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噬菌体DNA提取试剂盒(离心柱)提供一种快速柱式法，可从在液体培养物中生长的细菌中繁殖的多种噬菌体中纯化总DNA。DNA 的分离无需使用苯酚、氯仿或氯化铯带状程序。基于离心柱的程序非常快速，可在 45 分钟内完成。该试剂盒可高效处理少量噬菌体上清液（500 µL - 1 mL），通过可选的 DNase 和蛋白酶 K 处理，可最大限度地提高噬菌体 DNA 产量，同时将宿主 DNA 污染降至最低。纯化的总噬菌体 DNA 具有最高的完整性，可用于多种下游应用，包括 PCR、qPCR、限制性片段长度多态性 (RFLP)、测序、克隆、Southern 印迹等。
Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µL Size of DNA Purified All sizes Maximum Amount of Starting Material 1 x 1010 pfu/mL enriched phages Average Yield* 3-15 µg DNA from 106-1010 pfu/mL
of enriched phagesTime to Complete 10 Purifications 45 minutes 噬菌体DNA提取试剂盒(离心柱)具有如下优势和特性：
- 从多种噬菌体菌株中分离高质量 DNA
- 总 DNA 产量高
- 采用快速离心柱格式，处理简单快捷
- 无需酚或氯仿提取，也无需氯化铯标记
- 从 106-1010 pfu/ mL 富集噬菌体中回收 3-15 µg DNA 的高产率
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产品组分
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规格 储存温度
噬菌体DNA提取试剂盒(离心柱)
CN46800-01 50次
室温
噬菌体DNA提取试剂盒(离心柱)
CN46800-02 100次
室温
操作手册
1 1 常温
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保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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