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G-LISA 系列小 G 蛋白活化检测试剂盒是基于 ELISA 法的检测试剂盒,可在 3 小时内从细胞或组织裂解液中检测出小 G 蛋白的 GTP 形式。有关Rac1-2-3激活分析试剂盒(比色法)的更详细介绍以及其他可用的 G-LISA™ 试剂盒列表,请参阅我们的 G-LISA™ 主页。Rac1-2-3激活分析试剂盒(比色法)可检测细胞裂解液中 GTP 负载的 Rac1、2 和 3 蛋白的整体水平,这与只检测 Rac1 活化水平的型号:CNBK126 不同。活化水平是通过 490nm 波长下的吸光度测量的。如需检测 RhoA 活化的试剂盒,请查看型号:CNBK124。
Rac1-2-3激活分析试剂盒(比色法)灵敏度非常高,重复样品的准确性也非常好。更多详情,请参阅 G-LISA™ 页面上的 G-LISA™ 常见问题标签。
图 1. 用Rac1-2-3激活分析试剂盒(比色法)测量 EGF 对 Rac 的激活作用。瑞士 3T3(小鼠)细胞血清饥饿 24 小时,然后用 EGF(Cal;10ng/ml,3 分钟)或仅用缓冲液(SS)处理。对 10 µg 细胞裂解液进行 G-LISA™ 检测。在 490 纳米波长处读取吸光度。
图 2. 用Rac1-2-3激活分析试剂盒(比色法)测量 EGF 对 Rac 的激活作用。 瑞士 3T3 细胞血清饥饿(SS)24 小时,并用 EGF(10 毫微克/毫升,2 分钟)处理。对 20、10、5、2.5、1.25 µg 细胞裂解液进行 G-LISA™ 检测。在 490 纳米波长处读取吸光度。对 500 µg 的相同裂解液进行传统的 PAK 拉取检测(如插图所示,Cat.# BK035)。Rac1-2-3激活分析试剂盒(比色法)具有如下优势和特性:
- Rac 信号通路研究
- 使用原代细胞进行 Rac 激活试验
- Rac 激活剂和失活剂研究
- 利用有限的材料进行 Rac 激活测定
- 高通量筛选 Rac 激活
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型号
规格 储存温度
Rac1-2-3激活分析试剂盒(比色法) BK125 96次 2-8°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
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Author Title Journal Year Article Link Barbakadze, Tamar et al. 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin Cell Journal (Yakhteh) 2023 ISSN 2228-5814 Talwar, Shefali et al. Mechanosensitive smooth muscle cell phenotypic plasticity emerging from a null state and the balance between Rac and Rho Cell Reports 2021 ISSN 2211-1247 Lagresle-Peyrou, Chantal et al. A gain-of-function RAC2 mutation is associated with bone marrow hypoplasia and an autosomal dominant form of severe combined immunodeficiency Haematologica 2021 ISSN 1592-8721 Talamás-Lara, Daniel et al. Entamoeba histolytica and Entamoeba dispar: Morphological and Behavioral Differences Induced by Fibronectin through GTPases Activation and Actin-Binding Proteins The Journal of eukaryotic microbiology 2020 ISSN 1550--7408 Cash, Jennifer N. et al. Discovery of small molecules that target the phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent Rac exchanger 1 (P-Rex1) PIP3-binding site and inhibit p-Rex1-dependent functions in neutrophils Molecular Pharmacology 2020 ISSN 1521-0111 Tsitsilashvili, Elene et al. Myelin basic protein charge isomers change macrophage polarization Journal of inflammation research 2019 ISSN 1178--7031 Wu, Xuping et al. Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells Cancer Management and Research 2019 ISSN 1179-1322 Hu, Shuling et al. Mesenchymal Stem Cell Microvesicles Restore Protein Permeability Across Primary Cultures of Injured Human Lung Microvascular Endothelial Cells Stem cells translational medicine 2018 ISSN 2157--6564 Roman-Garcia, Sara et al. Distinct roles for Bruton's Tyrosine Kinase in B cell immune synapse formation Frontiers in Immunology 2018 ISSN 1664-3224 Chen, Lihua et al. Smooth muscle-alpha actin inhibits vascular smooth muscle cell proliferation and migration by inhibiting rac1 activity PLoS ONE 2016 ISSN 1932-6203 Güntert, Tanja et al. Temporal Rac1 - HIF-1 crosstalk modulates hypoxic survival of aged neurons Brain Research 2016 ISSN 1872-6240 Herrera-Martínez, Mayra et al. Antiamoebic activity of Adenophyllum aurantium (L.) strother and its effect on the actin cytoskeleton of Entamoeba histolytica Frontiers in Pharmacology 2016 ISSN 1663-9812 Ahn, Bum Ju et al. Ninjurin1 enhances the basal motility and transendothelial migration of immune cells by inducing protrusive membrane dynamics Journal of Biological Chemistry 2014 ISSN 1083-351X Kalia, Manjula et al. Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism Journal of Virology 2013 ISSN 0022--538X Teplova, Irina et al. ATG proteins mediate efferocytosis and suppress inflammation in mammary involution Autophagy 2013 ISSN 1554-8635 Barrio, Laura et al. TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPases The Journal of Immunology 2013 ISSN 0022--1767 Dhaliwal, Anandika et al. Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases 2012 PMID 22509380 Ninkovi, Jana et al. Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms American Journal of Pathology 2012 ISSN 0002-9440 Ridgway, Lon D. et al. Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells Molecular Cancer Research 2012 ISSN 1541-7786 Vives, Virginie et al. The Rac1 exchange factor Dock5 is essential for bone resorption by osteoclasts 2011 ISSN 1523--4681 Halpert, Michal et al. Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanica Protoplasma 2011 ISSN 0033-183X May, James M. The SLC23 family of ascorbate transporters: ensuring that you get and keep your daily dose of vitamin C British Journal of Pharmacology 2011 ISSN 0007-1188 Lichtenstein, Mathieu P. et al. Secretase-independent and RhoGTPase/PAK/ERK-dependent regulation of cytoskeleton dynamics in astrocytes by NSAIDs and derivatives Journal of Alzheimer's disease : JAD 2010 ISSN 1875--8908 Johanna, Gutiérrez Vargas et al. Rac1 activity changes are associated with neuronal pathology and spatial memory long-term recovery after global cerebral ischemia Neurochemistry international 2010 ISSN 1872--9754 Ridgway, Lon D. et al. Modulation of GEF-H1 Induced Signaling by Heparanase in Brain Metastatic Melanoma Cells Journal of cellular biochemistry 2010 ISSN 0730-2312 Romero, Ana M. et al. Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary culture Toxicological Sciences 2010 ISSN 1096-6080 Fang, Xiaohui et al. Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1 Journal of Biological Chemistry 2010 ISSN 0021-9258 Zhu, Jianxin et al. P21-Activated Kinases Regulate Actin Remodeling in Glomerular Podocytes American Journal of Physiology - Renal Physiology 2010 ISSN 0363-6127 Baumer, Y. et al. Role of Rac 1 and cAMP in endothelial barrier stabilization and thrombin-induced barrier breakdown Journal of Cellular Physiology 2009 ISSN 1097--4652 Chastre, Eric et al. TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness The FASEB Journal 2009 ISSN 1530--6860 Mercer, Jason et al. Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells Science (New York, N.Y.) 2008 ISSN 1095--9203 Pontow, Suzanne et al. Antiviral Activity of a Rac GEF inhibitor Characterized with a Sensitive HIV/SIV Fusion Assay Virology 2007 ISSN 0042-6822
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