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用 pCMV6-ENTRY 对照组（左泳道）或 pCMV6-ENTRY KRAS（右泳道）cDNA 转染 HEK293T 细胞 48 小时并裂解。等量细胞裂解液（每道 5 微克）经 SDS-PAGE 分离，并用抗 KRAS 免疫印迹。Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
2C1
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Carrier-free.
Immunogen
Full length human recombinant protein of human KRAS (NP_203524) produced in E. coli.
Specificity
Human KRAS
Applications
- IHC - Paraffin (10 µg/ml)
- Western blot (1:2000)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
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产品组分
内容
型号
规格 储存温度
小鼠抗人KRAS单克隆抗体(克隆号2C1) LS-C175665 100ul -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange. Guillard S, Kolasinska-Zwierz P, Debreczeni J, Breed J, Zhang J, Bery N, Marwood R, Tart J, Overman R, Stocki P, Mistry B, Phillips C, Rabbitts T, Jackson R, Minter R. Nature communications. 2017 July;8:16111. (Human)
Full Text Article PubMed: 28706291 PMC: PMC5519984
Antisense oligonucleotide activity in tumour cells is influenced by intracellular LBPA distribution and extracellular vesicle recycling. Alexander N Kapustin, Paul Davey, David Longmire, Carl Matthews, Emily Linnane, Nitin Rustogi, Maria Stavrou, Paul W A Devine, Nicholas J Bond, Lyndsey Hanson, Silvia Sonzini, Alexey Revenko, A Robert MacLeod, Sarah Ross, Elisabetta Chiarparin, Sanyogitta Puri. Communications biology. 2021 Nov;4:1241.
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