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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H13N3O5
Molecular Weight: 267.24 g/mol
Exact Mass: 267.09 g/mol
CAS#: 65223-78-1
Purity: ≥ 99 % (HPLC)
Form: solid
Color: white to off-white
Solubility: DMSO
Spectroscopic Properties: λmax 294 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
RNA synthesis monitoring[1]Description:
Ethynyl-labeled cytidine (5-EC) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EC is cell permeable and incorporates into nascent RNA instead of its natural analog cytidine.The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].
Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
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产品组分
内容
型号
规格 储存温度
5-乙炔基胞苷(5-EC)
CLK087-01 10mg
-20 °C
5-乙炔基胞苷(5-EC)
CLK087-02 50mg
-20 °C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Qu et al. (2013) 5-Ethynylcytidine as a new agent for detecting RNA synthesis in live cells by click chemistry. Anal. Biochem. 434:128.UCK2-dependent conversion of cytidine to CTP is required for CTP uptake by Chlamydia trachomatis
Laure Blanchet, Agathe Subtil
microPublication Biology | 2025 May 09
The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in the sea anemone Nematostella vectensis
Kjosavik Malin A., Garschall Kathrin, Steinmetz Patrick R. H.
bioRxiv | 2024 Oct 22
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