- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Unit Definition: One unit is defined as the amount of enzyme that catalyses the release of 10 nmol acid-soluble nucleotides in a total reaction volume of 50 μl within 30 minutes at 37 °C.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cyclesShelf Life: 12 months
Form: liquid
Concentration: 20 units/μl
Applications:
- removal of residual ssDNA, including oligonucleotides, from reaction mixtures
- does not degrade double-stranded DNA or RNA
- requires magnesium and presence of free, accessible 3'-hydroxyl-termini
- active in a wide variety of buffer conditions, allowing for direct addition of enzyme to most reaction mixtures
Description:
Catalyses removal of nucleotides from single-stranded DNA in 3' -> 5' direction.10x Reaction Buffer:
670 mM Glycine-KOH (pH 9.5 at 25 °C), 100 mM 2-mercaptoethanol and 67 mM MgCl2.Assay Conditions:
67 mM Glycin-KOH (pH 9.5 at 25 °C), 10 mM 2-mercaptoethanol, 6.7 mM MgCl2, 0.17 mg/ml single-stranded [3H]-DNA. Incubation is at 37 °C for 10 min in a reaction volume of 50 μl. -
产品组分
内容
型号
规格 储存温度
核酸外切酶I
EN177-01 2000Units
-20°C
核酸外切酶I
EN177-02 10000Units
-20°C
操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Selected References:
Lehman et al. (1964) J. Biol. Cem. 239:2628.
Kushne et al. (1971) Proc. Natl. Acad. Sci. USA 68:824.
Kushner et al. (1972) Proc. Natl. Acad. Sci. USA 69:1366.
Goldmark et al. (1972) J. Biol. Chem. 247:184.
Rosamond et al. (1979) J. Biol. Chem. 254:8646.
Werle et al. (1994) Nuc. Acids Research 22 (20):4354.Leaky pollination barrier in edaphic vicarious alpine plants
Thomas Huber, Roman Fuchs, ..., Hanno Schaefer
Europe PMC | 2024 Feb 20
Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal.
Medhavi Dhakal, Tulsi Ram Gompo, ..., Kishor Pandey
Pathogens (Basel, Switzerland) | 2023 Aug 15 | 37624005
Alterations in the hepatocyte epigenetic landscape in steatosis.
Ranjan Kumar Maji, Beate Czepukojc, ..., Alexandra K Kiemer
Epigenetics & chromatin | 2023 Jul 10 | 37415213
Decoding molecular programs in melanoma brain metastases.
Josefine Radke, Elisa Schumann, ..., Torben Redmer
Nature communications | 2022 Nov 29 | 36435874
First report on the molecular detection of Entamoeba bovis from the endangered wild water buffalo ( Bubalus arnee ) in Nepal
Menuka Aryal, Roshan Babu Adhikari, ..., Kishor Pandey
Veterinary Medicine and Science | 2021 Dec 17 | 34919350
Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology.
Sascha Tierling, Kathrin Kattler, ..., Jörn Walter
Open forum infectious diseases | 2021 Aug 01 | 34409122
Fast and Ample Light Controlled Actuation of Monodisperse All‐DNA Microgels
Rémi Merindol, Nicolas Martin, ..., Serge Ravaine
Advanced Functional Materials | 2021 Feb 18
Low genetic diversity of cultivated spotted hard clam ( Meretrix petechialis ) in Taiwan
Han‐Ting Huang, Chia‐Ning Pao, ..., Li‐Lian Liu
Aquaculture Research | 2020 Apr 24
Patent Isothermal nucleic acid amplification
Mark Jay Hoser
us10472659 | 2019 Nov 12
Morphological and Molecular Characterization of Zanthoxylum zanthoxyloides (Rutaceae) from Burkina Faso
Lassané Ouédraogo, Dominik Fuchs, ..., Martin Kiendrebeogo
Plants | 2019 Sep 19 | 31546868
Repeated domestication of melon (Cucumis melo) in Africa and Asia and a new close relative from India.
Josef Endl, Enoch G Achigan-Dako, ..., Hanno Schaefer
American journal of botany | 2019 Jul 12 | 30299543
An extended sequence specificity for UV-induced DNA damage.
Long H Chung, Vincent Murray
Journal of photochemistry and photobiology… | 2018 Jan 30 | 29149689
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
