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人中性粒细胞胞外捕获(NETs)ELISA试剂盒具有以下优势和特点：
- 即用型方案，带分隔孔，使用方便
- 标准化和高重现性
- 批次间一致性
- 准确性和精确性
通过经典的 ELISA 七点验证人中性粒细胞胞外捕获(NETs)ELISA试剂盒用于评估人血清、血浆、生物液体和细胞培养上清液等样品分析物中的特定生物标记物。该试剂盒采用双抗体间接夹心法--捕获和检测，以确保在估计中性粒细胞胞外捕获物（NETS）时具有高度的灵敏度和特异性。
Pack Type 1 x 96 wells, break-apart wells Species Reactivity Human Sample Type Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only. Assay Range 0.156 – 10 ng/ml Sensitivity 0.055 ng/ml Interference Preparations of the known factors were assayed for interference. No significant interference was observed. Specificity This assay recognises both the recombinant and natural protein for Neutrophil Extracellular Traps (NETS) Principle Of Assay This ELISA is a sandwich immunoassay . Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples. Detection Method Colorimetric, Absorbance measured at 450nm Conjugate-Enzyme Reaction Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development. Quality Certification Validated as per KRISHGEN's Quality Program. GOLD PLUS (GOLD+) validation done. The kit is calibrated against NIBSC calibrator. KRISHGEN Mandate High Performance Assays at Affordable Prices Regulatory Status Research Use Only. Shipping Temperature Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius Storage Temperature The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity. ELISA Type Sandwich ELISA, Double Antibody ELISA Interpretation Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results. Shelf Life Available 8 -10 months at time of shipping Alternate Names / Synonyms Neutrophil Extracellular Traps (NETS); Research Area Immune molecule Disclaimer The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. -
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规格 储存温度
人中性粒细胞胞外捕获(NETs)ELISA试剂盒
KBH4548 96次
-20°C 操作手册
1 1 常温
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保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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