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表皮生长因子受体（EGFR；ErbB-1；HER1）是表皮生长因子家族成员的细胞表面受体。EGFR激酶的过表达和/或过度激活与多种人类癌症相关，如肺癌、胶质母细胞瘤以及头颈部上皮肿瘤，这促使了靶向EGFR的抗癌疗法的研发。EGFR激酶检测试剂盒旨在使用Kinase-Glo® MAX作为检测试剂，测量EGFR激酶活性，用于筛选和分析应用。
Assay Kit Format
Luminescent
Species
Human
Supplied As
The EGFR Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubatorTarget(s)
EGFR
UniProt #
P00533
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产品组分
内容 型号
规格 储存温度
表皮生长因子受体(EGFR)激酶检测试剂盒
40321-01 96次
-20°C 表皮生长因子受体(EGFR)激酶检测试剂盒
40321-02 192次
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)
New Design, synthesis, anticancer evaluation, biological screening, and computational study of novel 6,7-dimethoxyquinazoline derivatives as VEGFR-2 inhibitors and apoptotic inducers.
Mostafa M A Aref, Abdallah E Abdallah, ..., Mohamed Ayman El-Zahabi
European journal of medicinal chemistry | 2025 Sep 23 | 41014811
Design, synthesis and molecular modeling of new coumarin–thiazole derivatives as dual EGFR/HDAC1 inhibitors: in vitro and in vivo anticancer assays
Eman Y. Ahmed, Mai M. Elghonemy, ..., Nehad A. Abdel Latif
RSC Advances | 2025 Sep 11
Chiral 1,3,5-Triazines as Potential EGFR Inhibitors: Pharmacophore Modeling, Synthesis, Molecular Docking, Molecular Dynamics Simulation, and Anticancer Evaluation.
Saravanakumar Natarajan, Latha Ganesapandian, ..., Suresh Palaniswamy
Chemistry & biodiversity | 2025 Aug 29 | 40882039
New quinazoline-[1,2,4]triazolo[3,4- b ][1,3,4]thiadiazines as inhibitors of EGFR: synthesis, anti-breast cancer evaluation and in silico studies
Mahadev Dattatray Bandgar, Sampath Peddapelli, ..., Sirassu Narsimha
RSC Medicinal Chemistry | 2025 Jul 18
Purine–Hydrazone Scaffolds as Potential Dual EGFR/HER2 Inhibitors
Fatemah S. Albalawi, Mashooq A. Bhat, ..., Isolda Romero-Canelon
Pharmaceuticals | 2025 Jul 17
Achillea fragrantissima (Forssk.) Sch. Bip. essential oil inhibits the growth of pancreatic cancer cells via induction of necrosis, sub-G1 arrest, modulation of β-catenin/ERK signalling pathways and p38α MAPK, CDK2, EGFR inhibition.
Mohammed Khaled Bin Break, Weiam Hussein, ..., Maali D Alshammari
Journal of ethnopharmacology | 2025 Jun 24 | 40571228
Synthesis, in vitro and in silico studies of a novel chrysin-ferrocene Schiff base with potent anticancer activity via G1 arrest, caspase-dependent apoptosis and inhibition of topoisomerase II
Mohammed Khaled Bin Break, Siddique Akber Ansari, ..., Hamad M. Alkahtani
Journal of Enzyme Inhibition and Medicinal… | 2025 May 21 | 40396612
Discovery and optimization of 2-pyridones as dual h-DHFR/EGFR TK inhibitors with immunomodulatory potential; design, synthesis, anti-proliferative activity, and apoptosis inducer.
Ahmed Ragab, Reham R Raslan, ..., Nirvana A Gohar
European Journal of Medicinal Chemistry | 2025 May 10 | 40378576
CEG-0598, a novel dual inhibitor of EGFR and C5aR demonstrates in vitro anticancer and antimetastatic activity in prostate cancer cells.
Ayed A Dera, Majed Al Fayi
Discover Oncology | 2025 May 09 | 40343625
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