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染色质免疫沉淀（ChIP）在技术上具有挑战性，并且结果难以解读。我们的酶法染色质免疫沉淀试剂盒通过应用蛋白质G偶联磁珠的简化方案来消除其中的一些挑战，使ChIP只需一天就能完成。试剂盒提供超声或者酶消化两种方案制备染色质。使用我们提供的染色质对照试剂盒和qPCR引物组引物组和已剪切好的染色质来完成您的实验设计，可以在每一步验证您的结果。要了解这些功能与我们提供的其他ChIP试剂盒的差异，请参阅我们的ChIP试剂盒选择指南。
ChIP涉及免疫沉淀经过交联固定的蛋白/DNA复合物。首先，完整的细胞用甲醛固定交联，从而保持蛋白和DNA的相互作用。然后，DNA被剪切成均匀的小片段，使用目标DNA结合蛋白的抗体免疫沉淀DNA/蛋白复合物。免疫沉淀后，DNA经过洗涤，解交联，蛋白被蛋白酶K处理去除。洗脱后的DNA可以用我们的染色体免疫沉淀DNA纯化试剂盒进行纯化，纯化后的DNA可以直接进行qPCR或NGS测序下游分析。
图1：使用酶法染色质免疫沉淀试剂盒示意图可提供高质量的ChIP-Seq数据
使用3µg 我们的AbFlex®组蛋白H3K9ac重组抗体（目录号91103）和的酶法染色质免疫沉淀试剂盒25µg来自K-562细胞的染色质。在ChIP之后，使用下一代DNA文库建库试剂盒（目录号53216和53264）制备Illumina兼容测序文库，并使用NextSeq 500进行测序。结果显示高质量的ChIP-Seq信号峰横跨1号染色体的一个区域。
图2：使用酶法染色质免疫沉淀试剂盒免疫沉淀组蛋白修饰H3K9ac的ChIP-Seq数据 使用酶法染色质免疫沉淀试剂盒富集组蛋白修饰H3K9ac的ChIP qPCR数据
使用3µg 我们的AbFlex®组蛋白修饰H3K9ac重组抗体（货号91103）和酶法染色质免疫沉淀试剂盒免疫沉淀25µg K-562细胞染色质。ChIP后，DNA按1:20稀释，使用我们的人阳性对照引物组ACTB-1和GAPDH-1（货号号71003和71004），人阴性对照引物组1（货号71001）进行qPCR。结果以每1000个细胞检测到的结合情况展示，结果显示阳性对照PCR引物有富集，而阴性对照引物几乎没有富集。
图3：使用酶法染色质免疫沉淀试剂盒免疫沉淀组蛋白修饰H3K9ac的ChIP-qPCR数据
酶法染色质免疫沉淀试剂盒具有如下优势和特性：
- ChIP-IT Express超声法对于难以裂解的细胞、培养细胞和组织样品来说是高质量的染色质片段化方案。
- 如无超声仪，培养细胞系样本只需简单的使用我们的ChIP-IT Express Enzymatic kit。
- 快速的操作步骤使ChIP实验在一天内完成成为可能。
- 蛋白质G磁珠提供低背景富集和简化的洗涤步骤。
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超声法染色质免疫沉淀试剂盒
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