- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dryShelf Life: 12 months after date of delivery
Molecular Formula: C6H9NO2
Molecular Weight: 127.14 g/mol (free acid)
Exact Mass: 127.06 g/mol (free acid)
CAS#: 942518-19-6
Purity: mass identification conforms (ESI-MS)
Form: solid
Color: off-white to grey
Applications:
Proteins synthesis monitoring[1,2,3]Description:
L-Homopropargylglycine (L-HPG) provides a non-radioactive alternative to analyze the global protein synthesis in cell culture. It is cell-permeable and randomly incorporated instead of methionine during translation[1,2,3]. The resulting alkyne-labeled full-length proteins can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
-
产品组分
内容
型号
规格 储存温度
(S)-2-氨基己-5-炔酸盐酸盐
CLK1067-01 25mg
-20 °C
(S)-2-氨基己-5-炔酸盐酸盐
CLK1067-02 100mg
-20 °C (S)-2-氨基己-5-炔酸盐酸盐
CLK1067-03 1g
-20 °C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Selected References:
[1] Dieck et al. (2012) Metabolic Labeling with Noncanonical Amino Acids and Visualisation by Chemoselective Fluorescent Tagging. Current Protocols in Cell Biology 7:7.11.1.
[2] Kiick et al. (2002) Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation. Proc. Natl. Acad. Sci. USA 99 (1):19.
[3] Dieterich et al. (2010) In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons. Nature Neuroscience 13 (7): 897.
[4] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[5] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.ISQ201, a neuroprotective agent in ischemic stroke, is a novel activator of protein synthesis via allosteric binding to translation factor eIF4E.
Emma Martínez-Alonso, Alejandro Escobar-Peso, ..., Alberto Alcázar
International journal of biological macrom… | 2025 Sep 09 | 40935044
Ultra-parallel ribosome profiling platform with RNA-dependent RNA amplification
Shichino Yuichi, Mito Mari, ..., Iwasaki Shintaro
bioRxiv | 2025 Aug 07
Mammalian cells evacuate and shelter mitochondrial DNA from destruction following hypoxia response-induced mitophagy
Kamegai Reiko, Uenosono Asato, ..., Yanagitani Kota
bioRxiv | 2025 Aug 02
Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.
Geraldine Rodschinka, Sergio Forcelloni, ..., Danny D Nedialkova
Nature structural & molecular biology | 2025 Jul 11 | 40646310
Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts
Wakigawa Taisei, Mito Mari, ..., Iwasaki Shintaro
bioRxiv | 2025 May 03
Selection of initiator tRNA and start codon by mammalian mitochondrial initiation factor 3 in leaderless mRNA translation.
Muhoon Lee, Taisei Wakigawa, ..., Nono Takeuchi-Tomita
Nucleic Acids Research | 2025 Jan 29 | 39878211
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
