- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Unit Definition: One unit of rSAP releases 1 μmol phosphate/min from 4-nitrophenyl phosphate in 0.1 M glycine-NaOH pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 and 6 mM 4-nitrophenyl phosphate.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Form: liquid
Concentration: 1 unit/μl
Activity: Optimum working range is between pH 7-9. rSAP is active in most restriction- and PCR-buffers. Mg2+ (>1 mM) is required for activity.
Description:
Recombinant Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that can be fully inactivated by a short heat treatment. This property simplifies most workflows involving alkaline phosphatase treatment. The recombinant form of SAP replaces the native form that has been established on the market for several years. rSAP has all the properties of the well proven SAP, but with additional benefits. rSAP is far more stable at ambient temperature, it is of high, consistent purity, and is available in large batches at high concentration.- Heat-labile, all-purpose alkaline phosphatase
- Fast and easy dephosphorylation of DNA, RNA and nucleotides
- Active in most restriction enzyme buffers, no need for extra addition of buffer or ions
- Excellent stability at 4°C and even room temperature
Heat inactivation:
rSAP is completely inactivated by a 5 min incubation at 65 °C.Absence of contaminants:
Tested for the absence of exonucleases and endonucleases. -
产品组分
内容
型号
规格 储存温度
虾碱性磷酸酶(rSAP)
EN174-01 400Units
-20°C
虾碱性磷酸酶(rSAP)
EN174-02 2000Units
-20°C
操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Selected References:
Chakraborty et al. (2011) Active site detection by spatial conformity and electrostatic analysis-unravelling a proteolytic function in shrimp alkaline phosphatase. PLoS One. 6: e28470.
de Bakcer et al. (2002) The 1.9 A crystal structure of heat-labile shrimp alkaline phosphatase. J Mol Biol. 18:1265.
Nilsen et al. (2001) Thermolabile alkaline phosphatase from Northern shrimp (Pandalus borealis): protein and cDNA sequence analyses. Comp Biochem Physiol B Biochem Mol Biol. 129:853.Characterization of the small Arabidopsis thaliana GTPase and ADP-ribosylation factor-like 2 protein TITAN 5
Inga Mohr, Amin Mirzaiebadizi, ..., Petra Bauer
Journal of Cell Science | 2024 Aug 01 | 39056156
Leaky pollination barrier in edaphic vicarious alpine plants
Thomas Huber, Roman Fuchs, ..., Hanno Schaefer
Europe PMC | 2024 Feb 20
The Structural and Biochemical Characterization of UNC119B Cargo Binding and Release Mechanisms.
Tamas Yelland, Esther Garcia, ..., Shehab Ismail
Biochemistry | 2021 Jun 15 | 34130453
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
