- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Unit Definition: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a Δ260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cyclesShelf Life: 12 months
Form: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 20 mM NaCl, 2 mM MgCl2, 1 mM DTT and 50 % [v/v] glycerol)
Concentration: 250 units/μl
Applications:
Turbo Nuclease is very effective in degrading nucleic acid from protein samples and it is used in a variety of application where complete hydrolysis of DNA/RNA is required:- Reduction of viscosity from cell lysates
- Prevention of cell clumping
- Elimination of unspecific protein-DNA complexes prior 2D- or native gel electrophoresis
- Removal of nucleic acids from large-scale protein preparations
Description:
Turbo Nuclease is a broad-spectrum endonuclease that cleaves both DNA and RNA molecules independently of being single- or double-stranded, circular, linear or supercoiled. The enzyme is highly stable and active in a broad range of pH and temperature, making it ideal for a variety of downstream processes that require the degradation of DNA/RNA in a simple, efficient and specific manner.- Endonuclease from Serratia macescens recombinant expressed and purified from E. coli
- Catalytic activity from pH 6 to 10 (optimal around 8.8) and temperature 0 to 44 °C
- High catalytic efficiency (34-fold greater than DNase I)
- Activity requires the presence of Mg2+ (optimum 2 mM)
Procedure:
- Make a fresh, cold lysis buffer in which the target protein is soluble and is compatible with downstream purification processes, e.g. minimal amount of EDTA or DTT if a Ni-NTA column will be used.
- Resuspend the thawed cell paste in lysis buffer. Use 2-10 ml Lysis Buffer for each gram of cell paste.
- Add Turbo Nuclease to 2.5 units/ml.
- A fluid 'aqueous' solution will result after 15 min.
-
产品组分
内容
型号
规格 储存温度
Turbo核酸酶
EN180-01 10000Units
-20°C
Turbo核酸酶
EN180-02 50000Units
-20°C
操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Mass spectrometry reveals novel features of tubulin polyglutamylation in the flagellum of Trypanosoma brucei
Nisavic Marija, Chaze Thibault, ..., Chamot-Rooke Julia
bioRxiv | 2025 Feb 06
Features of membrane protein sequence direct post-translational insertion.
Ilya A Kalinin, Hadas Peled-Zehavi, ..., Nir Fluman
Nature communications | 2024 Nov 25 | 39587101
The long non-coding RNA FAM30A regulates the Musashi2-RUNX1 axis and is required for LSC function in AML cells
Sánchez Jaime Calvo, Scott Mary T., ..., Köhn Marcel
bioRxiv | 2024 Oct 15
Supplementary Gentle Rhodamines for Live-Cell Fluorescence Microscopy
Tianyan Liu, Julian Kompa, ..., Zhixing Chen
ACS Central Science | 2024 Oct 02
APOBEC3C-mediated NF-κB activation enhances clear cell renal cell carcinoma progression.
Nora Hase, Danny Misiak, ..., Marcel Köhn
Molecular oncology | 2024 Aug 26 | 39183666
Biochemical and structural elucidation of the L-carnitine degradation pathway of the human pathogen Acinetobacter baumannii
Fabian Piskol, Peer Lukat, ..., Jürgen Moser
Frontiers in Microbiology | 2024 Aug 14
Rbfox1 controls alternative splicing of focal adhesion genes in cardiac muscle cells.
Peter Zorn, Jaime Calvo Sánchez, ..., Marcel Köhn
Journal of molecular cell biology | 2024 Jul 01 | 38253401
Membrane protein sequence features direct post-translational insertion
Kalinin Ilya A., Peled-Zehavi Hadas, ..., Fluman Nir
bioRxiv | 2023 Dec 12
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
