总体RNA甲基化极易定量检测试剂盒（荧光法）

总体RNA甲基化极易定量检测试剂盒（荧光法），非常适合从各种细胞、组织、哺乳动物、真菌、细菌、植物、血浆和血清等样本中提取的总RNA，进行总体水平的RNA甲基化定量分析，整个实验时间仅需2.4小时。主要检测仪器：荧光分光光度计。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

关键词

5-mC

所属分类

RNA修饰检测

目录号

A-P-9009

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产品组分

组分内容		型号：A-P-9009-48(48次)	型号：A-P-9009-96(96次)	储存温度
WB	10X Wash Buffer	14 ml	28 ml	4°C
BS	Binding Solution	5 ml	10 ml	室温
NC	Negative Control,containing 0% 5-mC，50ug/ml *	50 ul	100 ul	-20°C
PC	Positive Control,containing 2% 5-mC，50ug/ml *	10 ul	20 ul	-20°C
mcAb	5-mC Antibody,1000X*	5 ul	10 ul	4°C
SI	Signal Indicator,1000X*	5 ul	10 ul	-20°C
ES	Enhancer Solution,1000X*	5 ul	10 ul	-20°C
FD	Fluoro Developer	12 ul	24 ul	-20°C
FE	Fluoro Enhancer	12 ul	24 ul	-20°C
DB	Dilution Buffer	5 ml	10 ml	室温
8-联管	8-Well Assay Strips(With Frame)	6	12	4°C
操作手册		1	1	室温

*注意：在使用各溶液之前，将溶液离心至管底。注：NC(阴性对照)是未甲基化的RNA，包含0%的5-甲基胞嘧啶(5-mC)。PC(阳性对照)是甲基化的RNA和含有2%的5-甲基胞嘧啶(5-mC)。

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保存建议	推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。
警告	本品仅供科研使用，请勿用于临床与诊断。

FAQ
Fan W et. al. (October 2024). Tet2 modulates M2 macrophage polarization via mRNA 5-methylcytosine in allergic rhinitis. Int Immunopharmacol. 143(Pt 3):113495.

Zhang RK et. al. (October 2024). RNA methyltransferase NSUN2-mediated m5C methylation promotes Cr(VI)-induced malignant transformation and lung cancer by accelerating metabolism reprogramming. Environ Int. 192:109055.

Liu L et. al. (April 2024). YBX1 Promotes Esophageal Squamous Cell Carcinoma Progression via m5C‐Dependent SMOX mRNA Stabilization. Adv Sci (Weinh). :e2302379.

Yu T et. al. (January 2024). Effects of methionine deficiency on B7H3-DAP12-CAR-T cells in the treatment of lung squamous cell carcinoma. Cell Death Dis. 15(1):12.

Huang M et. al. (July 2023). Diagnostic value of aberrant decreased 5-Methylcytosine RNA modification in leukocytes for non-small cell lung cancer. J Cancer. 14(12):2198-2208.

Zhang G et. al. (July 2023). NSUN2 stimulates tumor progression via enhancing TIAM2 mRNA stability in pancreatic cancer. Cell Death Discov. 9(1):219.

Wang Y et. al. (May 2023). Aberrant m5C hypermethylation mediates intrinsic resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant non-small-cell lung cancer. Mol Cancer. 22(1):81.

Betlej G et. al. (October 2022). RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol. 36:100448.

Bataglia L et. al. (July 2021). Active genic machinery for epigenetic RNA modifications in bees. Insect Mol Biol.

Park MH et. al. (January 2020). Mono-(2-Ethylhexyl)phthalate Regulates Cholesterol Efflux via MicroRNAs Regulated m<sup>6</sup>A RNA Methylation. Chem Res Toxicol.

Lewinska A et. al. (August 2017). Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression. Redox Biol. 14:20-34.

Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.

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总体RNA甲基化极易定量检测试剂盒（荧光法）

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