247 Kumar, Akhilesh et al. Essential role of Rnd1 in innate immunity during viral and bacterial infections Cell Death & Disease 2022 Article Link Francis, Caitlin R. et al. Rab35 governs apicobasal polarity through regulation of actin dynamics during sprouting angiogenesis Nature Communications 2022 13:1 2022 ISSN 2041--1723 Xue, Tan et al. Effects of Aster B-mediated intracellular accumulation of cholesterol on inflammatory process and myocardial cells in acute myocardial infarction Hellenic Journal of Cardiology 2022 Hauke, Michael et al. Active RhoA Exerts an Inhibitory Effect on the Homeostasis and Angiogenic Capacity of Human Endothelial Cells Journal of the American Heart Association 2022 ISSN 2047-9980 Darp, Revati et al. Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesis Nature Communications 2022 13:1 2022 ISSN 2041--1723 Meng, Zhipeng et al. The Hippo pathway mediates Semaphorin signaling Science Advances 2022 ISSN 2375-2548 de Vallière, Cheryl et al. pH-Sensing G Protein-Coupled Receptor OGR1 (GPR68) Expression and Activation Increases in Intestinal Inflammation and Fibrosis International Journal of Molecular Sciences 2022 ISSN 1422-0067 Wang, Kankai et al. PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor Theranostics 2022 ISSN 1838-7640 Kumar, Akhilesh et al. Essential role of Rnd1 in innate immunity during viral and bacterial infections Cell Death & Disease 2022 13:6 2022 ISSN 2041--4889 Yadav, Vikas et al. Increased MARCKS Activity in BRAF Inhibitor-Resistant Melanoma Cells Is Essential for Their Enhanced Metastatic Behavior Independent of Elevated WNT5A and IL-6 Signaling Cancers 2022 ISSN 2072-6694 Weder, Bruce et al. New Therapeutic Approach for Intestinal Fibrosis Through Inhibition of pH-Sensing Receptor GPR4 Inflammatory Bowel Diseases 2022 ISSN 1078--0998 Sánchez-de la Torre, Aníbal et al. Cannabinoid CB1 receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice Cell Death & Disease 2022 13:7 2022 ISSN 2041--4889 Ma, Yuanyuan et al. Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root development Development (Cambridge) 2021 ISSN 1477-9129 Zhou, Qun et al. Inflammatory Immune Cytokine TNF-α Modulates Ezrin Protein Activation via FAK/RhoA Signaling Pathway in PMVECs Hyperpermeability Frontiers in Pharmacology 2021 ISSN 1663-9812 Jozic, Ivan et al. Glucocorticoid-mediated induction of caveolin-1 disrupts cytoskeletal organization, inhibits cell migration and re-epithelialization of non-healing wounds Communications Biology 2021 ISSN 2399-3642 Gurusamy, Malarvizhi et al. G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators Nature Communications 2021 ISSN 2041-1723 Shoda, Tetsuo et al. Desmoplakin and periplakin genetically and functionally contribute to eosinophilic esophagitis Nature Communications 2021 ISSN 2041-1723 Krueger, Irena et al. Reelin amplifies glycoprotein VI activation and alphaiib beta3 integrin outside-in signaling via PLC Gamma 2 and Rho GTPases Arteriosclerosis, Thrombosis, and Vascular Biology 2020 ISSN 1524-4636 Salgado-Lucio, Monica L. et al. FAK regulates actin polymerization during sperm capacitation via the ERK2/GEF-H1/RhoA signaling pathway Journal of Cell Science 2020 ISSN 1477-9137 Lachowski, Dariusz et al. G Protein-Coupled Estrogen Receptor Regulates Actin Cytoskeleton Dynamics to Impair Cell Polarization Frontiers in Cell and Developmental Biology 2020 ISSN 2296-634X Rong, Zhouyi et al. Activation of FAK/Rac1/Cdc42-GTPase signaling ameliorates impaired microglial migration response to Aβ42 in triggering receptor expressed on myeloid cells 2 loss-of-function murine models FASEB Journal 2020 ISSN 1530-6860 Fox, Megan E. et al. Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior Molecular Psychiatry 2020 ISSN 1476-5578 Hasan, Wan Nuraini Wan et al. Annatto-derived tocotrienol promotes mineralization of MC3T3-E1 cells by enhancing BMP-2 protein expression via inhibiting RhoA activation and HMG-CoA reductase gene expression Drug Design, Development and Therapy 2020 ISSN 1177-8881 Bhosle, Vikrant K. et al. SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling Nature Communications 2020 ISSN 2041-1723 Porter, Lauren et al. SUN1/2 Are Essential for RhoA/ROCK-Regulated Actomyosin Activity in Isolated Vascular Smooth Muscle Cells Cells 2020 ISSN 2073-4409 Dias Gomes, Martim et al. Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity Nature Communications 2019 ISSN 2041-1723 Majolée, Jisca et al. CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrity Scientific Reports 2019 ISSN 2045-2322 Santhana Kumar, Karthiga et al. TGF-β Determines the Pro-migratory Potential of bFGF Signaling in Medulloblastoma Cell Reports 2018 ISSN 2211-1247 Li, Xu et al. A positive feedback loop of profilin-1 and RhoA/ROCK1 promotes endothelial dysfunction and oxidative stress Oxidative Medicine and Cellular Longevity 2018 ISSN 1942-0994 Schillaci, Odessa et al. Exosomes from metastatic cancer cells transfer amoeboid phenotype to non-metastatic cells and increase endothelial permeability: Their emerging role in tumor heterogeneity Scientific Reports 2017 ISSN 2045-2322 Rom, Slava et al. PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier Journal of Neuroinflammation 2016 ISSN 1742-2094 Jackson, William D. et al. Very-low and low-density lipoproteins induce neutral lipid accumulation and impair migration in monocyte subsets Scientific Reports 2016 ISSN 2045-2322 López-Posadas, Rocío et al. Rho-A prenylation and signaling link epithelial homeostasis to intestinal inflammation Journal of Clinical Investigation 2016 ISSN 1558-8238 Yu, Yonghao et al. Hydrogen-rich medium ameliorates lipopolysaccharide-induced barrier dysfunction via rhoa-mdia1 signaling in caco-2 cells Shock 2016 ISSN 1540-0514 Patra, Vijay Kumar et al. The Skin Microbiome: Is It Affected by UV-induced Immune Suppression? Frontiers in Microbiology 2016 ISSN 1664-302X Lu, Wen Juan et al. Senescence Mediated by p16INK4a Impedes Reprogramming of Human Corneal Endothelial Cells into Neural Crest Progenitors Scientific Reports 2016 ISSN 2045-2322 Rom, Slava et al. The dual action of poly(ADP-ribose) polymerase -1 (PARP-1) inhibition in HIV-1 infection: HIV-1 ltr inhibition and diminution in Rho GTPase activity Frontiers in Microbiology 2015 ISSN 1664-302X Ochoa-Alvarez, Jhon A. et al. Antibody and lectin target podoplanin to inhibit oral squamous carcinoma cell migration and viability by distinct mechanisms Oncotarget 2015 ISSN 1949-2553 Aifuwa, Ivie et al. Senescent stromal cells induce cancer cell migration via inhibition of RhoA/ROCK/myosin-based cell contractility Oncotarget 2015 ISSN 1949-2553 Freeman, Spencer A. et al. Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor Nature Communications 2015 ISSN 2041-1723 Tang, Xiao et al. HCLOCK Causes Rho-Kinase-Mediated Endothelial Dysfunction and NF-κ B-Mediated Inflammatory Responses Oxidative Medicine and Cellular Longevity 2015 ISSN 1942-0994 Skrbic, Biljana et al. Lack of collagen VIII reduces fibrosis and promotes early mortality and cardiac dilatation in pressure overload in mice Cardiovascular Research 2015 ISSN 1755-3245 Manukyan, Arkadi et al. A complex of p190RhoGAP-A and anillin modulates RhoA-GTP and the cytokinetic furrow in human cells Journal of Cell Science 2015 ISSN 1477-9137 Rom, Slava et al. Poly(ADP-ribose) polymerase-1 inhibition in brain endothelium protects the blood-brain barrier under physiologic and neuroinflammatory conditions Journal of Cerebral Blood Flow and Metabolism 2015 ISSN 1559-7016 Biechler V., Stefanie V. et al. The impact of flow-induced forces on the morphogenesis of the outflow tract Frontiers in Physiology 2014 ISSN 1664-042X Mackay, Joanna L. et al. Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters Integrative Biology (United Kingdom) 2014 ISSN 1757-9708 Chen, Xiaofei et al. The TMEFF2 tumor suppressor modulates integrin expression, RhoA activation and migration of prostate cancer cells Biochimica et Biophysica Acta - Molecular Cell Research 2014 ISSN 1879-2596 Herr, Michael J. et al. Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cells PLoS ONE 2014 ISSN 1932-6203 Zhu, Ying Ting et al. Knockdown of both p120 catenin and kaiso promotes expansion of human corneal endothelial monolayers via rhoa-rock-noncanonical BMP-NFκB pathway Investigative Ophthalmology and Visual Science 2014 ISSN 1552-5783 Dubash, Adi D. et al. The GEF Bcr activates RhoA/MAL signaling to promote keratinocyte differentiation via desmoglein-1 Journal of Cell Biology 2013 ISSN 0021-9525 DiScipio, Richard G. et al. Complement C3a signaling mediates production of angiogenic factors in mesenchymal stem cells Journal of Biomedical Science and Engineering 2013 ISSN 1937--6871 Rom, Slava et al. Selective activation of cannabinoid receptor 2 in leukocytes suppresses their engagement of the brain endothelium and protects the blood-brain barrier American Journal of Pathology 2013 ISSN 0002-9440 Yang, Jian et al. Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo International Journal of Biochemistry and Cell Biology 2013 ISSN 1357-2725 Papke, Christina L. et al. Smooth muscle hyperplasia due to loss of smooth muscle α-actin is driven by activation of focal adhesion kinase, altered p53 localization and increased levels of platelet-derived growth factor receptor-β Human Molecular Genetics 2013 ISSN 0964-6906 Tan, Hong et al. Fluid flow forces and rhoA regulate fibrous development of the atrioventricular valves Developmental Biology 2013 ISSN 1095-564X Kalia, Manjula et al. Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism Journal of Virology 2013 ISSN 0022--538X Kanazawa, Yasushi et al. The Rho-kinase inhibitor fasudil restores normal motor nerve conduction velocity in diabetic rats by assuring the proper localization of adhesion-related molecules in myelinating Schwann cells Experimental neurology 2013 ISSN 1090--2430 Zhou, Zhigang et al. HSV-mediated gene transfer of C3 transferase inhibits Rho to promote axonal regeneration Experimental Neurology 2012 ISSN 0014--4886 Ramsay, Alan G. et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenali******: Establishing a reversible immune evasion mechanism in human cancer Blood 2012 ISSN 1528-0020 Chen, Si Meng et al. Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR Biochemical Pharmacology 2012 ISSN 0006-2952 Takefuji, Mikito et al. G13-mediated signaling pathway is required for pressure overload-induced cardiac remodeling and heart failure Circulation 2012 ISSN 0009-7322 Howe, Grant A. et al. RhoB controls endothelial cell morphogenesis in part via negative regulation of RhoA Vascular Cell 2012 ISSN 2045--824X Zhu, Ying Ting et al. Nuclear p120 catenin unlocks mitotic block of contactinhibited human corneal endothelial monolayers without disrupting adherent junctions Journal of Cell Science 2012 ISSN 0021-9533 Yang, Seungwon et al. The RhoA-ROCK-PTEN pathway as a molecular switch for anchorage dependent cell behavior Biomaterials 2012 ISSN 1878--5905 McCoy, Kelly L. et al. Protease-activated receptor 1 (PAR1) coupling to G(q/11) but not to G(i/o) or G(12/13) is mediated by discrete amino acids within the receptor second intracellular loop Cellular signalling 2012 ISSN 1873--3913 Ramseyer, Vanesa D. et al. Tumor necrosis factor α decreases nitric oxide synthase type 3 expression primarily via Rho/Rho kinase in the thick ascending limb Hypertension (Dallas, Tex. : 1979) 2012 ISSN 1524--4563 Dhaliwal, Anandika et al. Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases 2012 PMID 22509380 Greco, Carolina M. et al. Chemotactic effect of prorenin on human aortic smooth muscle cells: a novel function of the (pro)renin receptor Cardiovascular Research 2012 ISSN 0008--6363 Chen, Guang et al. Inhibition of chemokine (CXC motif) ligand 12/chemokine (CXC motif) receptor 4 axis (CXCL12/CXCR4)-mediated cell migration by targeting mammalian target of rapamycin (mTOR) pathway in human gastric carcinoma cells (Journal of Biological Chemistry (2012) 2 Journal of Biological Chemistry 2012 ISSN 0021-9258 Elali, Ayman et al. Liver X receptor activation enhances blood-brain barrier integrity in the ischemic brain and increases the abundance of ATP-binding cassette transporters ABCB1 and ABCC1 on brain capillary cells Brain Pathology 2012 ISSN 1015-6305 Garrido‐Gómez, Tamara et al. Annexin A2 is critical for embryo adhesiveness to the human endometrium by RhoA activation through F‐actin regulation The FASEB Journal 2012 ISSN 0892--6638 Ganguly, Riya et al. Adiponectin Increases LPL Activity via RhoA/ROCK-Mediated Actin Remodelling in Adult Rat Cardiomyocytes Endocrinology 2011 ISSN 0013--7227 Mammoto, Tadanori et al. Mechanochemical Control of Mesenchymal Condensation and Embryonic Tooth Organ Formation Developmental Cell 2011 ISSN 1534-5807 Aguilar, Hector N. et al. Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes PLoS ONE 2011 ISSN 1932-6203 Jin, Wanzhu et al. Increased SRF transcriptional activity in human and mouse skeletal muscle is a signature of insulin resistance Journal of Clinical Investigation 2011 ISSN 0021-9738 Musso, Alessandra et al. Relevance of the mevalonate biosynthetic pathway in the regulation of bone marrow mesenchymal stromal cell-mediated effects on T-cell proliferation and B-cell survival Haematologica 2011 ISSN 1592--8721 Ridgway, Lon D. et al. Modulation of GEF-H1 Induced Signaling by Heparanase in Brain Metastatic Melanoma Cells Journal of cellular biochemistry 2010 ISSN 0730-2312 Rapier, Rebecca et al. The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells Cancer Cell International 2010 ISSN 1475-2867 Lichtenstein, Mathieu P. et al. Secretase-independent and RhoGTPase/PAK/ERK-dependent regulation of cytoskeleton dynamics in astrocytes by NSAIDs and derivatives Journal of Alzheimer's disease : JAD 2010 ISSN 1875--8908 Yang, Enyue et al. Fluoride induces vascular contraction through activation of RhoA/Rho kinase pathway in isolated rat aortas Environmental toxicology and pharmacology 2010 ISSN 1872--7077 Nini, Lylia et al. Accurate and reproducible measurements of RhoA activation in small samples of primary cells Analytical biochemistry 2010 ISSN 1096--0309 Romero, Ana M. et al. Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary culture Toxicological Sciences 2010 ISSN 1096-6080 Chastre, Eric et al. TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness The FASEB Journal 2009 ISSN 1530--6860 Hammar, Eva et al. Role of the Rho-ROCK (Rho-Associated Kinase) Signaling Pathway in the Regulation of Pancreatic β-Cell Function Endocrinology 2009 ISSN 0013--7227 Seifert, Jennifer L. et al. Differential activation of Rac1 and RhoA in neuroblastoma cell fractions Neuroscience letters 2009 ISSN 0304--3940 Mercer, Jason et al. Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells Science (New York, N.Y.) 2008 ISSN 1095--9203 Kinoshita, Nagatoki et al. Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation Molecular Biology of the Cell 2008 ISSN 1059-1524 Korobova, Farida et al. Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells Molecular biology of the cell 2008 ISSN 1939--4586 Ramirez, Servio H. et al. Activation of Peroxisome Proliferator-Activated Receptor γ (PPARγ) Suppresses Rho GTPases in Human Brain Microvascular Endothelial Cells and Inhibits Adhesion and Transendothelial Migration of HIV-1 Infected Monocytes The Journal of Immunology 2008 ISSN 0022--1767 Moore, Simon W. et al. Rho inhibition recruits DCC to the neuronal plasma membrane and enhances axon chemoattraction to netrin 1 Development 2008 ISSN 0950--1991 Sequeira, Linda et al. Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapedesis Clinical & experimental metastasis 2008 ISSN 0262--0898 Schreibelt, Gerty et al. Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 ISSN 1530--6860 Rupp, Paul A. et al. A role for RhoA in the two-phase migratory pattern of post-otic neural crest cells Developmental Biology 2007 ISSN 0012-1606 Keely, Patricia J. et al. Investigating integrin regulation and signaling events in three-dimensional systems Methods in enzymology 2007 ISSN 0076--6879 Tanaka, Shigeru et al. Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth The Journal of biological chemistry 2007 ISSN 0021--9258 Bradley, William D. et al. Integrin Signaling through Arg Activates p190RhoGAP by Promoting Its Binding to p120RasGAP and Recruitment to the Membrane Molecular Biology of the Cell 2006 ISSN 1059-1524
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