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For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C13H19N6O12P3 (free acid)
Molecular Weight: 544.24 g/mol (free acid)
Exact Mass: 544.03 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solid
Color: white to off-white
Solubility: 10 mM Tris-HCl pH 7.5
Spectroscopic Properties: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
in vitro phosphorylation of recombinant proteins[1]Description:
Lee et al.[1] reported a non-radioactive version of in vitro phosphorylation were γ-[(Propargyl)-imido]-ATP (compound 1[1]) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.The phosphorylated, alkyne-modified protein substrate can subsequently be labeled with azides of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry.
Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Please note: This compound contains a phosphoramide linkage which is hydrolyzed at pH <7.
For preparation of a 10 mM solution use 100 mM buffer (for example: bicarbonate, borate, phosphate and Tris) to prevent degradation at acidic pH. -
产品组分
内容
型号
规格 储存温度
γ-[(炔丙基)-酰亚胺]-ATP
CLKT11-01 1mg
-20 °C
γ-[(炔丙基)-酰亚胺]-ATP
CLKT11-02 5mg
-20 °C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
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